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Gene Expression Data
Assay Details
Assay
Reference: J:316482 Kouwenhoven WM, et al., Nkx2.9 Contributes to Mid-Hindbrain Patterning by Regulation of mdDA Neuronal Cell-Fate and Repression of a Hindbrain-Specific Cell-Fate. Int J Mol Sci. 2021 Nov 23;22(23)
Assay type: In situ reporter (knock in)
MGI Accession ID: MGI:7576026
Gene symbol: Nkx2-9
Gene name: NK2 homeobox 9
Reporter: lacZ
Antibody: anti-B-galactosidase
Detection system: Secondary antibody coupled to Alexa Fluor 555
Results
Specimen 3B lateral to medial 1: embryonic day 12.5; Nkx2-9tm1Jlck/Nkx2-9+ (more )
Structure Level Pattern Image Note
TS20: midbrain-hindbrain junction Present Regionally restricted 3B-1 Expressed in the isthmic organizer.
Specimen 3B lateral to medial 2: embryonic day 12.5; Nkx2-9tm1Jlck/Nkx2-9+ (more )
Structure Level Pattern Image Note
TS20: midbrain-hindbrain junction Present Regionally restricted 3B-2 Expressed in the isthmic organizer.
Specimen 3B lateral to medial 3: embryonic day 12.5; Nkx2-9tm1Jlck/Nkx2-9+ (more )
Structure Level Pattern Image Note
TS20: midbrain-hindbrain junction Present Regionally restricted 3B-3 Expressed in the isthmic organizer.
Specimen 3B lateral to medial 4: embryonic day 12.5; Nkx2-9tm1Jlck/Nkx2-9+ (more )
Structure Level Pattern Image Note
TS20: midbrain-hindbrain junction Present Regionally restricted 3B-4 Expressed in the isthmic organizer.
Specimen 3B lateral to medial 5: embryonic day 12.5; Nkx2-9tm1Jlck/Nkx2-9+ (more )
Structure Level Pattern Image Note
TS20: midbrain-hindbrain junction Present Regionally restricted 3B-5 Expressed in the isthmic organizer.
TS20: midbrain Present Regionally restricted 3B-5 Expressed in mesodiencephalic dopaminergic neuron area.
Specimen 3B lateral to medial 6: embryonic day 12.5; Nkx2-9tm1Jlck/Nkx2-9+ (more )
Structure Level Pattern Image Note
TS20: midbrain-hindbrain junction Present Regionally restricted 3B-6 Expressed in the isthmic organizer.
TS20: midbrain Present Regionally restricted 3B-6 Expressed in mesodiencephalic dopaminergic neuron area.
TS20: hindbrain Present 3B-6
Specimen 3D lateral to medial 1: embryonic day 14.5; Nkx2-9tm1Jlck/Nkx2-9+ (more )
Structure Level Pattern Image Note
TS22: midbrain Present Regionally restricted 3D-1 Expressed in mesodiencephalic dopaminergic neuron area. Expressed in the ventral midbrain abutting the mesencephalic flexure.
Specimen 3D lateral to medial 2: embryonic day 14.5; Nkx2-9tm1Jlck/Nkx2-9+ (more )
Structure Level Pattern Image Note
TS22: midbrain Present Regionally restricted 3D-2 Expressed in mesodiencephalic dopaminergic neuron area. Expressed in the ventral midbrain abutting the mesencephalic flexure.
Specimen 3D lateral to medial 3: embryonic day 14.5; Nkx2-9tm1Jlck/Nkx2-9+ (more )
Structure Level Pattern Image Note
TS22: midbrain Present Regionally restricted 3D-3 Expressed in mesodiencephalic dopaminergic neuron area. Expressed in the ventral midbrain abutting the mesencephalic flexure.
Specimen 3D lateral to medial 4: embryonic day 14.5; Nkx2-9tm1Jlck/Nkx2-9+ (more )
Structure Level Pattern Image Note
TS22: midbrain Present Regionally restricted 3D-4 Expressed in mesodiencephalic dopaminergic neuron area. Expressed in the ventral midbrain abutting the mesencephalic flexure.
TS22: hindbrain Present 3D-4
Specimen 3D lateral to medial 5: embryonic day 14.5; Nkx2-9tm1Jlck/Nkx2-9+ (more )
Structure Level Pattern Image Note
TS22: midbrain Present Regionally restricted 3D-5 Expressed in mesodiencephalic dopaminergic neuron area. Expressed in the ventral midbrain abutting the mesencephalic flexure.
TS22: hindbrain Present 3D-5
Specimen 3F lateral to medial 1: embryonic day 14.5; Nkx2-9tm1Jlck/Nkx2-9+ (more )
Note: Double labeled: red - Nkx2-9 reporter; green - Th (assay MGI:7576029).
Structure Cell Type Level Pattern Image Note
TS22: midbrain midbrain dopaminergic neuron Present Regionally restricted 3F left Coexpressed with Th in mesodiencephalic dopaminergic neurons in the rostral-lateral midbrain. Th+beta-gal- neurons were interspersed with double-positive ones.
Specimen 3F lateral to medial 2: embryonic day 14.5; Nkx2-9tm1Jlck/Nkx2-9+ (more )
Note: Double labeled: red - Nkx2-9 reporter; green - Th (assay MGI:7576029).
Structure Cell Type Level Pattern Image Note
TS22: midbrain midbrain dopaminergic neuron Present Regionally restricted 3F middle left Coexpressed with Th in mesodiencephalic dopaminergic neurons in the rostral-lateral midbrain. Th+beta-gal- neurons were interspersed with double-positive ones.
Specimen 3F lateral to medial 3: embryonic day 14.5; Nkx2-9tm1Jlck/Nkx2-9+ (more )
Note: Double labeled: red - Nkx2-9 reporter; green - Th (assay MGI:7576029).
Structure Cell Type Level Pattern Image Note
TS22: midbrain midbrain dopaminergic neuron Present Regionally restricted 3F middle right Coexpressed with Th in mesodiencephalic dopaminergic neurons in the caudomedial midbrain. Th+beta-gal- neurons were interspersed with double-positive ones.
Specimen 3F lateral to medial 4: embryonic day 14.5; Nkx2-9tm1Jlck/Nkx2-9+ (more )
Note: Double labeled: red - Nkx2-9 reporter; green - Th (assay MGI:7576029).
Structure Cell Type Level Pattern Image Note
TS22: midbrain midbrain dopaminergic neuron Present Regionally restricted 3F right Coexpressed with Th in mesodiencephalic dopaminergic neurons in the caudomedial midbrain. Th+beta-gal- neurons were interspersed with double-positive ones.
Specimen 3H SNc: postnatal adult; Nkx2-9tm1Jlck/Nkx2-9+ (more )
Note: Double labeled: red - Nkx2-9 reporter; green - Th (assay MGI:7576029).
Structure Cell Type Level Pattern Image Note
TS28: substantia nigra pars compacta midbrain dopaminergic neuron Present 3H SN Both Th+beta-gal+ and Th+beta-gal- neurons were present.
Specimen 3H VTA: postnatal adult; Nkx2-9tm1Jlck/Nkx2-9+ (more )
Note: Double labeled: red - Nkx2-9 reporter; green - Th (assay MGI:7576029).
Structure Cell Type Level Pattern Image Note
TS28: ventral tegmental area midbrain dopaminergic neuron Present 3H VTA Both Th+beta-gal+ and Th+beta-gal- neurons were present.
Specimen 4D lateral to medial 1: embryonic day 14.5; Nkx2-9tm1Jlck/Nkx2-9tm1Jlck (more )
Structure Level Pattern Image Note
TS22: midbrain ventricular layer Present 4D-1 Increased expression in the (sub)ventricular zone.
Specimen 4D lateral to medial 2: embryonic day 14.5; Nkx2-9tm1Jlck/Nkx2-9tm1Jlck (more )
Structure Level Pattern Image Note
TS22: midbrain ventricular layer Present 4D-2 Increased expression in the (sub)ventricular zone.
TS22: midbrain Weak Regionally restricted 4D-2 Decreased expression in mesodiencephalic dopaminergic neuronal region.
Specimen 4D lateral to medial 3: embryonic day 14.5; Nkx2-9tm1Jlck/Nkx2-9tm1Jlck (more )
Structure Level Pattern Image Note
TS22: midbrain ventricular layer Present 4D-3 Increased expression in the (sub)ventricular zone.
TS22: midbrain Weak Regionally restricted 4D-3 Decreased expression in mesodiencephalic dopaminergic neuronal region.
Specimen 4D lateral to medial 4: embryonic day 14.5; Nkx2-9tm1Jlck/Nkx2-9tm1Jlck (more )
Structure Level Pattern Image Note
TS22: midbrain ventricular layer Present 4D-4 Increased expression in the (sub)ventricular zone.
Specimen 4D lateral to medial 5: embryonic day 14.5; Nkx2-9tm1Jlck/Nkx2-9tm1Jlck (more )
Structure Level Pattern Image Note
TS22: midbrain ventricular layer Present 4D-5 Increased expression in the (sub)ventricular zone.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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01/06/2026
MGI 6.24 		The Jackson Laboratory