|
Results
|
Specimen
3A E12 WT:
embryonic day 12.5
(more  )
Note: late of the day (16-17)
Specimen
3A E12 WT:
(close  )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 12.5
|
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
|
Sex:
|
Female
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
|
Note:
|
late of the day (16-17)
|
Specimen
3A E12 KO:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd
|
|
Mutant Allele(s):
|
Sema6aGt(KST069)Byg/Sema6aGt(KST069)Byg
|
|
Age:
|
embryonic day 12.5
|
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
|
Sex:
|
Female
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
|
Note:
|
late of the day (16-17)
|
Specimen
3A E13 WT:
embryonic day 13.5
(more )
Note: late of the day (16-17)
Specimen
3A E13 WT:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 13.5
|
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
|
Sex:
|
Female
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
|
Note:
|
late of the day (16-17)
|
Specimen
3A E13 KO:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd
|
|
Mutant Allele(s):
|
Sema6aGt(KST069)Byg/Sema6aGt(KST069)Byg
|
|
Age:
|
embryonic day 13.5
|
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
|
Sex:
|
Female
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
|
Note:
|
late of the day (16-17)
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS21: mammary mesenchyme |
Present |
Regionally restricted |
3A E13 KO
|
Increased sensory neuron innervations, compared with the wild-type female (quantified in Fig 3B).
|
| TS21: spinal nerve |
Present |
Regionally restricted |
3A E13 KO
|
Increased sensory neuron innervations, compared with the wild-type female (quantified in Fig 3B).
|
Specimen
3A E14 WT:
embryonic day 14.5
(more )
Note: late of the day (16-17)
Specimen
3A E14 WT:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 14.5
|
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
|
Sex:
|
Female
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
|
Note:
|
late of the day (16-17)
|
Specimen
3A E14 KO:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd
|
|
Mutant Allele(s):
|
Sema6aGt(KST069)Byg/Sema6aGt(KST069)Byg
|
|
Age:
|
embryonic day 14.5
|
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
|
Sex:
|
Female
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
|
Note:
|
late of the day (16-17)
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: intercostal nerve |
Present |
Regionally restricted |
3A E14 KO
|
Increased sensory neuron innervations, compared with the wild-type female (quantified in Fig 3B).
|
| TS22: mammary mesenchyme |
Present |
Regionally restricted |
3A E14 KO
|
Increased sensory neuron innervations, compared with the wild-type female (quantified in Fig 3B).
|
Specimen
3-S1A E12 WT:
embryonic day 12.5
(more )
Note: late of the day (16-17)
Specimen
3-S1A E12 WT:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 12.5
|
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
|
Sex:
|
Male
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
|
Note:
|
late of the day (16-17)
|
Specimen
3-S1A E12 KO:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd
|
|
Mutant Allele(s):
|
Sema6aGt(KST069)Byg/Sema6aGt(KST069)Byg
|
|
Age:
|
embryonic day 12.5
|
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
|
Sex:
|
Male
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
|
Note:
|
late of the day (16-17)
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS20: mammary gland primordium |
Present |
Regionally restricted |
3-S1A E12 KO
|
Sensory neuron innervations were seen, similar as in the wild-type male (quantified in Fig 3-S1B).
|
| TS20: spinal nerve |
Present |
Regionally restricted |
3-S1A E12 KO
|
Sensory neuron innervations were seen, similar as in the wild-type male (quantified in Fig 3-S1B).
|
Specimen
3-S1A E13 WT:
embryonic day 13.5
(more )
Note: late of the day (16-17)
Specimen
3-S1A E13 WT:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 13.5
|
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
|
Sex:
|
Male
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
|
Note:
|
late of the day (16-17)
|
Specimen
3-S1A E13 KO:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd
|
|
Mutant Allele(s):
|
Sema6aGt(KST069)Byg/Sema6aGt(KST069)Byg
|
|
Age:
|
embryonic day 13.5
|
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
|
Sex:
|
Male
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
|
Note:
|
late of the day (16-17)
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS21: mammary mesenchyme |
Present |
Regionally restricted |
3-S1A E13 KO
|
Sensory neuron innervations were seen, similar as in the wild-type male (quantified in Fig 3-S1B).
|
| TS21: spinal nerve |
Present |
Regionally restricted |
3-S1A E13 KO
|
Sensory neuron innervations were seen, similar as in the wild-type male (quantified in Fig 3-S1B).
|
Specimen
4A E12 WT:
embryonic day 12.5
(more )
Note: late of the day (16-17)
Specimen
4A E12 WT:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 12.5
|
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
|
Sex:
|
Female
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
|
Note:
|
late of the day (16-17)
|
Specimen
4A E12 KO:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd
|
|
Mutant Allele(s):
|
Plxna4tm1Matl/Plxna4tm1Matl
|
|
Age:
|
embryonic day 12.5
|
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
|
Sex:
|
Female
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
|
Note:
|
late of the day (16-17)
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS20: mammary gland primordium |
Present |
Regionally restricted |
4A E12 KO
|
Highly increased sensory neuron innervations, compared with the wild-type female (quantified in Fig 4B).
|
| TS20: spinal nerve |
Present |
Regionally restricted |
4A E12 KO
|
Highly increased sensory neuron innervations, compared with the wild-type female (quantified in Fig 4B).
|
Specimen
4A E12 Het:
embryonic day 12.5; Bdnftm1Jae/Bdnf +
(more )
Note: late of the day (16-17)
Specimen
4A E12 Het:
(close )
|
Genetic Background:
|
involves: 129S4/SvJae
|
|
Mutant Allele(s):
|
Bdnftm1Jae/Bdnf+
|
|
Age:
|
embryonic day 12.5
|
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
|
Sex:
|
Female
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
|
Note:
|
late of the day (16-17)
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS20: mammary gland primordium |
Present |
Regionally restricted |
4A E12 Het
|
Decreased sensory neuron innervations, compared with the wild-type female (quantified in Fig 4C).
|
| TS20: spinal nerve |
Present |
Regionally restricted |
4A E12 Het
|
Decreased sensory neuron innervations, compared with the wild-type female (quantified in Fig 4C).
|
Specimen
4A E12 KO/Het:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd * 129S4/SvJae
|
|
Mutant Allele(s):
|
Bdnftm1Jae/Bdnf+
Plxna4tm1Matl/Plxna4tm1Matl
|
|
Age:
|
embryonic day 12.5
|
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
|
Sex:
|
Female
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
|
Note:
|
late of the day (16-17)
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS20: mammary gland primordium |
Present |
Regionally restricted |
4A E12 KO/Het
|
Increased sensory neuron innervations, compared with the wild-type female (quantified in Fig 4D). There was no effect on the innervation density of the gland compared to PlexinA4 KO.
|
| TS20: spinal nerve |
Present |
Regionally restricted |
4A E12 KO/Het
|
Increased sensory neuron innervations, compared with the wild-type female (quantified in Fig 4D). There was no effect on the innervation density of the gland compared to PlexinA4 KO.
|
Specimen
4A E13 WT:
embryonic day 13.5
(more )
Note: late of the day (16-17)
Specimen
4A E13 WT:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 13.5
|
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
|
Sex:
|
Female
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
|
Note:
|
late of the day (16-17)
|
Specimen
4A E13 KO:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd
|
|
Mutant Allele(s):
|
Plxna4tm1Matl/Plxna4tm1Matl
|
|
Age:
|
embryonic day 13.5
|
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
|
Sex:
|
Female
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
|
Note:
|
late of the day (16-17)
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS21: mammary mesenchyme |
Present |
Regionally restricted |
4A E13 KO
|
Highly increased sensory neuron innervations, compared with the wild-type female (quantified in Fig 4B).
|
| TS21: spinal nerve |
Present |
Regionally restricted |
4A E13 KO
|
Highly increased sensory neuron innervations, compared with the wild-type female (quantified in Fig 4B).
|
Specimen
4A E13 Het:
embryonic day 13.5; Bdnftm1Jae/Bdnf +
(more )
Note: late of the day (16-17)
Specimen
4A E13 Het:
(close )
|
Genetic Background:
|
involves: 129S4/SvJae
|
|
Mutant Allele(s):
|
Bdnftm1Jae/Bdnf+
|
|
Age:
|
embryonic day 13.5
|
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
|
Sex:
|
Female
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
|
Note:
|
late of the day (16-17)
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS21: mammary mesenchyme |
Present |
Regionally restricted |
4A E13 Het
|
Decreased sensory neuron innervations, compared with the wild-type female (quantified in Fig 4C).
|
| TS21: spinal nerve |
Present |
Regionally restricted |
4A E13 Het
|
Decreased sensory neuron innervations, compared with the wild-type female (quantified in Fig 4C).
|
Specimen
4A E13 KO/Het:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd * 129S4/SvJae
|
|
Mutant Allele(s):
|
Bdnftm1Jae/Bdnf+
Plxna4tm1Matl/Plxna4tm1Matl
|
|
Age:
|
embryonic day 13.5
|
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
|
Sex:
|
Female
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
|
Note:
|
late of the day (16-17)
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS21: mammary mesenchyme |
Present |
Regionally restricted |
4A E13 KO/Het
|
Increased sensory neuron innervations, compared with the wild-type female (quantified in Fig 4D). Partially suppressed the hyperinnervation caused by PlexinA4 KO.
|
| TS21: spinal nerve |
Present |
Regionally restricted |
4A E13 KO/Het
|
Increased sensory neuron innervations, compared with the wild-type female (quantified in Fig 4D). Partially suppressed the hyperinnervation caused by PlexinA4 KO.
|
Specimen
4A E14 WT:
embryonic day 14.5
(more )
Note: late of the day (16-17)
Specimen
4A E14 WT:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 14.5
|
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
|
Sex:
|
Female
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
|
Note:
|
late of the day (16-17)
|
Specimen
4A E14 KO:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd
|
|
Mutant Allele(s):
|
Plxna4tm1Matl/Plxna4tm1Matl
|
|
Age:
|
embryonic day 14.5
|
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
|
Sex:
|
Female
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
|
Note:
|
late of the day (16-17)
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: intercostal nerve |
Present |
Regionally restricted |
4A E14 KO
|
Increased sensory neuron innervations, compared with the wild-type female (quantified in Fig 4B).
|
| TS22: mammary mesenchyme |
Present |
Regionally restricted |
4A E14 KO
|
Increased sensory neuron innervations, compared with the wild-type female (quantified in Fig 4B).
|
Specimen
4A E14 Het:
embryonic day 14.5; Bdnftm1Jae/Bdnf +
(more )
Note: late of the day (16-17)
Specimen
4A E14 Het:
(close )
|
Genetic Background:
|
involves: 129S4/SvJae
|
|
Mutant Allele(s):
|
Bdnftm1Jae/Bdnf+
|
|
Age:
|
embryonic day 14.5
|
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
|
Sex:
|
Female
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
|
Note:
|
late of the day (16-17)
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: intercostal nerve |
Present |
Regionally restricted |
4A E14 Het
|
Decreased sensory neuron innervations, compared with the wild-type female (quantified in Fig 4C).
|
| TS22: mammary mesenchyme |
Present |
Regionally restricted |
4A E14 Het
|
Decreased sensory neuron innervations, compared with the wild-type female (quantified in Fig 4C).
|
Specimen
4A E14 KO/Het:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd * 129S4/SvJae
|
|
Mutant Allele(s):
|
Bdnftm1Jae/Bdnf+
Plxna4tm1Matl/Plxna4tm1Matl
|
|
Age:
|
embryonic day 14.5
|
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
|
Sex:
|
Female
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
|
Note:
|
late of the day (16-17)
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: intercostal nerve |
Present |
Regionally restricted |
4A E14 KO/Het
|
Similar sensory neuron innervations, compared with the wild-type female (quantified in Fig 4D). Suppressed the hyperinnervation caused by PlexinA4 KO.
|
| TS22: mammary mesenchyme |
Present |
Regionally restricted |
4A E14 KO/Het
|
Similar sensory neuron innervations, compared with the wild-type female (quantified in Fig 4D). Suppressed the hyperinnervation caused by PlexinA4 KO.
|
Specimen
5A E12 WT:
embryonic day 12.5
(more )
Note: late of the day (16-17)
Specimen
5A E12 WT:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 12.5
|
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
|
Sex:
|
Male
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
|
Note:
|
late of the day (16-17)
|
Specimen
5A E12 KO:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd
|
|
Mutant Allele(s):
|
Plxna4tm1Matl/Plxna4tm1Matl
|
|
Age:
|
embryonic day 12.5
|
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
|
Sex:
|
Male
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
|
Note:
|
late of the day (16-17)
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS20: mammary gland primordium |
Present |
Regionally restricted |
5A E12 KO
|
Highly increased sensory neuron innervations, compared with the wild-type male (quantified in Fig 5B).
|
| TS20: spinal nerve |
Present |
Regionally restricted |
5A E12 KO
|
Highly increased sensory neuron innervations, compared with the wild-type male (quantified in Fig 5B).
|
Specimen
5A E12 Het:
embryonic day 12.5; Bdnftm1Jae/Bdnf +
(more )
Note: late of the day (16-17)
Specimen
5A E12 Het:
(close )
|
Genetic Background:
|
involves: 129S4/SvJae
|
|
Mutant Allele(s):
|
Bdnftm1Jae/Bdnf+
|
|
Age:
|
embryonic day 12.5
|
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
|
Sex:
|
Male
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
|
Note:
|
late of the day (16-17)
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS20: mammary gland primordium |
Present |
Regionally restricted |
5A E12 Het
|
Decreased sensory neuron innervations, compared with the wild-type male (quantified in Fig 5C).
|
| TS20: spinal nerve |
Present |
Regionally restricted |
5A E12 Het
|
Decreased sensory neuron innervations, compared with the wild-type male (quantified in Fig 5C).
|
Specimen
5A E12 KO/Het:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd * 129S4/SvJae
|
|
Mutant Allele(s):
|
Bdnftm1Jae/Bdnf+
Plxna4tm1Matl/Plxna4tm1Matl
|
|
Age:
|
embryonic day 12.5
|
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
|
Sex:
|
Male
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
|
Note:
|
late of the day (16-17)
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS20: mammary gland primordium |
Present |
Regionally restricted |
5A E12 KO/Het
|
Increased sensory neuron innervations, compared with the wild-type female (quantified in Fig 5D). There was also a decrease (about 50%) on the innervation density of the gland compared to PlexinA4 KO.
|
| TS20: spinal nerve |
Present |
Regionally restricted |
5A E12 KO/Het
|
Increased sensory neuron innervations, compared with the wild-type female (quantified in Fig 5D). There was also a decrease (about 50%) on the innervation density of the gland compared to PlexinA4 KO.
|
Specimen
5A E13 WT:
embryonic day 13.5
(more )
Note: late of the day (16-17)
Specimen
5A E13 WT:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 13.5
|
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
|
Sex:
|
Male
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
|
Note:
|
late of the day (16-17)
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS21: mammary mesenchyme |
Present |
Regionally restricted |
5A E13 WT
|
Sensory neuron innervations were seen. Decreased innervation (about 50%) compared with that of E12 male (quantified in Fig 5B).
|
| TS21: spinal nerve |
Present |
Regionally restricted |
5A E13 WT
|
Sensory neuron innervations were seen. Decreased innervation (about 50%) compared with that of E12 male (quantified in Fig 5B).
|
Specimen
5A E13 KO:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd
|
|
Mutant Allele(s):
|
Plxna4tm1Matl/Plxna4tm1Matl
|
|
Age:
|
embryonic day 13.5
|
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
|
Sex:
|
Male
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
|
Note:
|
late of the day (16-17)
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS21: mammary mesenchyme |
Present |
Regionally restricted |
5A E13 KO
|
Highly increased sensory neuron innervations, compared with the wild-type male (quantified in Fig 5B). No decrease in fiber density between late E12 and late E13 in the mutant.
|
| TS21: spinal nerve |
Present |
Regionally restricted |
5A E13 KO
|
Highly increased sensory neuron innervations, compared with the wild-type male (quantified in Fig 5B). No decrease in fiber density between late E12 and late E13 in the mutant.
|
Specimen
5A E13 Het:
embryonic day 13.5; Bdnftm1Jae/Bdnf +
(more )
Note: late of the day (16-17)
Specimen
5A E13 Het:
(close )
|
Genetic Background:
|
involves: 129S4/SvJae
|
|
Mutant Allele(s):
|
Bdnftm1Jae/Bdnf+
|
|
Age:
|
embryonic day 13.5
|
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
|
Sex:
|
Male
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
|
Note:
|
late of the day (16-17)
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS21: mammary mesenchyme |
Present |
Regionally restricted |
5A E13 Het
|
Decreased sensory neuron innervations, compared with the wild-type male (quantified in Fig 5C).
|
| TS21: spinal nerve |
Present |
Regionally restricted |
5A E13 Het
|
Decreased sensory neuron innervations, compared with the wild-type male (quantified in Fig 5C).
|
Specimen
5A E13 KO/Het:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd * 129S4/SvJae
|
|
Mutant Allele(s):
|
Bdnftm1Jae/Bdnf+
Plxna4tm1Matl/Plxna4tm1Matl
|
|
Age:
|
embryonic day 13.5
|
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
|
Sex:
|
Male
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
|
Note:
|
late of the day (16-17)
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS21: mammary mesenchyme |
Present |
Regionally restricted |
5A E13 KO/Het
|
Increased sensory neuron innervations, compared with the wild-type female (quantified in Fig 5D). There was also a decrease (about 50%) on the innervation density of the gland compared to PlexinA4 KO.
|
| TS21: spinal nerve |
Present |
Regionally restricted |
5A E13 KO/Het
|
Increased sensory neuron innervations, compared with the wild-type female (quantified in Fig 5D). There was also a decrease (about 50%) on the innervation density of the gland compared to PlexinA4 KO.
|
Specimen
5A E14 WT:
embryonic day 14.5
(more )
Note: late of the day (16-17)
Specimen
5A E14 WT:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 14.5
|
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
|
Sex:
|
Male
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
|
Note:
|
late of the day (16-17)
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: intercostal nerve |
Present |
Regionally restricted |
5A E14 WT
|
Sensory neuron innervations were seen. Decreased innervation compared with that of E12 male (quantified in Fig 5B).
|
| TS22: mammary mesenchyme |
Present |
Regionally restricted |
5A E14 WT
|
Sensory neuron innervations were seen. Decreased innervation compared with that of E12 male (quantified in Fig 5B).
|
Specimen
5A E14 KO:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd
|
|
Mutant Allele(s):
|
Plxna4tm1Matl/Plxna4tm1Matl
|
|
Age:
|
embryonic day 14.5
|
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
|
Sex:
|
Male
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
|
Note:
|
late of the day (16-17)
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: intercostal nerve |
Present |
Regionally restricted |
5A E14 KO
|
A sharp decrease in fiber density compared with that of the E13 mutant, but to a level which was still significantly higher than that of the wild-type male (quantified in Fig 5B).
|
| TS22: mammary mesenchyme |
Present |
Regionally restricted |
5A E14 KO
|
A sharp decrease in fiber density compared with that of the E13 mutant, but to a level which was still significantly higher than that of the wild-type male (quantified in Fig 5B).
|
Specimen
5A E14 Het:
embryonic day 14.5; Bdnftm1Jae/Bdnf +
(more )
Note: late of the day (16-17)
Specimen
5A E14 Het:
(close )
|
Genetic Background:
|
involves: 129S4/SvJae
|
|
Mutant Allele(s):
|
Bdnftm1Jae/Bdnf+
|
|
Age:
|
embryonic day 14.5
|
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
|
Sex:
|
Male
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
|
Note:
|
late of the day (16-17)
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: intercostal nerve |
Present |
Regionally restricted |
5A E14 Het
|
Decreased sensory neuron innervations, compared with the wild-type male (quantified in Fig 5C).
|
| TS22: mammary mesenchyme |
Present |
Regionally restricted |
5A E14 Het
|
Decreased sensory neuron innervations, compared with the wild-type male (quantified in Fig 5C).
|
Specimen
5A E14 KO/Het:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd * 129S4/SvJae
|
|
Mutant Allele(s):
|
Bdnftm1Jae/Bdnf+
Plxna4tm1Matl/Plxna4tm1Matl
|
|
Age:
|
embryonic day 14.5
|
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
|
Sex:
|
Male
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
|
Note:
|
late of the day (16-17)
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: intercostal nerve |
Present |
Regionally restricted |
5A E14 KO/Het
|
Increased sensory neuron innervations, compared with the wild-type female (quantified in Fig 5D). The innervation density of the gland was similar as the PlexinA4 KO.
|
| TS22: mammary mesenchyme |
Present |
Regionally restricted |
5A E14 KO/Het
|
Increased sensory neuron innervations, compared with the wild-type female (quantified in Fig 5D). The innervation density of the gland was similar as the PlexinA4 KO.
|
Specimen
7A E13 WT:
embryonic day 13.5
(more )
Note: late of the day (16-17)
Specimen
7A E13 WT:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 13.5
|
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
|
Sex:
|
Male
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
|
Note:
|
late of the day (16-17)
|
Specimen
7A E13 KO1:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd
|
|
Mutant Allele(s):
|
Plxna4tm1Matl/Plxna4tm1Matl
|
|
Age:
|
embryonic day 13.5
|
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
|
Sex:
|
Male
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
|
Note:
|
late of the day (16-17)
|
Specimen
7A E13 KO2:
(close )
|
Genetic Background:
|
involves: 129S1/Sv
|
|
Mutant Allele(s):
|
Ntrk2tm1.1Tes/Ntrk2tm1.1Tes
|
|
Age:
|
embryonic day 13.5
|
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
|
Sex:
|
Male
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
|
Note:
|
late of the day (16-17)
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS21: mammary mesenchyme |
Present |
Regionally restricted |
7A E13 KO-2
|
Increased sensory neuron innervations, compared with the wild-type male. Decreased innervation compared with that of E13 PlexinA4 KO.
|
| TS21: spinal nerve |
Present |
Regionally restricted |
7A E13 KO-2
|
Increased sensory neuron innervations, compared with the wild-type male. Decreased innervation compared with that of E13 PlexinA4 KO.
|
Specimen
7A E13 KO1/KO2:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd * 129S1/Sv
|
|
Mutant Allele(s):
|
Ntrk2tm1.1Tes/Ntrk2tm1.1Tes
Plxna4tm1Matl/Plxna4tm1Matl
|
|
Age:
|
embryonic day 13.5
|
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
|
Sex:
|
Male
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
|
Note:
|
late of the day (16-17)
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS21: mammary mesenchyme |
Present |
Regionally restricted |
7A E13 KO-1/KO-2
|
Increased sensory neuron innervations, compared with the wild-type male. Similar innervation compared with that of E13 PlexinA4 KO.
|
| TS21: spinal nerve |
Present |
Regionally restricted |
7A E13 KO-1/KO-2
|
Increased sensory neuron innervations, compared with the wild-type male. Similar innervation compared with that of E13 PlexinA4 KO.
|
Specimen
7A E14 WT:
embryonic day 14.5
(more )
Note: late of the day (16-17)
Specimen
7A E14 WT:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 14.5
|
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
|
Sex:
|
Male
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
|
Note:
|
late of the day (16-17)
|
Specimen
7A E14 KO1:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd
|
|
Mutant Allele(s):
|
Plxna4tm1Matl/Plxna4tm1Matl
|
|
Age:
|
embryonic day 14.5
|
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
|
Sex:
|
Male
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
|
Note:
|
late of the day (16-17)
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: intercostal nerve |
Present |
Regionally restricted |
7A E14 KO-1
|
Increased sensory neuron innervations, compared with the wild-type male. Decreased innervation compared with that of E13 PlexinA4 KO.
|
| TS22: mammary mesenchyme |
Present |
Regionally restricted |
7A E14 KO-1
|
Increased sensory neuron innervations, compared with the wild-type male. Decreased innervation compared with that of E13 PlexinA4 KO.
|
Specimen
7A E14 KO2:
(close )
|
Genetic Background:
|
involves: 129S1/Sv
|
|
Mutant Allele(s):
|
Ntrk2tm1.1Tes/Ntrk2tm1.1Tes
|
|
Age:
|
embryonic day 14.5
|
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
|
Sex:
|
Male
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
|
Note:
|
late of the day (16-17)
|
Specimen
7A E14 KO1/KO2:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd * 129S1/Sv
|
|
Mutant Allele(s):
|
Ntrk2tm1.1Tes/Ntrk2tm1.1Tes
Plxna4tm1Matl/Plxna4tm1Matl
|
|
Age:
|
embryonic day 14.5
|
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
|
Sex:
|
Male
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
|
Note:
|
late of the day (16-17)
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: intercostal nerve |
Present |
Regionally restricted |
7A E14 KO-1/KO-2
|
Increased sensory neuron innervations, compared with the wild-type male. Similar innervation compared with that of E13 PlexinA4 KO.
|
| TS22: mammary mesenchyme |
Present |
Regionally restricted |
7A E14 KO-1/KO-2
|
Increased sensory neuron innervations, compared with the wild-type male. Similar innervation compared with that of E13 PlexinA4 KO.
|
|
Analysis Tools