Ntrk2 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:277791 Sar Shalom H, et al., Balance between BDNF and Semaphorins gates the innervation of the mammary gland. Elife. 2019 Jan 10;8:e41162
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:7579214
Gene symbol:	Ntrk2
Gene name:	neurotrophic tyrosine kinase, receptor, type 2

Antibody:	Anti-mouse TrkB (AF1494)
Detection system:	Secondary antibody coupled to Cy3

Results

1A ©

Specimen 1A: embryonic day 13.5 (more

)
Note: Late of the day (16-17).

Structure	Level	Pattern	Image	Note
TS21: spinal nerve	Present	Regionally restricted	1A	Expressed in sensory axons innervating the mammary gland.

1B ©

Specimen 1B: embryonic day 13.5 (more

)
Note: Late of the day (16-17).

Structure	Level	Pattern	Image	Note
TS21: spinal nerve	Present	Regionally restricted	1B	Reduced innervation to the mammary gland compared with the female.
TS21: mammary mesenchyme	Present	Regionally restricted	1B	Expressed in the mesenchymal cells surrounding the mammary epithelium.

4-S1_WT TrkB ©

Specimen 4-S1 WT: embryonic day 13.5 (more

)
Note: Late of the day (16-17). Double labeled: red - Ntrk2; green - Tubb3 (assay MGI:7579233).

Structure	Level	Pattern	Image	Note
TS21: mammary mesenchyme	Present	Regionally restricted	4-S1_WT TrkB , 4-S1_WT merge	Sensory neuron innervations (coexpressed with Tubb3) were seen.
TS21: spinal nerve	Present	Regionally restricted	4-S1_WT TrkB , 4-S1_WT merge	Sensory neuron innervations (coexpressed with Tubb3) were seen.

4-S1_KO TrkB ©

Specimen 4-S1 KO: embryonic day 13.5; Plxna4^tm1Matl/Plxna4^tm1Matl (more

)
Note: Late of the day (16-17). Double labeled: red - Ntrk2; green - Tubb3 (assay MGI:7579233).

Structure	Level	Pattern	Image	Note
TS21: mammary mesenchyme	Present	Regionally restricted	4-S1_KO merge , 4-S1_KO TrkB	Increased sensory neuron innervations (coexpressed with Tubb3), compared with the wild-type female.
TS21: spinal nerve	Present	Regionally restricted	4-S1_KO merge , 4-S1_KO TrkB	Increased sensory neuron innervations (coexpressed with Tubb3), compared with the wild-type female.

5-S1_WT TrkB ©

Specimen 5-S1 WT: embryonic day 13.5 (more

)
Note: Late of the day (16-17). Double labeled: red - Ntrk2; green - Tubb3 (assay MGI:7579233).

Structure	Level	Pattern	Image	Note
TS21: mammary mesenchyme	Present	Regionally restricted	5-S1_WT TrkB , 5-S1_WT merge	Sensory neuron innervations (coexpressed with Tubb3) were seen.
TS21: spinal nerve	Present	Regionally restricted	5-S1_WT TrkB , 5-S1_WT merge	Sensory neuron innervations (coexpressed with Tubb3) were seen.

5-S1_KO TrkB ©

Specimen 5-S1 KO: embryonic day 13.5; Plxna4^tm1Matl/Plxna4^tm1Matl (more

)
Note: Late of the day (16-17). Double labeled: red - Ntrk2; green - Tubb3 (assay MGI:7579233).

Structure	Level	Pattern	Image	Note
TS21: mammary mesenchyme	Present	Regionally restricted	5-S1_KO merge , 5-S1_KO TrkB	Increased sensory neuron innervations (coexpressed with Tubb3), compared with the wild-type male.
TS21: spinal nerve	Present	Regionally restricted	5-S1_KO merge , 5-S1_KO TrkB	Increased sensory neuron innervations (coexpressed with Tubb3), compared with the wild-type male.

5-S2_WT ©

Specimen 5-S2 WT: embryonic day 13.5 (more

)
Note: Late of the day (16-17).

Structure	Level	Pattern	Image	Note
TS21: mammary mesenchyme	Present	Regionally restricted	5-S2_WT	Expressed in sensory neuron innervations and mesenchymal cells surrounding the gland.
TS21: spinal nerve	Present	Regionally restricted	5-S2_WT	Expressed in sensory neuron innervations.

5-S2_KO ©

Specimen 5-S2 KO: embryonic day 13.5; Plxna4^tm1Matl/Plxna4^tm1Matl (more

)
Note: Late of the day (16-17).

Structure	Level	Pattern	Image	Note
TS21: spinal nerve	Present	Regionally restricted	5-S2_KO	Increased sensory neuron innervations, compared with the wild-type male.
TS21: mammary mesenchyme	Present	Regionally restricted	5-S2_KO	Increased sensory neuron innervations, compared with the wild-type male. Expression in the mesenchymal cells surrounding the gland was not changed.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources Funding Information Warranty Disclaimer, Privacy Notice, Licensing, & Copyright Send questions and comments to User Support.	last database update 05/14/2024 MGI 6.23