Probe:	Robo1 cDNA
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS20: retina	Absent		2B

Structure	Cell Type	Level	Pattern	Image	Note
TS22: retina	retinal ganglion cell	Present	Regionally restricted	2P , 2G	Expressed throughout retinal ganglion cell layer, although restricted to a scattered subpopulation of cells.

Structure	Level	Pattern	Image	Note
TS25: retina layer	Present	Regionally restricted	2L	Expressed at the inner nuclear layer.

Structure	Level	Pattern	Image	Note
TS20: diencephalon	Present	Regionally restricted	3E	Expressed in a domain posterior and medial to the CD44/SSEA (Fut4) neurons.

Structure	Level	Pattern	Image	Note
TS20: diencephalon	Present	Regionally restricted	3F	Expressed in a domain posterior and medial to the CD44/SSEA (Fut4) neurons.

Structure	Level	Pattern	Image	Note
TS20: diencephalon	Present	Regionally restricted	3G	Expressed in a domain posterior and medial to the CD44/SSEA (Fut4) neurons.

Structure	Level	Pattern	Image	Note
TS20: diencephalon	Present	Regionally restricted	3H	Expressed in a domain posterior and medial to the CD44/SSEA (Fut4) neurons.

Structure	Level	Pattern	Image	Note
TS22: diencephalon	Present	Regionally restricted	4E	More weakly expressed throughout the diencephalon, both posterior to the CD44/SSEA (Fut4) neurons and around the third ventricle.

Structure	Level	Pattern	Image	Note
TS22: diencephalon	Present	Regionally restricted	4F	More weakly expressed throughout the diencephalon, both posterior to the CD44/SSEA (Fut4) neurons and around the third ventricle.

Structure	Level	Pattern	Image	Note
TS22: diencephalon	Present	Regionally restricted	4G	More weakly expressed throughout the diencephalon, both posterior to the CD44/SSEA (Fut4) neurons and around the third ventricle.

Structure	Level	Pattern	Image	Note
TS22: diencephalon	Present	Regionally restricted	4H	More weakly expressed throughout the diencephalon, both posterior to the CD44/SSEA (Fut4) neurons and around the third ventricle.

Structure	Level	Pattern	Image	Note
TS25: diencephalon	Present	Regionally restricted	5B	More weakly expressed throughout the diencephalon, both posterior to the CD44/SSEA (Fut4) neurons and around the third ventricle.

Structure	Cell Type	Level	Pattern	Image	Note
TS23: retina layer	retinal ganglion cell	Present	Regionally restricted		Expressed throughout retinal ganglion cell layer.
TS23: diencephalon		Present	Regionally restricted		More weakly expressed throughout the diencephalon, both posterior to the CD44/SSEA (Fut4) neurons and around the third ventricle.