Ntrk2 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:277791 Sar Shalom H, et al., Balance between BDNF and Semaphorins gates the innervation of the mammary gland. Elife. 2019 Jan 10;8:e41162
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:7579214
Gene symbol:	Ntrk2
Gene name:	neurotrophic tyrosine kinase, receptor, type 2

Antibody:	Anti-mouse TrkB (AF1494)
Detection system:	Secondary antibody coupled to Cy3

Results

1A ©

Specimen 1A: embryonic day 13.5 (more

)
Note: Late of the day (16-17).

Structure	Level	Pattern	Image	Note
TS21: spinal nerve	Present	Regionally restricted	1A	Expressed in sensory axons innervating the mammary gland.

1B ©

Specimen 1B: embryonic day 13.5 (more

)
Note: Late of the day (16-17).

Structure	Level	Pattern	Image	Note
TS21: spinal nerve	Present	Regionally restricted	1B	Reduced innervation to the mammary gland compared with the female.
TS21: mammary mesenchyme	Present	Regionally restricted	1B	Expressed in the mesenchymal cells surrounding the mammary epithelium.

4-S1_WT TrkB ©

Specimen 4-S1 WT: embryonic day 13.5 (more

)
Note: Late of the day (16-17). Double labeled: red - Ntrk2; green - Tubb3 (assay MGI:7579233).

Structure	Level	Pattern	Image	Note
TS21: mammary mesenchyme	Present	Regionally restricted	4-S1_WT TrkB , 4-S1_WT merge	Sensory neuron innervations (coexpressed with Tubb3) were seen.
TS21: spinal nerve	Present	Regionally restricted	4-S1_WT TrkB , 4-S1_WT merge	Sensory neuron innervations (coexpressed with Tubb3) were seen.

4-S1_KO TrkB ©

Specimen 4-S1 KO: embryonic day 13.5; Plxna4^tm1Matl/Plxna4^tm1Matl (more

)
Note: Late of the day (16-17). Double labeled: red - Ntrk2; green - Tubb3 (assay MGI:7579233).

Structure	Level	Pattern	Image	Note
TS21: mammary mesenchyme	Present	Regionally restricted	4-S1_KO merge , 4-S1_KO TrkB	Increased sensory neuron innervations (coexpressed with Tubb3), compared with the wild-type female.
TS21: spinal nerve	Present	Regionally restricted	4-S1_KO merge , 4-S1_KO TrkB	Increased sensory neuron innervations (coexpressed with Tubb3), compared with the wild-type female.

5-S1_WT TrkB ©

Specimen 5-S1 WT: embryonic day 13.5 (more

)
Note: Late of the day (16-17). Double labeled: red - Ntrk2; green - Tubb3 (assay MGI:7579233).

Structure	Level	Pattern	Image	Note
TS21: mammary mesenchyme	Present	Regionally restricted	5-S1_WT TrkB , 5-S1_WT merge	Sensory neuron innervations (coexpressed with Tubb3) were seen.
TS21: spinal nerve	Present	Regionally restricted	5-S1_WT TrkB , 5-S1_WT merge	Sensory neuron innervations (coexpressed with Tubb3) were seen.

5-S1_KO TrkB ©

Specimen 5-S1 KO: embryonic day 13.5; Plxna4^tm1Matl/Plxna4^tm1Matl (more

)
Note: Late of the day (16-17). Double labeled: red - Ntrk2; green - Tubb3 (assay MGI:7579233).

Structure	Level	Pattern	Image	Note
TS21: mammary mesenchyme	Present	Regionally restricted	5-S1_KO merge , 5-S1_KO TrkB	Increased sensory neuron innervations (coexpressed with Tubb3), compared with the wild-type male.
TS21: spinal nerve	Present	Regionally restricted	5-S1_KO merge , 5-S1_KO TrkB	Increased sensory neuron innervations (coexpressed with Tubb3), compared with the wild-type male.

5-S2_WT ©

Specimen 5-S2 WT: embryonic day 13.5 (more

)
Note: Late of the day (16-17).

Structure	Level	Pattern	Image	Note
TS21: mammary mesenchyme	Present	Regionally restricted	5-S2_WT	Expressed in sensory neuron innervations and mesenchymal cells surrounding the gland.
TS21: spinal nerve	Present	Regionally restricted	5-S2_WT	Expressed in sensory neuron innervations.

5-S2_KO ©

Specimen 5-S2 KO: embryonic day 13.5; Plxna4^tm1Matl/Plxna4^tm1Matl (more

)
Note: Late of the day (16-17).

Structure	Level	Pattern	Image	Note
TS21: spinal nerve	Present	Regionally restricted	5-S2_KO	Increased sensory neuron innervations, compared with the wild-type male.
TS21: mammary mesenchyme	Present	Regionally restricted	5-S2_KO	Increased sensory neuron innervations, compared with the wild-type male. Expression in the mesenchymal cells surrounding the gland was not changed.

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