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Gene Expression Data
Assay Details
Assay
Reference: J:316482 Kouwenhoven WM, et al., Nkx2.9 Contributes to Mid-Hindbrain Patterning by Regulation of mdDA Neuronal Cell-Fate and Repression of a Hindbrain-Specific Cell-Fate. Int J Mol Sci. 2021 Nov 23;22(23)
Assay type: RNA in situ
MGI Accession ID: MGI:7576004
Gene symbol: Nkx2-9
Gene name: NK2 homeobox 9
Probe: Nkx2.9 cDNA
Probe preparation: Antisense labelled with digoxigenin RNA
Results
Specimen 1B WT lateral to medial 1: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: hindbrain Present Regionally restricted 1B WT-1 Expressed in the rostral hindbrain.
TS20: midbrain Present Regionally restricted 1B WT-1 Expressed in the dorsal midbrain.
TS20: zona limitans intrathalamica Present 1B WT-1
TS20: midbrain-hindbrain junction Strong Regionally restricted 1B WT-1 Expressed in the isthmic organizer.
Specimen 1B WT lateral to medial 2: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: hindbrain Present Regionally restricted 1B WT-2 Expressed in the rostral hindbrain.
TS20: midbrain Present Regionally restricted 1B WT-2 Expressed in the dorsal midbrain.
TS20: zona limitans intrathalamica Present 1B WT-2
TS20: midbrain-hindbrain junction Strong Regionally restricted 1B WT-2 Expressed in the isthmic organizer.
Specimen 1B WT lateral to medial 3: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: midbrain Present Regionally restricted 1B WT-3 Expressed in the dorsal midbrain.
TS20: hindbrain Present Regionally restricted 1B WT-3 Expressed in the rostral hindbrain.
TS20: zona limitans intrathalamica Present 1B WT-3
TS20: midbrain-hindbrain junction Strong Regionally restricted 1B WT-3 Expressed in the isthmic organizer.
Specimen 1B WT lateral to medial 4: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: hindbrain Present Regionally restricted 1B WT-4 Expressed in the rostral hindbrain.
TS20: midbrain Present Regionally restricted 1B WT-4 Expressed in the dorsal midbrain.
TS20: zona limitans intrathalamica Present 1B WT-4
TS20: midbrain-hindbrain junction Strong Regionally restricted 1B WT-4 Expressed in the isthmic organizer.
Specimen 1B WT lateral to medial 5: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: hindbrain Present Regionally restricted 1B WT-5 Expressed in the rostral hindbrain.
TS20: midbrain Present Regionally restricted 1B WT-5 Expressed in the dorsal midbrain.
TS20: zona limitans intrathalamica Present 1B WT-5
TS20: midbrain-hindbrain junction Present Regionally restricted 1B WT-5 Expressed in the isthmic organizer.
Specimen 1B En1KO lateral to medial 1: embryonic day 12.5; En1tm1Alj/En1tm1Alj (more )
Structure Level Pattern Image Note
TS20: zona limitans intrathalamica Present 1B En1KO-1
Specimen 1B En1KO lateral to medial 2: embryonic day 12.5; En1tm1Alj/En1tm1Alj (more )
Structure Level Pattern Image Note
TS20: zona limitans intrathalamica Present 1B En1KO-2
TS20: midbrain-hindbrain junction Absent 1B En1KO-2 No expression in the isthmic organizer.
Specimen 1B En1KO lateral to medial 3: embryonic day 12.5; En1tm1Alj/En1tm1Alj (more )
Structure Level Pattern Image Note
TS20: zona limitans intrathalamica Present 1B En1KO-3
TS20: midbrain-hindbrain junction Absent 1B En1KO-3 No expression in the isthmic organizer.
Specimen 1B En1KO lateral to medial 4: embryonic day 12.5; En1tm1Alj/En1tm1Alj (more )
Structure Level Pattern Image Note
TS20: zona limitans intrathalamica Present 1B En1KO-4
TS20: midbrain-hindbrain junction Absent 1B En1KO-4 No expression in the isthmic organizer.
Specimen 1B En1KO lateral to medial 5: embryonic day 12.5; En1tm1Alj/En1tm1Alj (more )
Structure Level Pattern Image Note
TS20: zona limitans intrathalamica Present 1B En1KO-5
TS20: midbrain-hindbrain junction Absent 1B En1KO-5 No expression in the isthmic organizer.
Specimen 2B lateral to medial 1: embryonic day 12.5 (more )
Note: Double labeled: blue - Nkx2-9; brown - Th (assay MGI:7576023).
Structure Level Pattern Image Note
TS20: metencephalon Present 2B-1
TS20: midbrain ventricular layer Present 2B-1
TS20: midbrain Present Regionally restricted 2B-1 Expressed in the ventricular and subventricular zones.
Specimen 2B lateral to medial 2: embryonic day 12.5 (more )
Note: Double labeled: blue - Nkx2-9; brown - Th (assay MGI:7576023).
Structure Level Pattern Image Note
TS20: diencephalon Present 2B-2
TS20: metencephalon Present 2B-2
TS20: midbrain ventricular layer Present 2B-2
TS20: midbrain Present Regionally restricted 2B-2 Expressed in the ventricular and subventricular zones.
Specimen 2B lateral to medial 3: embryonic day 12.5 (more )
Note: Double labeled: blue - Nkx2-9; brown - Th (assay MGI:7576023).
Structure Level Pattern Image Note
TS20: diencephalon Present 2B-3
TS20: metencephalon Present 2B-3
TS20: midbrain ventricular layer Present 2B-3
TS20: zona limitans intrathalamica Present 2B-3
TS20: midbrain Present Regionally restricted 2B-3 Expressed in the ventricular and subventricular zones.
Specimen 2B lateral to medial 4: embryonic day 12.5 (more )
Note: Double labeled: blue - Nkx2-9; brown - Th (assay MGI:7576023).
Structure Level Pattern Image Note
TS20: diencephalon Present 2B-4
TS20: metencephalon Present 2B-4
TS20: midbrain ventricular layer Present 2B-4
TS20: midbrain Present Regionally restricted 2B-4 Expressed in the ventricular and subventricular zones.
Specimen 2B lateral to medial 5: embryonic day 12.5 (more )
Note: Double labeled: blue - Nkx2-9; brown - Th (assay MGI:7576023).
Structure Level Pattern Image Note
TS20: metencephalon Present 2B-5
TS20: midbrain ventricular layer Present 2B-5
TS20: midbrain Present Regionally restricted 2B-5 Expressed in the ventricular and subventricular zones. Expressed in such proximity to the TH protein that possible colocalization cannot be excluded.
Specimen 2B lateral to medial 6: embryonic day 12.5 (more )
Note: Double labeled: blue - Nkx2-9; brown - Th (assay MGI:7576023).
Structure Level Pattern Image Note
TS20: midbrain Present Regionally restricted 2B-6 Expressed in the ventricular and subventricular zones. Expressed in such proximity to the TH protein that possible colocalization cannot be excluded.
TS20: midbrain ventricular layer Present 2B-6
TS20: midbrain-hindbrain junction Present 2B-6
Specimen 2B lateral to medial 7: embryonic day 12.5 (more )
Note: Double labeled: blue - Nkx2-9; brown - Th (assay MGI:7576023).
Structure Level Pattern Image Note
TS20: midbrain-hindbrain junction Present 2B-7 Expression was dorsal-caudal to the TH expression domain.
Specimen 2C: embryonic day 12.5 (more )
Note: Pseudo-overlay: green - Nkx2-9; red - Fgf8 (assay MGI:7576024).
Structure Level Pattern Image Note
TS20: midbrain Present Regionally restricted 2C merge , 2C Nkx2.9 Coexpressed with Fgf8 in the isthmic organizer.
Specimen 2D lateral to medial 1: embryonic day 14.5 (more )
Structure Level Pattern Image Note
TS22: midbrain Present Regionally restricted 2D-1 Expression was limited to a small area in the most caudal border of the midbrain, close to the isthmic organizer.
Specimen 2D lateral to medial 2: embryonic day 14.5 (more )
Structure Level Pattern Image Note
TS22: midbrain Present Regionally restricted 2D-2 Expression was limited to a small area in the most caudal border of the midbrain, close to the isthmic organizer.
Specimen 2D lateral to medial 3: embryonic day 14.5 (more )
Structure Level Pattern Image Note
TS22: midbrain Present Regionally restricted 2D-3 Expression was limited to a small area in the most caudal border of the midbrain, close to the isthmic organizer.
Specimen 2D lateral to medial 4: embryonic day 14.5 (more )
Structure Level Pattern Image Note
TS22: midbrain Present Regionally restricted 2D-4 Expression was limited to a small area in the most caudal border of the midbrain, close to the isthmic organizer.
Specimen 2D lateral to medial 5: embryonic day 14.5 (more )
Structure Level Pattern Image Note
TS22: midbrain Present Regionally restricted 2D-5 Expression was limited to a small area in the most caudal border of the midbrain, close to the isthmic organizer.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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