Tph2 RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:316482 Kouwenhoven WM, et al., Nkx2.9 Contributes to Mid-Hindbrain Patterning by Regulation of mdDA Neuronal Cell-Fate and Repression of a Hindbrain-Specific Cell-Fate. Int J Mol Sci. 2021 Nov 23;22(23)
Assay type:	RNA in situ
MGI Accession ID:	MGI:7576059
Gene symbol:	Tph2
Gene name:	tryptophan hydroxylase 2

Probe:	Tph2 cDNA
Probe preparation:	Antisense labelled with digoxigenin RNA

Results

7E +/+ left ©

Specimen 7E +/+ lateral to medial 1: embryonic day 14.5 (more

)

Structure	Level	Pattern	Image	Note
TS22: hindbrain	Present		7E +/+ left

Specimen 7E +/+ lateral to medial 2: embryonic day 14.5 (more

)

Structure	Level	Pattern	Image	Note
TS22: hindbrain	Present		7E +/+ middle

7E +/+ right ©

Specimen 7E +/+ lateral to medial 3: embryonic day 14.5 (more

)

Structure	Level	Pattern	Image	Note
TS22: hindbrain	Present		7E +/+ right

Specimen 7E LacZ/LacZ lateral to medial 1: embryonic day 14.5; Nkx2-9^tm1Jlck/Nkx2-9^tm1Jlck (more

)

Structure	Level	Pattern	Image	Note
TS22: hindbrain	Present		7E LacZ/LacZ left	The expression area extended more rostrally to the caudal part of the midbrain.

Specimen 7E LacZ/LacZ lateral to medial 2: embryonic day 14.5; Nkx2-9^tm1Jlck/Nkx2-9^tm1Jlck (more

)

Structure	Level	Pattern	Image	Note
TS22: hindbrain	Present		7E LacZ/LacZ middle	The expression area extended more rostrally to the caudal part of the midbrain.

Specimen 7E LacZ/LacZ lateral to medial 3: embryonic day 14.5; Nkx2-9^tm1Jlck/Nkx2-9^tm1Jlck (more

)

Structure	Level	Pattern	Image	Note
TS22: hindbrain	Present		7E LacZ/LacZ right	The expression area extended more rostrally to the caudal part of the midbrain.

Specimen 7E' +/+: embryonic day 14.5 (more

)
Note: Pseudo-overlay: red - Tph2; green - Th (assay MGI:7576032).

Structure	Level	Pattern	Image	Note
TS22: hindbrain	Present		7E' upper right , 7E' upper left	A strong separation between the Th and Tph2 expression areas was seen.

Specimen 7E' LacZ/LacZ: embryonic day 14.5; Nkx2-9^tm1Jlck/Nkx2-9^tm1Jlck (more

)
Note: Pseudo-overlay: red - Tph2; green - Th (assay MGI:7576032).

Structure	Level	Pattern	Image	Note
TS22: hindbrain	Present		7E' lower left , 7E' lower right	The expression area extended more rostrally to the caudal part of the midbrain. The strong separation of the two expression areas was lost in the isthmic area.
TS22: midbrain-hindbrain junction	Present		7E' lower right , 7E' lower left	The expression area extended more rostrally to the caudal part of the midbrain, even intruding the caudal Th expression area.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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