Gene Expression Data
Assay Details
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Assay
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Reference:
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J:332652
Zhu Q, et al., Decoding anterior-posterior axis emergence among mouse, monkey, and human embryos. Dev Cell. 2023 Jan 9;58(1):63-79.e4
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7581003
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Gene symbol:
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Gpr83
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Gene name:
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G protein-coupled receptor 83
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Antibody:
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anti-GPR83 (extracellular) (AGR-053)
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Detection system:
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Secondary antibody
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Assay notes:
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Two Gpr83 antibodies were described, but the one used in this assay was not detailed. Anti-GPR83 MGI:7580958 may have been used in this assay.
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Results
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Specimen
4L:
embryonic day 5.5
(more  )
Note: Double labeled: green - Gpr83; red - Cer1 (assay MGI:7581000).
Specimen
4L:
(close  )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 5.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: green - Gpr83; red - Cer1 (assay MGI:7581000).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS7: primitive endoderm |
Present |
Regionally restricted |
4L Gpr83
,
4L inset
,
4L Cer1/Gpr83
,
4L merge
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Expression in a sub-population of Cer1-expressiong cells, possibly pre-anterior visceral endoderm (pre-AVE), located on distal side of possible distal visceral endoderm (DVE)
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Specimen
S4A:
embryonic day 5.5
(more )
Note: Double labeled: green - Gpr83; red - Cer1 (assay MGI:7581000).
Specimen
S4A:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 5.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: green - Gpr83; red - Cer1 (assay MGI:7581000).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS7: primitive endoderm |
Present |
Regionally restricted |
S4A Gpr83
,
S4A inset
,
S4A Cer1/Gpr83
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S4A merge
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Expression in a sub-population of Cer1-expressiong cells, possibly pre-anterior visceral endoderm (pre-AVE), located on distal side of possible distal visceral endoderm (DVE)
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Specimen
5I:
embryonic day 4.5
(more )
Note: Double labeled: green - Gpr83; cyan - Gata4 (assay MGI:7581006).
Specimen
5I:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 4.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: green - Gpr83; cyan - Gata4 (assay MGI:7581006).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS6: primitive endoderm |
Present |
Regionally restricted |
5I Gpr83
,
5I Gata4/Gpr83
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Expression in pro-anterior visceral endoderm (pro-AVE), possibly within layer of primitive endoderm (PrE).
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Specimen
S4F:
embryonic day 4.5
(more )
Note: Double labeled: green - Gpr83; cyan - Gata4 (assay MGI:7581006).
Specimen
S4F:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 4.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: green - Gpr83; cyan - Gata4 (assay MGI:7581006).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS6: primitive endoderm |
Present |
Regionally restricted |
S4F Gpr83
,
S4F Gata4/Gpr83
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Expression in pro-anterior visceral endoderm (pro-AVE), possibly within layer of primitive endoderm (PrE).
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Analysis Tools