Fezf1 RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:308818 Moreau MX, et al., Single-cell transcriptomics of the early developing mouse cerebral cortex disentangle the spatial and temporal components of neuronal fate acquisition. Development. 2021 Jul 15;148(14):dev197962
Assay type:	RNA in situ
MGI Accession ID:	MGI:7606754
Gene symbol:	Fezf1
Gene name:	Fez family zinc finger 1

Probe:	Fezf1 cDNA
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Results

3B Fezf1 ©

Specimen 3B: embryonic day 12.5 (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS20: telencephalon	glutamatergic neuron	Present		3B Fezf1
TS20: neocortex		Present	Regionally restricted	3B Fezf1	Expression was detected in a sharp domain in the ventral aspect of the prospective insular cortex.

4E left ©

4E right ©

Specimen 4E: embryonic day 12.5; Emx1^tm1(cre)Krj/Emx1⁺, Gt(ROSA)26Sor^tm1(EYFP)Cos/Gt(ROSA)26Sor⁺ (more

)
Note: Conditional mutant. Double labeled: magenta - Fezf1; green - Rosa26^YFP.

Structure	Level	Pattern	Image	Note
TS20: pallium	Present	Regionally restricted	4E left , 4E right	Emx1Cre recombines mostly in lateral and dorsal pallium progenitors; Fezf1 signal overlaps with the ventral-most GFP+ area.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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