Gene Expression Data
Assay Details
Assay
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Reference:
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J:316482
Kouwenhoven WM, et al., Nkx2.9 Contributes to Mid-Hindbrain Patterning by Regulation of mdDA Neuronal Cell-Fate and Repression of a Hindbrain-Specific Cell-Fate. Int J Mol Sci. 2021 Nov 23;22(23)
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7576029
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Gene symbol:
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Th
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Gene name:
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tyrosine hydroxylase
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Antibody:
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anti-TH
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Detection system:
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Secondary antibody coupled to Alexa Fluor 488
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Results
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Specimen
3F lateral to medial 1:
embryonic day 14.5; Nkx2-9tm1Jlck/Nkx2-9 +
(more )
Note: Double labeled: green - Th; red - Nkx2-9 reporter (assay MGI:7576026).
Specimen
3F lateral to medial 1:
(close )
Genetic Background:
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involves: 129/Sv * C57BL/6 * C57BL/6J * SJL
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Mutant Allele(s):
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Nkx2-9tm1Jlck/Nkx2-9+
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Th; red - Nkx2-9 reporter (assay MGI:7576026).
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Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS22: midbrain |
midbrain dopaminergic neuron |
Present |
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3F left
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Expressed in mesodiencephalic dopaminergic neurons.
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Specimen
3F lateral to medial 2:
embryonic day 14.5; Nkx2-9tm1Jlck/Nkx2-9 +
(more )
Note: Double labeled: green - Th; red - Nkx2-9 reporter (assay MGI:7576026).
Specimen
3F lateral to medial 2:
(close )
Genetic Background:
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involves: 129/Sv * C57BL/6 * C57BL/6J * SJL
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Mutant Allele(s):
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Nkx2-9tm1Jlck/Nkx2-9+
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Th; red - Nkx2-9 reporter (assay MGI:7576026).
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Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS22: midbrain |
midbrain dopaminergic neuron |
Present |
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3F middle left
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Expressed in mesodiencephalic dopaminergic neurons.
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Specimen
3F lateral to medial 3:
embryonic day 14.5; Nkx2-9tm1Jlck/Nkx2-9 +
(more )
Note: Double labeled: green - Th; red - Nkx2-9 reporter (assay MGI:7576026).
Specimen
3F lateral to medial 3:
(close )
Genetic Background:
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involves: 129/Sv * C57BL/6 * C57BL/6J * SJL
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Mutant Allele(s):
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Nkx2-9tm1Jlck/Nkx2-9+
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Th; red - Nkx2-9 reporter (assay MGI:7576026).
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS22: midbrain |
midbrain dopaminergic neuron |
Present |
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3F middle right
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Expressed in mesodiencephalic dopaminergic neurons.
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Specimen
3F lateral to medial 4:
embryonic day 14.5; Nkx2-9tm1Jlck/Nkx2-9 +
(more )
Note: Double labeled: green - Th; red - Nkx2-9 reporter (assay MGI:7576026).
Specimen
3F lateral to medial 4:
(close )
Genetic Background:
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involves: 129/Sv * C57BL/6 * C57BL/6J * SJL
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Mutant Allele(s):
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Nkx2-9tm1Jlck/Nkx2-9+
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Th; red - Nkx2-9 reporter (assay MGI:7576026).
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS22: midbrain |
midbrain dopaminergic neuron |
Present |
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3F right
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Expressed in mesodiencephalic dopaminergic neurons.
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Specimen
3H SNc:
postnatal adult; Nkx2-9tm1Jlck/Nkx2-9 +
(more )
Note: Double labeled: green - Th; red - Nkx2-9 reporter (assay MGI:7576026).
Specimen
3H SNc:
(close )
Genetic Background:
|
involves: 129/Sv * C57BL/6 * C57BL/6J * SJL
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Mutant Allele(s):
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Nkx2-9tm1Jlck/Nkx2-9+
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Th; red - Nkx2-9 reporter (assay MGI:7576026).
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Specimen
3H VTA:
postnatal adult; Nkx2-9tm1Jlck/Nkx2-9 +
(more )
Note: Double labeled: green - Th; red - Nkx2-9 reporter (assay MGI:7576026).
Specimen
3H VTA:
(close )
Genetic Background:
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involves: 129/Sv * C57BL/6 * C57BL/6J * SJL
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Mutant Allele(s):
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Nkx2-9tm1Jlck/Nkx2-9+
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Th; red - Nkx2-9 reporter (assay MGI:7576026).
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