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Gene Expression Data
Assay Details
Assay
Reference: J:181601 Bassett EA, et al., Overlapping expression patterns and redundant roles for AP-2 transcription factors in the developing mammalian retina. Dev Dyn. 2012 Apr;241(4):814-29
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7578959
Gene symbol: Tfap2a
Gene name: transcription factor AP-2, alpha
Antibody: clone 3B5
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen 1A: embryonic day 15.5 (more )
Note: Double labeled: green - Tfap2a; red - Tfap2b (assay MGI:7578990).
Structure Level Pattern Image Note
TS23: retina Present 1A Extensive co-localized immunostaining with Tfap2b was detected.
Specimen 2D: postnatal day 0 (more )
Note: Double labeled: green - Tfap2a; red - Tfap2b (assay MGI:7578990).
Structure Cell Type Level Pattern Image Note
TS27: neural retina retina horizontal cell Present Regionally restricted 2D Extensive overlap between Tfap2a and Tfap2b expression was observed (80% of immunopositive cells). In the outer retina, putative horizontal cells exhibiting strong Tfap2b signal were weakly stained for Tfap2a.
Specimen 2E: embryonic day 16.5 (more )
Note: Double labeled: green - Tfap2a; red - Prox1 (assay MGI:7578999).
Structure Cell Type Level Pattern Image Note
TS24: neural retina retina horizontal cell Present 2E Expression was detected in horizontal cells that co-express Prox1.
Specimen 2F: postnatal day 0 (more )
Note: Double labeled: green - Tfap2a; red - Uchl1 (assay MGI:7579001).
Structure Cell Type Level Pattern Image Note
TS27: neural retina retina horizontal cell Present 2F Expression was detected in horizontal cells that co-express Uchl1 (94% of Uchl1-positive cells expressed Tfap2a).
Specimen 4I: postnatal day 0 (more )
Note: Double labeled: green - Tfap2a; red - Bhlhe22 (assay MGI:7579011).
Structure Cell Type Level Pattern Image Note
TS27: neural retina GABAergic amacrine cell Present 4I Expression co-localized with Bhlhe22.
Specimen 4L: postnatal day 0 (more )
Note: Double labeled: green - Tfap2a; red - Sox2 (assay MGI:7579012).
Structure Cell Type Level Pattern Image Note
TS27: neural retina amacrine cell Present Regionally restricted 4L Expression co-localized with Sox2 in cholinergic amacrine cells.
Specimen S2B Wild-Type: embryonic day 18.5 (more )
Structure Level Pattern Image Note
TS26: retina Present Regionally restricted S2B Wild-Type AP-2a A staining domain was detected in central and peripheral retina.
Specimen S2B Tcfap2a/b Mutant: embryonic day 18.5; Tfap2atm1Hsv/Tfap2atm1.1Hsv, Tfap2btm1Rbu/Tfap2btm1Rbu, Tg(Pax6-cre,GFP)2Pgr/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS26: retina Present Regionally restricted S2B Tcfap2a/b Mutant AP-2a The mutant retina demonstrated a loss of staining in the peripheral region.
Specimen S3A: postnatal day 0; Tfap2atm1Hsv/Tfap2atm1.1Hsv, Tfap2btm1Rbu/Tfap2btm1Rbu, Tg(Pax6-cre,GFP)2Pgr/0 (more )
Note: Conditional mutant. Double labeled: green - Tfap2a; red - Uchl1 (assay MGI:7579001).
Structure Cell Type Level Pattern Image Note
TS27: neural retina retina horizontal cell Present Regionally restricted S3A Expression was detected in central retina, where the Cre activity diminishes.
Specimen not shown: embryonic day 16.5 (more )
Note: Data from Fig. S1A.
Structure Level Pattern Image Note
TS24: retina Present The vast majority (>98%) of Tfap2a immunopositive cells did not co-express Ki67.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/23/2024
MGI 6.23 		The Jackson Laboratory