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Gene Expression Data
Assay Details
Assay
Reference: J:217461 Yang G, et al., An eIF4E1/4E-T complex determines the genesis of neurons from precursors by translationally repressing a proneurogenic transcription program. Neuron. 2014 Nov 19;84(4):723-39
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7564065
Gene symbol: Eif4e
Gene name: eukaryotic translation initiation factor 4E
Antibody: anti-eIF4E1
Detection system: Secondary antibody coupled to Alexa Fluor
Assay notes: Two Eif4e antibodies were described, but the one used in this assay was not detailed. Eif4e antibody name MGI:7564032 may have been used in this assay.
Results
Specimen 1C eIF4E1: embryonic day 14.5 (more )
Structure Level Pattern Image Note
TS22: cerebral cortex subventricular zone Strong Regionally restricted 1C eIF4E1 Robust expression in ventricular and subventricular zones.
TS22: cerebral cortex ventricular layer Strong Regionally restricted 1C eIF4E1 Robust expression in ventricular and subventricular zones.
TS22: cortical plate Present 1C eIF4E1
TS22: cerebral cortex intermediate zone Weak 1C eIF4E1
Specimen 1C (Pax6): embryonic day 14.5 (more )
Note: Double labeled: red - Eif4e; green - Pax6 (assay MGI:7564066).
Structure Level Pattern Image Note
TS22: cerebral cortex subventricular zone Strong Regionally restricted 1C Pax6
TS22: cerebral cortex ventricular layer Strong Regionally restricted 1C Pax6
TS22: cortical plate Present 1C Pax6
TS22: cerebral cortex intermediate zone Weak 1C Pax6
Specimen 1C (Unc5d): embryonic day 14.5 (more )
Note: Double labeled: red - Eif4e; green - Unc5d (assay MGI:7564067).
Structure Level Pattern Image Note
TS22: cerebral cortex subventricular zone Strong Regionally restricted 1C UNC5D Expression localized to punctate cytoplasmic granules in radial precursors expressing Pax6.
TS22: cerebral cortex ventricular layer Strong Regionally restricted 1C UNC5D Expression localized to punctate cytoplasmic granules in radial precursors expressing Pax6.
TS22: cortical plate Present 1C UNC5D
TS22: cerebral cortex intermediate zone Weak 1C UNC5D
Specimen 1D: embryonic day 14.5 (more )
Structure Level Pattern Image Note
TS22: cerebral cortex ventricular layer Present Regionally restricted 1D Expression localized to punctate cytoplasmic granules.
Specimen 4B eIF4E1: embryonic day 14.5 (more )
Note: Double labeled: red - Eif4e; green - Eif4enif1 (assay MGI:7564070).
Structure Level Pattern Image Note
TS22: cerebral cortex subventricular zone Present Regionally restricted 4B eIF4E1 , 4B (merged) Authors report expession in same cells as Eif4enif1.
TS22: cerebral cortex ventricular layer Present Regionally restricted 4B eIF4E1 , 4B (merged) Authors report expession in same cells as Eif4enif1.
Specimen 4B eIF4E1 (left): embryonic day 14.5 (more )
Note: Double labeled: red - Eif4e; green - Eif4enif1 (assay MGI:7564070).
Structure Level Pattern Image Note
TS22: cerebral cortex ventricular layer Present Regionally restricted 4D eiF4E1 (left) , 4D (merged left) Expression colocalized with Eif4enif1 in subpopulation of punctate cytoplasmic foci in radial precursor cell bodies.
Specimen 4B eIF4E1 (right): embryonic day 14.5 (more )
Note: Double labeled: red - Eif4e; green - Eif4enif1 (assay MGI:7564070).
Structure Level Pattern Image Note
TS22: cerebral cortex ventricular layer Present Regionally restricted 4D eiF4E1 (right) , 4D (merged right) Expression colocalized with Eif4enif1 in apical endfeet adjacent to ventricle.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/30/2024
MGI 6.23 		The Jackson Laboratory