About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:276571 Rajderkar S, et al., Trim33 is required for appropriate development of pre-cardiogenic mesoderm. Dev Biol. 2019 Jun 15;450(2):101-114
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7595622
Gene symbol: Trim33
Gene name: tripartite motif-containing 33
Antibody: anti-Trim33 (HPA004345)
Detection system: Secondary antibody coupled to Alexa Fluor 594
Results
Specimen 1A: embryonic day 6.5; Trim33tm1Vk/Trim33+ (more )
Structure Level Pattern Image Note
TS9: embryo ectoderm Strong Regionally restricted 1A Strong nuclear expression in all epiblast cells.
TS9: primitive endoderm Weak Scattered 1A Scattered weak expression in visceral endoderm.
Specimen 1B: embryonic day 7.0; Trim33tm1Vk/Trim33+ (more )
Note: Double labeled: red - Trim33; green - T (assay MGI:7595623).
Structure Level Pattern Image Note
TS10: embryo Present Regionally restricted 1B (middle) , 1B (left) Nuclear expression both in T-expressing cells undergoing induction and in T-negative cells.
Specimen 1C: embryonic day 7.5; Trim33tm1Vk/Trim33+ (more )
Note: Double labeled: red - Trim33; green - T (assay MGI:7595623).
Structure Level Pattern Image Note
TS11: embryo ectoderm Strong Regionally restricted 1C (left) , 1C (middle) Strong nuclear expression in all ectoderm and mesoderm cells.
TS11: embryo mesoderm Strong Regionally restricted 1C (left) , 1C (middle) Strong nuclear expression in all ectoderm and mesoderm cells.
TS11: embryo endoderm Absent 1C (left) , 1C (middle) Endoderm is mostly negative for expression.
Specimen 1C (far right): embryonic day 7.5; Trim33tm1Vk/Trim33+ (more )
Structure Level Pattern Image Note
TS11: allantois Present Regionally restricted 1C (far right) Nuclear expression in many extraembryonic tissues
TS11: amnion ectoderm Present Regionally restricted 1C (far right) Nuclear expression in many extraembryonic tissues
TS11: chorion Present Regionally restricted 1C (far right) Nuclear expression in many extraembryonic tissues
Specimen 3C: embryonic day 6.5; Trim33tm1Vk/Trim33+ (more )
Note: Double labeled: red - Trim33; green - T (assay MGI:7595623).
Structure Level Pattern Image Note
TS9: embryo ectoderm Present 3C
Specimen 3D: embryonic day 6.5; Edil3Tg(Sox2-cre)1Amc/Edil3+, Trim33tm1Vk/Trim33tm1Vk (more )
Note: Conditional mutant. Double labeled: red - Trim33; green - T (assay MGI:7595623).
Structure Level Pattern Image Note
TS9: embryo ectoderm Trace 3D Expression already ablated at pre-streak stage before mesendoderm induction.
Specimen 3E: embryonic day 7.0; Trim33tm1Vk/Trim33+ (more )
Note: Double labeled: red - Trim33; green - T (assay MGI:7595623).
Structure Level Pattern Image Note
TS10: embryo ectoderm Present 3E
Specimen 3F: embryonic day 7.0; Edil3Tg(Sox2-cre)1Amc/Edil3+, Trim33tm1Vk/Trim33tm1Vk (more )
Note: Conditional mutant. Double labeled: red - Trim33; green - T (assay MGI:7595623).
Structure Level Pattern Image Note
TS10: embryo ectoderm Absent 3F
Specimen 3G: embryonic day 7.5; Trim33tm1Vk/Trim33+ (more )
Note: Double labeled: red - Trim33; green - T (assay MGI:7595623).
Structure Level Pattern Image Note
TS11: embryo ectoderm Present 3G
Specimen 3H: embryonic day 7.5; Edil3Tg(Sox2-cre)1Amc/Edil3+, Trim33tm1Vk/Trim33tm1Vk (more )
Note: Conditional mutant. Double labeled: red - Trim33; green - T (assay MGI:7595623).
Structure Level Pattern Image Note
TS11: embryo ectoderm Absent 3H
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
04/30/2024
MGI 6.23 		The Jackson Laboratory