Th Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:286599 Song NN, et al., Rnf220/Zc4h2-mediated monoubiquitylation of Phox2 is required for noradrenergic neuron development. Development. 2020 Mar 30;147(6):dev185199
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:7608333
Gene symbol:	Th
Gene name:	tyrosine hydroxylase

Antibody:	anti-TH (T1299)
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to Alexa Fluor 488

Results

1C Th/Phox2a ©

Specimen 1C Th/Phox2a: embryonic day 16.5 (more

)
Note: Double labeled: green - Th; red - Phox2a (assay MGI:7608328).

Structure	Cell Type	Level	Pattern	Image	Note
TS24: locus coeruleus	noradrenergic neuron	Present		1C Th/Phox2a

Specimen 1C Th/Phox2b: embryonic day 16.5 (more

)
Note: Double labeled: green - Th; red - Phox2b (assay MGI:7608330).

Structure	Cell Type	Level	Pattern	Image	Note
TS24: locus coeruleus	noradrenergic neuron	Present		1C Th/Phox2b`

1C Th/Zc4h2 ©

Specimen 1C Th/Zc4h2: embryonic day 16.5 (more

)
Note: Double labeled: green - Th; red - Zc4h2 (assay MGI:7608332).

Structure	Cell Type	Level	Pattern	Image	Note
TS24: locus coeruleus	noradrenergic neuron	Present		1C Th/Zc4h2

Specimen 4C Control (left): embryonic day 16.5 (more

)
Note: Double labeled: green - Th; red - Prph (assay MGI:7608334).

Structure	Cell Type	Level	Pattern	Image	Note
TS24: locus coeruleus	noradrenergic neuron	Present		4C Control (left)

4C Zc4h2 KO ©

Specimen 4C Zc4h2 KO: embryonic day 16.5; Zc4h2^em1.1Yqd/Zc4h2^em1.1Yqd (more

)
Note: Double labeled: green - Th; red - Prph (assay MGI:7608334).

Structure	Level	Pattern	Image	Note
TS24: locus coeruleus	Absent		4C Zc4h2 KO	Expression completely lost.

Specimen 4C Control (right): embryonic day 16.5 (more

)
Note: Double labeled: green - Th; red - Prph (assay MGI:7608334).

Structure	Cell Type	Level	Pattern	Image	Note
TS24: locus coeruleus	noradrenergic neuron	Present		4C Control (right)

4C Rnf220 KO ©

Specimen 4C Rnf220 KO: embryonic day 16.5; Rnf220^em1.1Kust/Rnf220^em1.1Kust (more

)
Note: Double labeled: green - Th; red - Prph (assay MGI:7608334).

Structure	Level	Pattern	Image	Note
TS24: locus coeruleus	Absent		4C Rnf220 KO	Expression completely lost.

S3B Control ©

Specimen S3B Control: embryonic day 12.5 (more

)
Note: Double labeled: green - Th.

Structure	Cell Type	Level	Pattern	Image	Note
TS20: sympathetic ganglion	noradrenergic neuron	Present		S3B Control

Specimen S3B Rnf220 KO: embryonic day 12.5; Rnf220^em1.1Kust/Rnf220^em1.1Kust (more

)
Note: Double labeled: green - Th.

Structure	Cell Type	Level	Pattern	Image	Note
TS20: sympathetic ganglion	noradrenergic neuron	Present		S3B Rnf220 KO	Expression in peripheral noradrenergic neurons not affected compared to control.

Specimen Zc4h2 KO not shown: embryonic day 12.5; Zc4h2^em1.1Yqd/Zc4h2^em1.1Yqd (more

)
Note: Double labeled: green - Th.

Structure	Cell Type	Level	Pattern	Image	Note
TS20: sympathetic ganglion	noradrenergic neuron	Present			Expression in peripheral noradrenergic neurons not affected compared to control.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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