Th RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:316482 Kouwenhoven WM, et al., Nkx2.9 Contributes to Mid-Hindbrain Patterning by Regulation of mdDA Neuronal Cell-Fate and Repression of a Hindbrain-Specific Cell-Fate. Int J Mol Sci. 2021 Nov 23;22(23)
Assay type:	RNA in situ
MGI Accession ID:	MGI:7576032
Gene symbol:	Th
Gene name:	tyrosine hydroxylase

Probe:	Th probe
Probe preparation:	Antisense labelled with digoxigenin RNA

Results

5C +/+ 1 ©

Specimen 5C +/+ lateral to medial 1: embryonic day 14.5 (more

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Structure	Cell Type	Level	Pattern	Image	Note
TS22: midbrain	midbrain dopaminergic neuron	Present		5C +/+ 1	Expressed in mesodiencephalic dopaminergic neurons.

5C +/+ 2 ©

Specimen 5C +/+ lateral to medial 2: embryonic day 14.5 (more

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Structure	Cell Type	Level	Pattern	Image	Note
TS22: midbrain	midbrain dopaminergic neuron	Present		5C +/+ 2	Expressed in mesodiencephalic dopaminergic neurons.

5C +/+ 3 ©

Specimen 5C +/+ lateral to medial 3: embryonic day 14.5 (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS22: midbrain	midbrain dopaminergic neuron	Present		5C +/+ 3	Expressed in mesodiencephalic dopaminergic neurons.

5C +/+ 4 ©

Specimen 5C +/+ lateral to medial 4: embryonic day 14.5 (more

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Structure	Cell Type	Level	Pattern	Image	Note
TS22: midbrain	midbrain dopaminergic neuron	Present		5C +/+ 4	Expressed in mesodiencephalic dopaminergic neurons.

5C +/+ 5 ©

Specimen 5C +/+ lateral to medial 5: embryonic day 14.5 (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS22: midbrain	midbrain dopaminergic neuron	Present		5C +/+ 5	Expressed in mesodiencephalic dopaminergic neurons.

5C LacZ/LacZ 1 ©

Specimen 5C LacZ/LacZ lateral to medial 1: embryonic day 14.5; Nkx2-9^tm1Jlck/Nkx2-9^tm1Jlck (more

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Structure	Cell Type	Level	Pattern	Image	Note
TS22: midbrain	midbrain dopaminergic neuron	Present		5C LacZ/LacZ 1	Similar as in the wild-type.

5C LacZ/LacZ 2 ©

Specimen 5C LacZ/LacZ lateral to medial 2: embryonic day 14.5; Nkx2-9^tm1Jlck/Nkx2-9^tm1Jlck (more

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Structure	Cell Type	Level	Pattern	Image	Note
TS22: midbrain	midbrain dopaminergic neuron	Present		5C LacZ/LacZ 2	Similar as in the wild-type.

5C LacZ/LacZ 3 ©

Specimen 5C LacZ/LacZ lateral to medial 3: embryonic day 14.5; Nkx2-9^tm1Jlck/Nkx2-9^tm1Jlck (more

)

Structure	Level	Pattern	Image	Note
TS22: midbrain	Present		5C LacZ/LacZ 3	Similar as in the wild-type.

5C LacZ/LacZ 4 ©

Specimen 5C LacZ/LacZ lateral to medial 4: embryonic day 14.5; Nkx2-9^tm1Jlck/Nkx2-9^tm1Jlck (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS22: midbrain	midbrain dopaminergic neuron	Present		5C LacZ/LacZ 4	Similar as in the wild-type.

5C LacZ/LacZ 5 ©

Specimen 5C LacZ/LacZ lateral to medial 5: embryonic day 14.5; Nkx2-9^tm1Jlck/Nkx2-9^tm1Jlck (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS22: midbrain	midbrain dopaminergic neuron	Present		5C LacZ/LacZ 5	Similar as in the wild-type.

7E' upper left ©

7E' upper right ©

Specimen 7E' +/+: embryonic day 14.5 (more

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Note: Pseudo-overlay: green - Th; red - Tph2 (assay MGI:7576059).

Structure	Cell Type	Level	Pattern	Image	Note
TS22: midbrain	midbrain dopaminergic neuron	Present		7E' upper left , 7E' upper right

7E' lower left ©

7E' lower right ©

Specimen 7E' LacZ/LacZ: embryonic day 14.5; Nkx2-9^tm1Jlck/Nkx2-9^tm1Jlck (more

)
Note: Pseudo-overlay: green - Th; red - Tph2 (assay MGI:7576059).

Structure	Cell Type	Level	Pattern	Image	Note
TS22: midbrain	midbrain dopaminergic neuron	Present		7E' lower right , 7E' lower left

S1B WT 1 ©

Specimen S1B WT rostral to caudal 1: postnatal adult (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS28: midbrain	midbrain dopaminergic neuron	Present		S1B WT 1

S1B WT 2 ©

Specimen S1B WT rostral to caudal 2: postnatal adult (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS28: midbrain	midbrain dopaminergic neuron	Present		S1B WT 2

S1B WT 3 ©

Specimen S1B WT rostral to caudal 3: postnatal adult (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS28: midbrain	midbrain dopaminergic neuron	Present		S1B WT 3

S1B WT 4 ©

Specimen S1B WT rostral to caudal 4: postnatal adult (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS28: midbrain	midbrain dopaminergic neuron	Present		S1B WT 4

S1B KO 1 ©

Specimen S1B KO rostral to caudal 1: postnatal adult; Nkx2-9^tm1Jlck/Nkx2-9^tm1Jlck (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS28: midbrain	midbrain dopaminergic neuron	Present		S1B KO 1	Similar as in the wild-type.

S1B KO 2 ©

Specimen S1B KO rostral to caudal 2: postnatal adult; Nkx2-9^tm1Jlck/Nkx2-9^tm1Jlck (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS28: midbrain	midbrain dopaminergic neuron	Present		S1B KO 2	Similar as in the wild-type.

S1B KO 3 ©

Specimen S1B KO rostral to caudal 3: postnatal adult; Nkx2-9^tm1Jlck/Nkx2-9^tm1Jlck (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS28: midbrain	midbrain dopaminergic neuron	Present		S1B KO 3	Similar as in the wild-type.

S1B KO 4 ©

Specimen S1B KO rostral to caudal 4: postnatal adult; Nkx2-9^tm1Jlck/Nkx2-9^tm1Jlck (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS28: midbrain	midbrain dopaminergic neuron	Present		S1B KO 4	Similar as in the wild-type.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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