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Gene Expression Data
Assay Details
Assay
Reference: J:217461 Yang G, et al., An eIF4E1/4E-T complex determines the genesis of neurons from precursors by translationally repressing a proneurogenic transcription program. Neuron. 2014 Nov 19;84(4):723-39
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7564070
Gene symbol: Eif4enif1
Gene name: eukaryotic translation initiation factor 4E nuclear import factor 1
Antibody: anti-EIF4ENIF1
Detection system: Secondary antibody coupled to Alexa Fluor
Assay notes: Two Eif4enif1 antibodies were described, but the one used in this assay was not detailed. Eif4enif1 antibody MGI:7564034 may have been used in this assay.
Results
Specimen 4B 4E-T: embryonic day 14.5 (more )
Note: Double labeled: green - Eif4enif1; red - Eif4e (assay MGI:7564065).
Structure Level Pattern Image Note
TS22: cerebral cortex subventricular zone Present Regionally restricted 4B 4E-T , 4B (merged) Authors report expession in same cells as Eif4e.
TS22: cerebral cortex ventricular layer Present Regionally restricted 4B 4E-T , 4B (merged) Authors report expession in same cells as Eif4e.
Specimen 4B 4E-T (left): embryonic day 14.5 (more )
Note: Double labeled: green - Eif4enif1; red - Eif4e (assay MGI:7564065).
Structure Level Pattern Image Note
TS22: cerebral cortex ventricular layer Present Regionally restricted 4D (merged left) , 4D 4E-T (left) Expression colocalized with Eif4e in subpopulation of punctate cytoplasmic foci in radial precursor cell bodies.
Specimen 4B 4E-T (right): embryonic day 14.5 (more )
Note: Double labeled: green - Eif4enif1; red - Eif4e (assay MGI:7564065).
Structure Level Pattern Image Note
TS22: cerebral cortex ventricular layer Present Regionally restricted 4D (merged right) , 4D 4E-T (right) Expression colocalized with Eif4e in apical endfeet adjacent to ventricle.
Specimen 7B 4E-T: embryonic day 15.0 (more )
Note: Double labeled: green - Eif4enif1; red - Neurog1 (assay MGI:7564090).
Structure Level Pattern Image Note
TS23: cerebral cortex ventricular layer Present Regionally restricted 7B Merge inset , 7B 4E-T , 7B 4E-T XZ , 7B Merge YZ , 7B 4E-T YZ , 7B 4E-T inset , 7B Merge , 7B Merge XZ Expression partially colocalized with Neurog1 expressing foci.
Specimen 7C 4E-T: embryonic day 15.0 (more )
Note: Double labeled: green - Eif4enif1; red - Neurog2 (assay MGI:7564091).
Structure Level Pattern Image Note
TS23: cerebral cortex ventricular layer Present Regionally restricted 7C 4E-T YZ , 7C 4E-T XZ , 7C Merge XZ , 7C 4E-T , 7C Merge , 7C Merge YZ Expression partially colocalized with Neurog2 expressing foci.
Specimen 7D 4E-T: embryonic day 15.0 (more )
Note: Double labeled: green - Eif4enif1; red - Neurod1 (assay MGI:7564093).
Structure Level Pattern Image Note
TS23: cerebral cortex ventricular layer Present Regionally restricted 7D Merge , 7D Merge XZ , 7D Merge YZ , 7D 4E-T , 7D 4E-T YZ , 7D 4E-T XZ Expression partially colocalized with Neurod1 expressing foci.
Specimen 7E 4E-T: embryonic day 15.0 (more )
Note: Double labeled: green - Eif4enif1; red - Neurog1 (assay MGI:7564090).
Structure Level Pattern Image Note
TS22: cerebral cortex subventricular zone Present Regionally restricted 7E (left) Expression partially colocalized with Neurog1 expression.
TS22: cerebral cortex ventricular layer Present Regionally restricted 7E (left) Expression partially colocalized with Neurog1 expression.
Specimen 7E 4E-T (top): embryonic day 15.0 (more )
Note: Double labeled: green - Eif4enif1; red - Neurog1 (assay MGI:7564090).
Structure Level Pattern Image Note
TS22: cerebral cortex subventricular zone Present Regionally restricted 7E (top right)
Specimen 7E 4E-T (middle): embryonic day 15.0 (more )
Note: Double labeled: green - Eif4enif1; red - Neurog1 (assay MGI:7564090).
Structure Level Pattern Image Note
TS23: cerebral cortex subventricular zone Present Regionally restricted 7E (right middle)
TS23: cerebral cortex ventricular layer Present Regionally restricted 7E (right middle)
Specimen 7E 4E-T (bottom): embryonic day 15.0 (more )
Note: Double labeled: green - Eif4enif1; red - Neurog1 (assay MGI:7564090).
Structure Level Pattern Image Note
TS23: cerebral cortex ventricular layer Present Regionally restricted 7E (right middle) Highest proportion of Neurog1-positive, Eif4enif1-positive foci in apical-most part of ventricular zone (bin 1).
Specimen S5C 4E-T (upper): embryonic day 15.0 (more )
Note: Double labeled: green - Eif4enif1; red - Neurog1 (assay MGI:7564090).
Structure Level Pattern Image Note
TS23: cerebral cortex ventricular layer Present Regionally restricted S5C 4E-T (upper) , S5C Merge (upper)
Specimen S5C 4E-T (lower): embryonic day 15.0 (more )
Note: Double labeled: green - Eif4enif1; red - Neurog1 (assay MGI:7564090).
Structure Level Pattern Image Note
TS23: cerebral cortex ventricular layer Present Regionally restricted S5C 4E-T (lower) , S5C Merge (lower)
Specimen S5E 4E-T (top): embryonic day 12.0 (more )
Note: Double labeled: green - Eif4enif1; red - Neurog1 (assay MGI:7564090).
Structure Level Pattern Image Note
TS20: cerebral cortex Present
Specimen S5E 4E-T (middle): embryonic day 12.0 (more )
Note: Double labeled: green - Eif4enif1; red - Glo1 (assay MGI:7564096).
Structure Level Pattern Image Note
TS20: cerebral cortex Present S5E (middle)
Specimen S5E 4E-T (bottom): embryonic day 12.0 (more )
Note: Double labeled: green - Eif4enif1; red - Glo1 (assay MGI:7564096).
Structure Level Pattern Image Note
TS20: cerebral cortex Present S5E (bottom)
Specimen 7I 4E-T: embryonic day 15.0 (more )
Note: Triple labeled: green - Eif4enif1; red - Neurog1 (assay MGI:7564090); blue - Neurog1 (assay MGI:7564071).
Structure Level Pattern Image Note
TS23: cerebral cortex Present Regionally restricted 7I (left) Neurog1 protein in complexes containing Eif4nif1 protein and Neurog1 RNA.
Specimen 7I 4E-T (upper): embryonic day 15.0 (more )
Note: Triple labeled: green - Eif4enif1; red - Neurog1 (assay MGI:7564090); blue - Neurog1 (assay MGI:7564071).
Structure Level Pattern Image Note
TS23: cerebral cortex Present Regionally restricted 7I Merge (upper) , 7I 4E-T (upper) Neurog1 protein in complexes containing Eif4nif1 protein and Neurog1 RNA.
Specimen 7I 4E-T (lower): embryonic day 15.0 (more )
Note: Triple labeled: green - Eif4enif1; red - Neurog1 (assay MGI:7564090); blue - Neurog1 (assay MGI:7564071).
Structure Level Pattern Image Note
TS23: cerebral cortex Present Regionally restricted 7I Merge (lower) , 7I 4E-T (lower) Neurog1 protein in complexes containing Eif4nif1 protein and Neurog1 RNA.
Specimen 8A 4E-T: embryonic day 12.0 (more )
Note: Double labeled: green - Eif4enif1; red - Neurog2 (assay MGI:7564091).
Structure Level Pattern Image Note
TS20: cerebral cortex subventricular zone Present 8A (left)
TS20: cerebral cortex ventricular layer Present 8A (left)
Specimen 8A 4E-T (top): embryonic day 12.0 (more )
Note: Double labeled: green - Eif4enif1; red - Neurog2 (assay MGI:7564091).
Structure Level Pattern Image Note
TS20: cerebral cortex subventricular zone Present 8A (top right)
Specimen 8A 4E-T (middle): embryonic day 12.0 (more )
Note: Double labeled: green - Eif4enif1; red - Neurog2 (assay MGI:7564091).
Structure Level Pattern Image Note
TS20: cerebral cortex subventricular zone Present 8A (middle)
TS20: cerebral cortex ventricular layer Present 8A (middle)
Specimen 8A 4E-T (bottom): embryonic day 12.0 (more )
Note: Double labeled: green - Eif4enif1; red - Neurog2 (assay MGI:7564091).
Structure Level Pattern Image Note
TS20: cerebral cortex ventricular layer Present 8A (bottom)
Specimen 8E 4E-T: embryonic day 12.0 (more )
Note: Triple labeled: green - Eif4enif1; red - Neurog2 (assay MGI:7564091); blue - Neurog2 (assay MGI:7564072).
Structure Level Pattern Image Note
TS20: cerebral cortex subventricular zone Present 8E (left)
TS20: cerebral cortex ventricular layer Present 8E (left)
Specimen 8E 4E-T (upper): embryonic day 12.0 (more )
Note: Triple labeled: green - Eif4enif1; red - Neurog2 (assay MGI:7564091); blue - Neurog2 (assay MGI:7564072).
Structure Level Pattern Image Note
TS20: cerebral cortex subventricular zone Present 8E 4E-T (upper) , 8E Merge (upper)
TS20: cerebral cortex ventricular layer Present 8E 4E-T (upper) , 8E Merge (upper)
Specimen 8E 4E-T (lower): embryonic day 12.0 (more )
Note: Triple labeled: green - Eif4enif1; red - Neurog2 (assay MGI:7564091); blue - Neurog2 (assay MGI:7564072).
Structure Level Pattern Image Note
TS20: cerebral cortex subventricular zone Present 8E 4E-T (lower) , 8E Merge (lower)
TS20: cerebral cortex ventricular layer Present 8E 4E-T (lower) , 8E Merge (lower)
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/30/2024
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