Probe:	Prmt8 cDNA
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Cell Type	Level	Pattern	Image	Note
TS24: brain	neuron	Present	Non-Uniform	1A	The distribution of expressing neurons was uneven throughout the brain.
TS24: spinal cord	neuron	Present		1A
TS24: brain	glial cell	Absent		1A
TS24: spinal cord	glial cell	Absent		1A

Structure	Cell Type	Level	Pattern	Image	Note
TS28: main olfactory bulb granule cell layer	neuron	Present		2A	Expression is in most neurons.
TS28: main olfactory bulb mitral cell layer	neuron	Present		2A	Expression is in most neurons.
TS28: main olfactory bulb glomerular layer	periglomerular cell	Present		2A	Expression is in periglomerular cells.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: anterior olfactory nucleus	neuron	Present		2B
TS28: neocortex layer	neuron	Present	Regionally restricted	2B	Expression was in neurons distributed evenly throughout but was absent in the molecular layer.
TS28: accessory olfactory bulb	neuron	Absent		2B

Structure	Cell Type	Level	Pattern	Image	Note
TS28: entorhinal cortex	neuron	Strong	Regionally restricted	2C	Expression is in neurons in layer II. Expression is in scattered neurons in layers I and III.
TS28: olfactory tubercle	neuron	Strong	Regionally restricted	2C	Expression is in neurons in layer II. Expression is in scattered neurons in layers I and III.
TS28: piriform cortex	neuron	Strong	Regionally restricted	2C	Expression is in neurons in layer II. Expression is in scattered neurons in layers I and III.
TS28: taenia tecta	neuron	Present		2C	Expression is in all neurons.
TS28: neocortex layer	neuron	Present	Regionally restricted	2C	Expression was in neurons distributed evenly throughout but was absent in the molecular layer.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: entorhinal cortex	neuron	Strong	Regionally restricted	2D	Expression is in neurons in layer II. Expression is in scattered neurons in layers I and III.
TS28: olfactory tubercle	neuron	Strong	Regionally restricted	2D	Expression is in neurons in layer II. Expression is in scattered neurons in layers I and III.
TS28: piriform cortex	neuron	Strong	Regionally restricted	2D	Expression is in neurons in layer II. Expression is in scattered neurons in layers I and III.
TS28: lateral septal nucleus	neuron	Strong	Single cells	2D
TS28: nucleus of diagonal band	neuron	Strong	Single cells	2D
TS28: taenia tecta	neuron	Present		2D	Expression is in all neurons.
TS28: neocortex layer	neuron	Present	Regionally restricted	2D	Expression was in neurons distributed evenly throughout but was absent in the molecular layer.
TS28: medial septal nucleus	neuron	Absent		2D

Structure	Cell Type	Level	Pattern	Image	Note
TS28: bed nucleus of stria terminalis	neuron	Strong		2E
TS28: neocortex layer	neuron	Present	Regionally restricted	2E	Expression was in neurons distributed evenly throughout but was absent in the molecular layer.
TS28: anterior thalamic group		Weak		2E
TS28: globus pallidus		Absent		2E

Structure	Cell Type	Level	Pattern	Image	Note
TS28: paraventricular thalamic nucleus	neuron	Very strong		2F
TS28: lateral geniculate nucleus	neuron	Strong		2F
TS28: ventral posterior lateral thalamic nucleus	neuron	Strong		2F
TS28: ventral posterior medial thalamic nucleus	neuron	Strong		2F
TS28: neocortex layer	neuron	Present	Regionally restricted	2F	Expression was in neurons distributed evenly throughout but was absent in the molecular layer.
TS28: lateral dorsal thalamic nucleus	neuron	Present		2F
TS28: ventromedial hypothalamic nucleus	neuron	Present		2F
TS28: medial dorsal thalamic nucleus	neuron	Moderate		2F
TS28: posterior thalamic group	neuron	Moderate		2F
TS28: reticular thalamic nucleus	neuron	Moderate		2F
TS28: ventral posterior thalamic group	neuron	Moderate		2F
TS28: preoptic area		Absent		2F

Structure	Cell Type	Level	Pattern	Image	Note
TS28: parafascicular nucleus	neuron	Very strong		2G
TS28: CA1 pyramidal cell layer	neuron	Strong		2G
TS28: CA2 pyramidal cell layer	neuron	Strong		2G
TS28: CA3 pyramidal cell layer	neuron	Strong		2G
TS28: amygdala	neuron	Strong		2G
TS28: dentate gyrus granule cell layer	neuron	Strong		2G
TS28: lateral geniculate nucleus	neuron	Strong		2G
TS28: ventral posterior lateral thalamic nucleus	neuron	Strong		2G
TS28: ventral posterior medial thalamic nucleus	neuron	Strong		2G
TS28: retrosplenial cortex	neuron	Strong		2G	Expression is in a large number of neurons.
TS28: subiculum	neuron	Strong		2G	Expression is in a large number of neurons.
TS28: neocortex layer	neuron	Present	Regionally restricted	2G	Expression was in neurons distributed evenly throughout but was absent in the molecular layer.
TS28: posterior thalamic group		Moderate		2G
TS28: reticular thalamic nucleus		Moderate		2G
TS28: ventral posterior thalamic group		Moderate		2G

Structure	Cell Type	Level	Pattern	Image	Note
TS28: lateral geniculate nucleus	neuron	Strong		2H
TS28: medial geniculate nucleus	neuron	Strong		2H
TS28: ventral posterior lateral thalamic nucleus	neuron	Strong		2H
TS28: ventral posterior medial thalamic nucleus	neuron	Strong		2H
TS28: neocortex layer	neuron	Present	Regionally restricted	2H	Expression was in neurons distributed evenly throughout but was absent in the molecular layer.
TS28: superior colliculus	neuron	Present	Regionally restricted	2H	Expression is evenly through the superficial part and is absent in the deep layers.
TS28: nucleus of lateral lemniscus	neuron	Present		2H
TS28: lateral posterior thalamic nucleus		Absent		2H
TS28: locus coeruleus		Absent		2H
TS28: oculomotor nucleus		Absent		2H
TS28: raphe nucleus		Absent		2H
TS28: red nucleus		Absent		2H
TS28: substantia nigra		Absent		2H
TS28: trochlear nucleus		Absent		2H
TS28: ventral tegmental area		Absent		2H

Structure	Cell Type	Level	Pattern	Image	Note
TS28: entorhinal cortex	neuron	Strong	Regionally restricted	2I	Expression is in neurons in layer II. Expression is in scattered neurons in layers I and III.
TS28: olfactory tubercle	neuron	Strong	Regionally restricted	2I	Expression is in neurons in layer II. Expression is in scattered neurons in layers I and III.
TS28: piriform cortex	neuron	Strong	Regionally restricted	2I	Expression is in neurons in layer II. Expression is in scattered neurons in layers I and III.
TS28: taenia tecta	neuron	Present		2I	Expression is in all neurons.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: deep cerebellar nucleus		Strong	Regionally restricted	2J	Expression is in three cerebellar nuclei.
TS28: cerebellum Purkinje cell layer	neuron	Strong		2J
TS28: trigeminal V mesencephalic nucleus	neuron	Strong		2J
TS28: trigeminal V principal sensory nucleus	neuron	Strong		2J
TS28: trigeminal V motor nucleus		Present		2J
TS28: cerebellum granule cell layer		Absent		2J
TS28: facial nucleus		Absent		2J
TS28: nucleus ambiguus		Absent		2J
TS28: nucleus of trapezoid body		Absent		2J
TS28: superior olivary nucleus		Absent		2J
TS28: suprachiasmatic nucleus		Absent		2J

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer		Strong		2K
TS28: dorsal cochlear nucleus		Strong		2K
TS28: ventral cochlear nucleus		Strong		2K
TS28: deep cerebellar nucleus		Strong	Regionally restricted	2K	Expression is in three cerebellar nuclei.
TS28: vestibular nucleus	neuron	Present		2K
TS28: medial vestibular nucleus	neuron	Present	Regionally restricted	2K	Expression is concentrated in the rostral two-third of the nucleus.
TS28: cerebellum granule cell layer		Absent		2K

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	neuron	Strong		2L
TS28: inferior olivary nucleus	neuron	Strong		2L
TS28: deep cerebellar nucleus		Strong	Regionally restricted	2L	Expression is in three cerebellar nuclei.
TS28: trigeminal V spinal sensory nucleus	neuron	Strong	Regionally restricted	2L	Expression is throughout the nucleus from the superficial portion to the medial border of the magnocellular portion.
TS28: inferior colliculus	neuron	Present	Uniform	2L
TS28: cerebellum granule cell layer		Absent		2L

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer		Strong		2M
TS28: deep cerebellar nucleus		Strong	Regionally restricted	2M	Expression is in three cerebellar nuclei.
TS28: trigeminal V spinal sensory nucleus	neuron	Strong	Regionally restricted	2M	Expression is throughout the nucleus from the superficial portion to the medial border of the magnocellular portion.
TS28: cuneate nucleus	neuron	Present	Regionally restricted	2M	Expression is in a group.
TS28: gracile nucleus	neuron	Present	Regionally restricted	2M	Expression is in a group.
TS28: lateral reticular nucleus		Present		2M
TS28: abducens nucleus		Absent		2M
TS28: cerebellum granule cell layer		Absent		2M
TS28: hypoglossal nucleus		Absent		2M
TS28: pontine nucleus		Absent		2M
TS28: reticular tegmental nucleus		Absent		2M
TS28: solitary tract nucleus		Absent		2M

Structure	Cell Type	Level	Pattern	Image	Note
TS28: retrosplenial cortex	neuron	Strong		3A	Expression is in a large number of neurons.
TS28: subiculum	neuron	Strong		3A	Expression is in a large number of neurons.
TS28: CA1 pyramidal cell layer		Strong		3A
TS28: CA2 pyramidal cell layer		Strong		3A
TS28: CA3 pyramidal cell layer		Strong		3A
TS28: dentate gyrus granule cell layer		Strong		3A

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Strong		3B
TS28: deep cerebellar nucleus	Strong	Regionally restricted	3B	Expression is in three cerebellar nuclei.
TS28: cerebellum granule cell layer	Absent		3B

Structure	Cell Type	Level	Pattern	Image	Note
TS28: paraventricular thalamic nucleus	neuron	Very strong		4
TS28: accumbens nucleus	neuron	Strong		4
TS28: caudate-putamen	neuron	Strong		4
TS28: ventral posterior lateral thalamic nucleus	neuron	Strong		4
TS28: ventral posterior medial thalamic nucleus	neuron	Strong		4
TS28: lateral dorsal thalamic nucleus	neuron	Present		4
TS28: subthalamic nucleus	neuron	Present	Single cells	4
TS28: medial dorsal thalamic nucleus		Moderate		4
TS28: posterior thalamic group		Moderate		4
TS28: reticular thalamic nucleus		Moderate		4
TS28: ventral posterior thalamic group		Moderate		4
TS28: habenula		Absent		4