Gene Expression Data
Assay Details
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Assay
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Reference:
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J:186504
Tanimoto Y, et al., Prevention of premature fusion of calvarial suture in GLI-Kruppel family member 3 (Gli3)-deficient mice by removing one allele of Runt-related transcription factor 2 (Runx2). J Biol Chem. 2012 Jun 15;287(25):21429-38
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7544958
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Gene symbol:
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Smad9
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Gene name:
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SMAD family member 9
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Antibody:
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anti-phospho-Smad1/5/9 (9511)
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin
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Assay notes:
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The antibody used recognizes the phosphorylated form of the protein. The specificity of the antibody used was not detailed; all family members have been annotated.
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Results
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Specimen
7Da:
embryonic day 15.5
(more  )
Note: lambdoid suture
Specimen
7Da:
(close  )
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Genetic Background:
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involves: C3H/HeJ * C57BL/6
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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lambdoid suture
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Specimen
7Db:
embryonic day 15.5; Gli3Xt-J/ Gli3Xt-J
(more )
Note: lambdoid suture
Specimen
7Db:
(close )
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Genetic Background:
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involves: C3H/HeJ * C57BL/6
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Mutant Allele(s):
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Gli3Xt-J/Gli3Xt-J
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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lambdoid suture
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: chondrocranium |
Strong |
Regionally restricted |
7Db
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Ectopic expression in the mid-sutural mesenchymal cells throughout the suture area.
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Specimen
7Dc:
(close )
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Genetic Background:
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involves: C3H/HeJ * C57BL/6
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Mutant Allele(s):
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Gli3Xt-J/Gli3Xt-J
Runx2tm1Mjo/Runx2+
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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lambdoid suture
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Specimen
7Dd:
embryonic day 15.5
(more )
Note: coronal suture
Specimen
7Dd:
(close )
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Genetic Background:
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involves: C3H/HeJ * C57BL/6
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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coronal suture
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Specimen
7De:
embryonic day 15.5; Gli3Xt-J/ Gli3Xt-J
(more )
Note: coronal suture
Specimen
7De:
(close )
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Genetic Background:
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involves: C3H/HeJ * C57BL/6
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Mutant Allele(s):
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Gli3Xt-J/Gli3Xt-J
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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coronal suture
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: frontal bone primordium |
Present |
Regionally restricted |
7De
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Expressed in osteogenic fronts in the nuclei, similar as in the wild-type.
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| TS23: parietal bone |
Present |
Regionally restricted |
7De
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Expressed in osteogenic fronts in the nuclei, similar as in the wild-type.
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| TS23: periosteum |
Present |
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7De
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Expressed in the nuclei, similar as in the wild-type.
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Specimen
7Df:
(close )
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Genetic Background:
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involves: C3H/HeJ * C57BL/6
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Mutant Allele(s):
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Gli3Xt-J/Gli3Xt-J
Runx2tm1Mjo/Runx2+
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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coronal suture
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: frontal bone primordium |
Present |
Regionally restricted |
7Df
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Expressed in osteogenic fronts in the nuclei, similar as in the wild-type.
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| TS23: parietal bone |
Present |
Regionally restricted |
7Df
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Expressed in osteogenic fronts in the nuclei, similar as in the wild-type.
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| TS23: periosteum |
Present |
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7Df
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Expressed in the nuclei, similar as in the wild-type.
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Specimen
7Dg:
embryonic day 15.5
(more )
Note: interfrontal suture
Specimen
7Dg:
(close )
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Genetic Background:
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involves: C3H/HeJ * C57BL/6
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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interfrontal suture
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Specimen
7Dh:
embryonic day 15.5; Gli3Xt-J/ Gli3Xt-J
(more )
Note: interfrontal suture
Specimen
7Dh:
(close )
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Genetic Background:
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involves: C3H/HeJ * C57BL/6
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Mutant Allele(s):
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Gli3Xt-J/Gli3Xt-J
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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interfrontal suture
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: chondrocranium |
Strong |
Regionally restricted |
7Dh
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Ectopic expression in the mid-sutural mesenchymal cells throughout the suture area.
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Specimen
7Di:
(close )
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Genetic Background:
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involves: C3H/HeJ * C57BL/6
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Mutant Allele(s):
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Gli3Xt-J/Gli3Xt-J
Runx2tm1Mjo/Runx2+
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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interfrontal suture
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: periosteum |
Present |
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7Di
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Expressed in the nuclei, similar as in the wild-type.
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| TS23: frontal bone primordium |
Present |
Regionally restricted |
7Di
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Expressed in osteogenic fronts in the nuclei, similar as in the wild-type.
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