Probe:	Lin28a cDNA
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS17: neural tube	Strong	Regionally restricted	5Ba inset , 5Ba	Expressed in the caudal part.
TS17: tail mesenchyme	Strong	Regionally restricted	5Ba , 5Ba inset	Expressed in the ventral mesoderm adjacent the neural tube.
TS17: tail neural tube	Strong		5Ba , 5Ba inset

Structure	Level	Pattern	Image	Note
TS17: tail mesenchyme	Strong		5Be , 5Be inset	Stronger expression compared with the wild-type.
TS17: tail neural tube	Strong		5Be , 5Be inset	Stronger expression compared with the wild-type.
TS17: neural tube	Strong		5Be	Grossly resembled the pattern observed in wild-type embryos, although the signal in the tail bud appeared stronger.
TS17: tail	Strong		5Be	Grossly resembled the pattern observed in wild-type embryos, although the signal in the tail bud appeared stronger.

Structure	Level	Pattern	Image	Note
TS19: tail mesenchyme	Weak		5Bi , 5Bi inset	Strongly down-regulated expression in the entire tail region.
TS19: tail neural tube	Weak		5Bi , 5Bi inset	Strongly down-regulated expression in the entire tail region.

Structure	Level	Pattern	Image	Note
TS19: tail mesenchyme	Strong		5Bm inset , 5Bm	Remained robustly expressed.
TS19: tail neural tube	Strong		5Bm inset , 5Bm	Remained robustly expressed.

Structure	Level	Pattern	Image	Note
TS17: neural tube	Strong	Regionally restricted	6J , 6J inset	Expressed in the caudal part.
TS17: tail mesenchyme	Strong	Regionally restricted	6J inset , 6J	Expressed in the ventral mesoderm adjacent the neural tube.
TS17: tail neural tube	Strong		6J inset , 6J

Structure	Level	Pattern	Image	Note
TS17: neural tube	Strong	Regionally restricted	6M , 6M inset	Expressed in the caudal part.
TS17: tail mesenchyme	Strong	Regionally restricted	6M inset , 6M	Expressed in the ventral mesoderm adjacent the neural tube.
TS17: tail neural tube	Strong		6M inset , 6M

Structure	Level	Pattern	Image	Note
TS17: tail mesenchyme	Strong		6N inset , 6N	A slight but consistent increase in expression, compared with the wild-type.
TS17: tail neural tube	Strong		6N inset , 6N	A slight but consistent increase in expression, compared with the wild-type.

Structure	Level	Pattern	Image	Note
TS17: tail neural tube	Strong	Regionally restricted	S3Ea
TS17: tail mesenchyme	Strong	Regionally restricted	S3Ea	Expressed in the ventral mesoderm adjacent the neural tube.

Structure	Level	Pattern	Image	Note
TS17: tail neural tube	Strong		S3Eb	Stronger expression compared with the wild-type. Ectopic epithelial pocket was seen.
TS17: tail mesenchyme	Strong		S3Eb	Stronger expression compared with the wild-type. Ectopic epithelial pocket was seen.

Structure	Level	Pattern	Image	Note
TS19: tail mesenchyme	Weak		S3Ec	Strongly down-regulated expression in the entire tail region. In this section an absence of any detectable transcripts was shown.
TS19: tail neural tube	Weak		S3Ec	Strongly down-regulated expression in the entire tail region. In this section an absence of any detectable transcripts was shown.

Structure	Level	Pattern	Image	Note
TS19: tail mesenchyme	Strong		S3Ed	Remained robustly expressed.
TS19: tail neural tube	Strong		S3Ed	Remained robustly expressed.