Gene Expression Data
Assay Details
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Assay
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Reference:
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J:286599
Song NN, et al., Rnf220/Zc4h2-mediated monoubiquitylation of Phox2 is required for noradrenergic neuron development. Development. 2020 Mar 30;147(6):dev185199
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7608332
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Gene symbol:
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Zc4h2
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Gene name:
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zinc finger, C4H2 domain containing
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Antibody:
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anti-Zc4h2
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin coupled to Cy3
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Results
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Specimen
1C Th/Zc4h2:
embryonic day 16.5
(more  )
Note: Double labeled: red - Zc4h2; green - Th (assay MGI:7608333).
Specimen
1C Th/Zc4h2:
(close  )
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Genetic Background:
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involves: C57BL/6
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Zc4h2; green - Th (assay MGI:7608333).
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Specimen
2A Control:
embryonic day 12.0
(more )
Specimen
2A Control:
(close )
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Genetic Background:
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involves: C57BL/6
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Age:
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embryonic day 12.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
2A Zc4h2 KO:
(close )
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Genetic Background:
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involves: C57BL/6
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Mutant Allele(s):
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Zc4h2em1.1Yqd/Zc4h2em1.1Yqd
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Age:
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embryonic day 12.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
3C Zc4h2:
embryonic day 16.5
(more )
Specimen
3C Zc4h2:
(close )
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Genetic Background:
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involves: C57BL/6
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: locus coeruleus |
Strong |
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3C Zc4h2
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Intense expression in locus coeruleus.
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| TS24: pons |
Strong |
Regionally restricted |
3C Zc4h2
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Intense expression in Barrington's nucleus (ventromedial to locus coeruleus).
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Specimen
S1B Zc4h2:
embryonic day 11.5
(more )
Specimen
S1B Zc4h2:
(close )
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Genetic Background:
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involves: C57BL/6
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
S1C Zc4h2/BrdU:
embryonic day 11.5
(more )
Note: Double labeled: red - Zc4h2; green - BrdU.
Specimen
S1C Zc4h2/BrdU:
(close )
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Genetic Background:
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involves: C57BL/6
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Zc4h2; green - BrdU.
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Specimen
S2A Control:
embryonic day 12.5
(more )
Specimen
S2A Control:
(close )
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Genetic Background:
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involves: C57BL/6
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Age:
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embryonic day 12.5
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Sex:
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Female
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: pons |
Present |
Regionally restricted |
S2A Control
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Expression in rostral pons.
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Specimen
S2A Zc4h2+/-:
embryonic day 12.5; Zc4h2em1.1Yqd/Zc4h2 +
(more )
Specimen
S2A Zc4h2+/-:
(close )
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Genetic Background:
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involves: C57BL/6
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Mutant Allele(s):
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Zc4h2em1.1Yqd/Zc4h2+
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Age:
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embryonic day 12.5
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Sex:
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Female
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: pons |
Weak |
Regionally restricted |
S2A Zc2h2+/-
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Reduced number of expressing cells in rostral pons compared to control.
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Specimen
S2B Control:
embryonic day 12.5
(more )
Specimen
S2B Control:
(close )
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Genetic Background:
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involves: C57BL/6
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Age:
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embryonic day 12.5
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Sex:
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Female
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: pons |
Present |
Regionally restricted |
S2B Control
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Expression in rostral pons.
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Specimen
S2B Zc4h2+/+:
embryonic day 12.5; Zc4h2em1.1Yqd/Zc4h2 +
(more )
Specimen
S2B Zc4h2+/+:
(close )
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Genetic Background:
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involves: C57BL/6
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Mutant Allele(s):
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Zc4h2em1.1Yqd/Zc4h2+
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Age:
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embryonic day 12.5
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Sex:
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Female
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: pons |
Weak |
Regionally restricted |
S2B Zc2h2+/-
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Reduced number of expressing cells in rostral pons compared to control.
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Specimen
S2C Control:
embryonic day 12.5
(more )
Specimen
S2C Control:
(close )
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Genetic Background:
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involves: C57BL/6
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Age:
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embryonic day 12.5
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Sex:
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Female
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: pons |
Present |
Regionally restricted |
S2C Control
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Expression in rostral pons.
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Specimen
S2C Zc4h2+/-:
embryonic day 12.5; Zc4h2em1.1Yqd/Zc4h2 +
(more )
Specimen
S2C Zc4h2+/-:
(close )
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Genetic Background:
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involves: C57BL/6
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Mutant Allele(s):
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Zc4h2em1.1Yqd/Zc4h2+
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Age:
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embryonic day 12.5
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Sex:
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Female
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: pons |
Weak |
Regionally restricted |
S2C Zc2h2+/-
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Reduced number of expressing cells in rostral pons compared to control.
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Analysis Tools