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Gene Expression Data
Assay Details
Assay
Reference: J:276571 Rajderkar S, et al., Trim33 is required for appropriate development of pre-cardiogenic mesoderm. Dev Biol. 2019 Jun 15;450(2):101-114
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7595623
Gene symbol: T
Gene name: brachyury, T-box transcription factor T
Antibody: anti-Brachyury (AF2085)
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen 1B: embryonic day 7.0; Trim33tm1Vk/Trim33+ (more )
Note: Double labeled: green - T; red - Trim33 (assay MGI:7595622).
Structure Level Pattern Image Note
TS10: mesendoderm Present 1B (left) , 1B (right) Expression in cells undergoing induction.
TS10: embryo ectoderm Absent 1B (left) , 1B (right) No expression in ectoderm cells.
Specimen 1C: embryonic day 7.5; Trim33tm1Vk/Trim33+ (more )
Note: Double labeled: green - T; red - Trim33 (assay MGI:7595622).
Structure Level Pattern Image Note
TS11: mesendoderm Present 1C (right) , 1C (left)
Specimen 3C: embryonic day 6.5; Trim33tm1Vk/Trim33+ (more )
Note: Double labeled: green - T; red - Trim33 (assay MGI:7595622).
Structure Level Pattern Image Note
TS9: embryo Present Regionally restricted 3C Expression in mesendoderm.
Specimen 3D: embryonic day 6.5; Edil3Tg(Sox2-cre)1Amc/Edil3+, Trim33tm1Vk/Trim33tm1Vk (more )
Note: Conditional mutant. Double labeled: green - T; red - Trim33 (assay MGI:7595622).
Structure Level Pattern Image Note
TS9: embryo Present Regionally restricted 3D Expression in mesendoderm.
Specimen 3E: embryonic day 7.0; Trim33tm1Vk/Trim33+ (more )
Note: Double labeled: green - T; red - Trim33 (assay MGI:7595622).
Structure Level Pattern Image Note
TS10: mesendoderm Present 3E
Specimen 3F: embryonic day 7.0; Edil3Tg(Sox2-cre)1Amc/Edil3+, Trim33tm1Vk/Trim33tm1Vk (more )
Note: Conditional mutant. Double labeled: green - T; red - Trim33 (assay MGI:7595622).
Structure Level Pattern Image Note
TS10: mesendoderm Present 3F
Specimen 3G: embryonic day 7.5; Trim33tm1Vk/Trim33+ (more )
Note: Double labeled: green - T; red - Trim33 (assay MGI:7595622).
Structure Level Pattern Image Note
TS11: mesendoderm Present 3G
Specimen 3H: embryonic day 7.5; Edil3Tg(Sox2-cre)1Amc/Edil3+, Trim33tm1Vk/Trim33tm1Vk (more )
Note: Conditional mutant. Double labeled: green - T; red - Trim33 (assay MGI:7595622).
Structure Level Pattern Image Note
TS11: mesendoderm Present 3H
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/14/2024
MGI 6.23 		The Jackson Laboratory