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Gene Expression Data
Assay Details
Assay
Reference: J:338243 Christova T, et al., LTK and ALK promote neuronal polarity and cortical migration by inhibiting IGF1R activity. EMBO Rep. 2023 Jul 5;24(7):e56937
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7544892
Gene symbol: Bcl11b
Gene name: B cell leukemia/lymphoma 11B
Antibody: anti-CTIP2 [25B6] (ab18465)
Detection system: Secondary antibody coupled to Alexa Fluor
Results
Specimen 1B WT: postnatal day 2 (more )
Note: Double labeled: red - Bcl11b; green - Satb2 (assay MGI:7544893).
Structure Cell Type Level Pattern Image Note
TS27: cerebral cortex intermediate zone neuron Present Regionally restricted 1B WT Satb2/Ctip2 At P2, immunolabeled cells were primarily localized to the intermediate zone (bins 5/6).

Specimen 1B DKO: postnatal day 2; Alktm1Swm/Alktm1Swm, Ltktm1Rabr/Ltktm1Rabr (more )
Note: Double labeled: red - Bcl11b; green - Satb2 (assay MGI:7544893).
Structure Cell Type Level Pattern Image Note
TS27: cerebral cortex subventricular zone neuron Present Regionally restricted 1B DKO Satb2/Ctip2 In the double knockout, immunolabeled cells were primarily localized to the subventricular zone/ventricular zone (bins 1-4).
TS27: cerebral cortex ventricular zone neuron Present Regionally restricted 1B DKO Satb2/Ctip2 In the double knockout, immunolabeled cells were primarily localized to the subventricular zone/ventricular zone (bins 1-4).

Specimen 1D WT: postnatal day 7 (more )
Note: Double labeled: red - Bcl11b; green - Satb2 (assay MGI:7544893).
Structure Cell Type Level Pattern Image Note
TS28: cortical layer V neuron Present 1D WT Satb2/Ctip2

Specimen 1D DKO: postnatal day 7; Alktm1Swm/Alktm1Swm, Ltktm1Rabr/Ltktm1Rabr (more )
Note: Double labeled: red - Bcl11b; green - Satb2 (assay MGI:7544893).
Structure Cell Type Level Pattern Image Note
TS28: cortical layer V neuron Present 1D DKO Satb2/Ctip2 The localization of the immunopositive neurons of the double knockout was more similar to wild type at P7.
TS28: cortical layer VI neuron Present 1D DKO Satb2/Ctip2 Some immunopositive cells were still observed in the lowest layers (bins 1-4, Layer VI) in the double knockout.

Specimen EV2C WT: postnatal day 2 (more )
Note: Double labeled: red - Bcl11b; green - Satb2 (assay MGI:7544893).
Structure Cell Type Level Pattern Image Note
TS27: cerebral cortex intermediate zone neuron Present Regionally restricted EV2C WT Satb2/Ctip2 At P2, immunolabeled cells were primarily localized to the intermediate zone (bins 5/6).

Specimen EV2C Alk-/-: postnatal day 2; Alktm1Swm/Alktm1Swm (more )
Note: Double labeled: red - Bcl11b; green - Satb2 (assay MGI:7544893).
Structure Cell Type Level Pattern Image Note
TS27: cerebral cortex neuron Present Regionally restricted EV2C Alk-/- Satb2/Ctip2 Modest disruptions in the immunolocalization were noted in the single mutant. Overall number of immunopositive neurons was similar to wild type.

Specimen EV2C Ltk-/-: postnatal day 2; Ltktm1Rabr/Ltktm1Rabr (more )
Note: Double labeled: red - Bcl11b; green - Satb2 (assay MGI:7544893).
Structure Cell Type Level Pattern Image Note
TS27: cerebral cortex neuron Present Regionally restricted EV2C Ltk-/- Satb2/Ctip2 Modest disruptions in the immunolocalization were noted in the single mutant. Overall number of immunopositive neurons was similar to wild type.

Specimen EV2E WT: postnatal day 7 (more )
Note: Double labeled: red - Bcl11b; green - Satb2 (assay MGI:7544893).
Structure Cell Type Level Pattern Image Note
TS28: cortical layer V neuron Present EV2E WT Satb2/Ctip2

Specimen EV2E Alk-/-: postnatal day 7; Alktm1Swm/Alktm1Swm (more )
Note: Double labeled: red - Bcl11b; green - Satb2 (assay MGI:7544893).
Structure Cell Type Level Pattern Image Note
TS28: cerebral cortex neuron Present Regionally restricted EV2E Alk-/- Satb2/Ctip2 Modest disruptions in the immunolocalization were noted in the single mutant. Overall number of immunopositive neurons was similar to wild type.

Specimen EV2E Ltk-/-: postnatal day 7; Ltktm1Rabr/Ltktm1Rabr (more )
Note: Double labeled: red - Bcl11b; green - Satb2 (assay MGI:7544893).
Structure Cell Type Level Pattern Image Note
TS28: cerebral cortex neuron Present Regionally restricted EV2E Ltk-/- Satb2/Ctip2 Modest disruptions in the immunolocalization were noted in the single mutant. Overall number of immunopositive neurons was similar to wild type.


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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
03/18/2025
MGI 6.24
The Jackson Laboratory