Antibody:	anti-AP-2gamma (6E4/4) (sc-12762)
Detection system:	Secondary antibody coupled to Alexa Fluor 488

Structure	Level	Pattern	Image	Note
TS20: retina	Absent

Structure	Level	Pattern	Image	Note
TS23: retina layer	Present	Scattered	3A	Expression was scattered in the inner and outer neuroblast layers.

Structure	Level	Pattern	Image	Note
TS27: retina inner nuclear layer	Present	Regionally restricted	3B	Expression was detected in the neuroblast layer in the inner nuclear layer.
TS27: retina ganglion cell layer	Present	Regionally restricted	3B	Immunopositive cells were found infrequently in the ganglion cell layer.

Structure	Level	Pattern	Image	Note
TS28: retina inner nuclear layer	Present		3C
TS28: retina ganglion cell layer	Present	Single cells	3C	Very few labeled cells were observed in the ganglion cell layer.

Structure	Level	Pattern	Image	Note
TS28: retina inner nuclear layer	Present		3D
TS28: retina ganglion cell layer	Present	Single cells	3D	Very few labeled cells were observed in the ganglion cell layer.

Structure	Cell Type	Level	Pattern	Image	Note
TS24: neural retina	retina horizontal cell	Absent		3E	No co-expression with the horizontal cell marker Prox1 was observed.

Structure	Cell Type	Level	Pattern	Image	Note
TS27: neural retina	retina horizontal cell	Trace	Single cells	3F	Only 0.92% of Uchl1-positive cells expressed Tfap2c.

Structure	Level	Pattern	Image	Note
TS23: retina	Present		4A	No co-labeling with PH3 was observed.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: neural retina	GABAergic amacrine cell	Present		4B	Expression co-localized with Slc6a1.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: neural retina	glycinergic amacrine cell	Present		4C	Expression co-localized with Slc6a9.

Structure	Cell Type	Level	Pattern	Image	Note
TS27: neural retina		Present	Regionally restricted	4E	Staining overlapped with Pax6-positive cells in the neuroblast layer.
TS27: retina inner nuclear layer	amacrine cell	Present		4E	Staining overlapped with Pax6-positive cells.

Structure	Level	Pattern	Image	Note
TS28: neural retina	Present	Regionally restricted	4F	Staining overlapped with Pax6-positive cells.

Structure	Level	Pattern	Image	Note
TS28: neural retina	Present	Regionally restricted	4G	Staining overlapped with Pax6-positive cells.

Structure	Cell Type	Level	Pattern	Image	Note
TS27: neural retina	GABAergic amacrine cell	Present		4H	Expression co-localized with Bhlhe22.

Structure	Level	Pattern	Image	Note
TS27: neural retina	Present		4K	Expression was not co-localized with Sox2 in cholinergic amacrine cells.

Structure	Level	Pattern	Image	Note
TS24: neural retina	Present	Regionally restricted	5A	Expression was detected both singly and co-labeled with Tfap2b. The Tfap2c-positive population was smaller than the Tfap2b-positive population. Approximately 20% of the immunopositive population was double labeled.

Structure	Level	Pattern	Image	Note
TS27: neural retina	Present	Regionally restricted	5B	Expression was detected both singly and co-labeled with Tfap2b. The Tfap2c-positive population was smaller than the Tfap2b-positive population. Approximately 20% of the immunopositive population was double labeled.

Structure	Level	Pattern	Image	Note
TS28: neural retina	Present	Regionally restricted	5C	Expression was detected both singly and co-labeled with Tfap2b. The Tfap2c-positive population was smaller than the Tfap2b-positive population.

Structure	Level	Pattern	Image	Note
TS28: neural retina	Present		5D	Expression was detected both singly and co-labeled with Tfap2b. The Tfap2c-positive population was smaller than the Tfap2b-positive population. Only 3.4% of the immunopositive population was double labeled in the mature retina.

Structure	Level	Pattern	Image	Note
TS24: retina	Present			The vast majority (>98%) of Tfap2c immunopositive cells did not co-express Ki67.

Structure	Cell Type	Level	Pattern	Image	Note
TS27: photoreceptor layer	photoreceptor cell	Absent			Tfap2c did not co-label photoreceptor cells with Otx2 or Crx.
TS27: retina	cone retinal bipolar cell	Absent			Tfap2c did not co-label bipolar cells with Vsx2.
TS27: retina	Mueller cell	Absent			Tfap2c did not co-label Muller glial cells with Sox2.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: photoreceptor layer	photoreceptor cell	Absent			Tfap2c did not co-label photoreceptor cells with Otx2 or Crx.
TS28: retina	cone retinal bipolar cell	Absent			Tfap2c did not co-label bipolar cells with Vsx2.
TS28: retina	Mueller cell	Absent			Tfap2c did not co-label Muller glial cells with Sox2.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: photoreceptor layer	photoreceptor cell	Absent			Tfap2c did not co-label photoreceptor cells with Otx2 or Crx.
TS28: retina	cone retinal bipolar cell	Absent			Tfap2c did not co-label bipolar cells with Vsx2.
TS28: retina	Mueller cell	Absent			Tfap2c did not co-label Muller glial cells with Sox2.