Runx1 Western blot Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:265272 Ghanem LR, et al., Poly(C)-Binding Protein Pcbp2 Enables Differentiation of Definitive Erythropoiesis by Directing Functional Splicing of the Runx1 Transcript. Mol Cell Biol. 2018 Aug 15;38(16)
Assay type:	Western blot
MGI Accession ID:	MGI:7564232
Gene symbol:	Runx1
Gene name:	runt related transcription factor 1

Antibody:	anti-Runx1 (ab92336)
Detection system:	Secondary antibody

Results

Image: 5E

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	46 kDa	Genetic Background	Mutant Allele(s)	Sex
Ladder	Mol. Wt. Marker Lane
Runx^+/+	E14.5	TS22: liver	Present (a)	involves: C57BL/6J * SJL/J		Not Specified
Runx^+/deltaE6	E14.5	TS22: liver	Present (b)	involves: C57BL/6J * SJL/J	Runx1^em1Salr/Runx1⁺	Not Specified
Runx^{deltaE6/deltaE6}	E14.5	TS22: liver	Present (c)	involves: C57BL/6J * SJL/J	Runx1^em1Salr/Runx1^em1Salr	Not Specified

Notes:
(a) There are 2 bands and this shorter band (45-47kDa) is the isoform with the excluded exon 6 and it represents 40% of the total protein.
(b) There are 2 bands and this shorter band (45-47kDa) is the isoform with the excluded exon 6 and it represents 77% of the total protein.
(c) This single band is the isoform with the excluded exon 6.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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