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Gene Expression Data
Assay Details
Assay
Reference: J:265272 Ghanem LR, et al., Poly(C)-Binding Protein Pcbp2 Enables Differentiation of Definitive Erythropoiesis by Directing Functional Splicing of the Runx1 Transcript. Mol Cell Biol. 2018 Aug 15;38(16)
Assay type: RT-PCR
MGI Accession ID: MGI:7564238
Gene symbol: Hbb-bh1
Gene name: hemoglobin Z, beta-like embryonic chain
Probe: MHB1672, MHB1673
Probe preparation: labelled with SYBR green
Visualized with: Phosphorimaging
Assay notes: Quantitative RT-PCR. Normalized expression of mRNAs from WT mice was defined as 1.0 in each case.
Results Image: 7D Hbb-bh1
 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified Amount Genetic BackgroundMutant Allele(s)Sex
DNA Ladder Mol. Wt. Marker Lane
Runx+/+ E12.5 TS20: liver Present 0.4 µg; total RNA involves: C57BL/6J * SJL/J Not Specified
Runx+/deltaE6 E12.5 TS20: liver Present 0.4 µg; total RNA involves: C57BL/6J * SJL/J Runx1em1Salr/Runx1+Not Specified
RunxdeltaE6/deltaE6 E12.5 TS20: liver Present (a) 0.4 µg; total RNA involves: C57BL/6J * SJL/J Runx1em1Salr/Runx1em1SalrNot Specified
- Other lane(s) in blot assay different gene(s)
Notes:
(a) There is a significant increase in expression in this mutant compared to wild-type.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/14/2024
MGI 6.23 		The Jackson Laboratory