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Gene Expression Data
Assay Details
Assay
Reference: J:333832 Benavente-Diaz M, et al., Dynamics of myogenic differentiation using a novel Myogenin knock-in reporter mouse. Skelet Muscle. 2021 Feb 18;11(1):5
Assay type: In situ reporter (knock in)
MGI Accession ID: MGI:7580772
Gene symbol: Myog
Gene name: myogenin
Reporter: tdTomato
Antibody: anti-DsRed (632496)
Detection system: Secondary antibody coupled to Alexa Fluor
Results
Specimen 2A MyogtdTom/+: embryonic day 10.5; Myogem1.1Tajb/Myog+ (more )
Note: Double labeled: red - Myog reporter; green - Myog protein (assay MGI:7580771).
Structure Level Pattern Image Note
TS17: somite Present 2A MyogtdTom/+ 1st somites tdTOM , 2A MyogtdTom/+ , 2A MyogtdTom/+ interlimb tdTOM Expression was detected in the epaxial and hypaxial domains of all somites. Expression overlapped with the endogenous immunostaining.
Specimen 2A MyogtdTom/tdTom: embryonic day 10.5; Myogem1.1Tajb/Myogem1.1Tajb (more )
Note: Double labeled: red - Myog reporter; green - Myog protein (assay MGI:7580771).
Structure Level Pattern Image Note
TS17: somite Present 2A MyogtdTom/tdTom , 2A MyogtdTom/tdTom interlimb tdTOM , 2A MyogtdTom/tdTom 1st somites tdTOM Expression was detected in the epaxial and hypaxial domains of all somites. Expression overlapped with the endogenous immunostaining.
Specimen 2B EOM: embryonic day 12.5; Myogem1.1Tajb/Myog+ (more )
Note: Double labeled: red - Myog reporter; green - Myog protein (assay MGI:7580771).
Structure Level Pattern Image Note
TS21: eye skeletal muscle Present Regionally restricted 2B EOM merge , 2B EOM tdTOM Expression was detected in extraocular muscle, where there was a 97% co-localization with the endogenous immunostaining.
Specimen 2B Tongue: embryonic day 12.5; Myogem1.1Tajb/Myog+ (more )
Note: Double labeled: red - Myog reporter; green - Myog protein (assay MGI:7580771).
Structure Level Pattern Image Note
TS20: tongue muscle Present 2B Tongue tdTOM , 2B Tongue merge There was a 97% co-localization with the endogenous immunostaining.
Specimen 2B Limb: embryonic day 12.5; Myogem1.1Tajb/Myog+ (more )
Note: Double labeled: red - Myog reporter; green - Myog protein (assay MGI:7580771).
Structure Level Pattern Image Note
TS21: limb muscle Present 2B Limb tdTOM , 2B Limb merge There was a 97% co-localization with the endogenous immunostaining.
Specimen S2A EOM: embryonic day 14.5; Myogem1.1Tajb/Myog+ (more )
Note: Double labeled: red - Myog reporter; green - Myog protein (assay MGI:7580771).
Structure Level Pattern Image Note
TS22: extrinsic ocular muscle Present S2A EOM tdTOM , S2A EOM merge There was a 95% co-localization with the endogenous immunostaining.
Specimen S2A Tongue: embryonic day 14.5; Myogem1.1Tajb/Myog+ (more )
Note: Double labeled: red - Myog reporter; green - Myog protein (assay MGI:7580771).
Structure Level Pattern Image Note
TS22: tongue muscle Present S2A Tongue tdTOM , S2A Tongue merge There was a 95% co-localization with the endogenous immunostaining.
Specimen S2A Limb: embryonic day 14.5; Myogem1.1Tajb/Myog+ (more )
Note: Double labeled: red - Myog reporter; green - Myog protein (assay MGI:7580771).
Structure Level Pattern Image Note
TS22: limb muscle Present S2A Limb merge , S2A Limb tdTOM There was a 95% co-localization with the endogenous immunostaining.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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