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Gene Expression Data
Assay Details
Assay
Reference: J:341081 Zhao C, et al., Differential CRH expression level determines efficiency of Cre- and Flp-dependent recombination. Front Neurosci. 2023;17:1163462
Assay type: In situ reporter (knock in)
MGI Accession ID: MGI:7541360
Gene symbol: Crh
Gene name: corticotropin releasing hormone
Reporter: Venus
Antibody: anti-GFP (abcam)
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen 4C - PVN: postnatal adult; Crhtm1(cre)Zjh/Crhtm1.1Ksak, Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. Double labeled: green - Crh; red - recombinase reporter.
Structure Level Pattern Image Note
TS28: paraventricular hypothalamic nucleus Present S1_Venus PVN , 4C Merge PVN , 4C Venus PVN , S1_Merge PVN

Specimen 4C - ME: postnatal adult; Crhtm1(cre)Zjh/Crhtm1.1Ksak, Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. Double labeled: green - Crh; red - recombinase reporter.
Structure Level Pattern Image Note
TS28: median eminence Present 4C Merge ME , 4C Venus ME

Specimen 4C - IPACL: postnatal adult; Crhtm1(cre)Zjh/Crhtm1.1Ksak, Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. Double labeled: green - Crh; red - recombinase reporter.
Structure Level Pattern Image Note
TS28: anterior commissure Present Regionally restricted 4C Merge IPACL , 4C Venus IPACL Expression is seen in the lateral part of the interstitial nucleus of the anterior commissure.

Specimen 4C - BNST: postnatal adult; Crhtm1(cre)Zjh/Crhtm1.1Ksak, Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. Double labeled: green - Crh; red - recombinase reporter.
Structure Level Pattern Image Note
TS28: bed nucleus of stria terminalis Present 4C Venus BNST , S1_Venus BNST , S1_Merge BNST , 4C Merge BNST

Specimen 4C - CeA: postnatal adult; Crhtm1(cre)Zjh/Crhtm1.1Ksak, Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. Double labeled: green - Crh; red - recombinase reporter.
Structure Level Pattern Image Note
TS28: central amygdaloid nucleus Present S1_Venus CeA , 4C Merge CeA , 4C Venus CeA , S1_Merge CeA

Specimen 4C - CTX: postnatal adult; Crhtm1(cre)Zjh/Crhtm1.1Ksak, Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. Double labeled: green - Crh; red - recombinase reporter.
Structure Level Pattern Image Note
TS28: cerebral cortex Present S1_Venus CTX , 4C Merge CTX , S1_Merge CTX , 4C Venus CTX

Specimen 4D - PVN: postnatal adult; Crhtm1.1(flpo)Bsab/Crhtm1.1Ksak, Gt(ROSA)26Sortm65.2(CAG-tdTomato)Hze/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. Double labeled: green - Crh; red - recombinase reporter.
Structure Level Pattern Image Note
TS28: paraventricular hypothalamic nucleus Present 4D Venus PVN , 4D Merge PVN

Specimen 4D - ME: postnatal adult; Crhtm1.1(flpo)Bsab/Crhtm1.1Ksak, Gt(ROSA)26Sortm65.2(CAG-tdTomato)Hze/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. Double labeled: green - Crh; red - recombinase reporter.
Structure Level Pattern Image Note
TS28: median eminence Present 4D Merge ME , 4D Venus ME

Specimen 4D - IPACL: postnatal adult; Crhtm1.1(flpo)Bsab/Crhtm1.1Ksak, Gt(ROSA)26Sortm65.2(CAG-tdTomato)Hze/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. Double labeled: green - Crh; red - recombinase reporter.
Structure Level Pattern Image Note
TS28: anterior commissure Present Regionally restricted 4D Merge IPACL , 4D Venus IPACL Expression is seen in the lateral part of the interstitial nucleus of the anterior commissure.

Specimen 4D - BNST: postnatal adult; Crhtm1.1(flpo)Bsab/Crhtm1.1Ksak, Gt(ROSA)26Sortm65.2(CAG-tdTomato)Hze/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. Double labeled: green - Crh; red - recombinase reporter.
Structure Level Pattern Image Note
TS28: bed nucleus of stria terminalis Present 4D Venus BNST , 4D Merge BNST

Specimen 4D - CeA: postnatal adult; Crhtm1.1(flpo)Bsab/Crhtm1.1Ksak, Gt(ROSA)26Sortm65.2(CAG-tdTomato)Hze/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. Double labeled: green - Crh; red - recombinase reporter.
Structure Level Pattern Image Note
TS28: central amygdaloid nucleus Present 4D Merge CeA , 4D Venus CeA

Specimen 4D - CTX: postnatal adult; Crhtm1.1(flpo)Bsab/Crhtm1.1Ksak, Gt(ROSA)26Sortm65.2(CAG-tdTomato)Hze/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. Double labeled: green - Crh; red - recombinase reporter.
Structure Level Pattern Image Note
TS28: cerebral cortex Present 4D Venus CTX , 4D Merge CTX

Specimen 4E - PVN: postnatal adult; Crhtm1.1Ksak/Crhtm1.1Ksak (more )
Note: Double labeled: green - Crh; red - recombinase reporter.
Structure Level Pattern Image Note
TS28: paraventricular hypothalamic nucleus Present 4E PVN

Specimen 4E - ME: postnatal adult; Crhtm1.1Ksak/Crhtm1.1Ksak (more )
Note: Double labeled: green - Crh; red - recombinase reporter.
Structure Level Pattern Image Note
TS28: median eminence Present 4E ME

Specimen 4E - IPACL: postnatal adult; Crhtm1.1Ksak/Crhtm1.1Ksak (more )
Note: Double labeled: green - Crh; red - recombinase reporter.
Structure Level Pattern Image Note
TS28: anterior commissure Present Regionally restricted 4E IPACL Expression is seen in the lateral part of the interstitial nucleus of the anterior commissure.

Specimen 4E - BNST: postnatal adult; Crhtm1.1Ksak/Crhtm1.1Ksak (more )
Note: Double labeled: green - Crh; red - recombinase reporter.
Structure Level Pattern Image Note
TS28: bed nucleus of stria terminalis Present 4E BNST

Specimen 4E - CeA: postnatal adult; Crhtm1.1Ksak/Crhtm1.1Ksak (more )
Note: Double labeled: green - Crh; red - recombinase reporter.
Structure Level Pattern Image Note
TS28: central amygdaloid nucleus Present 4E CeA

Specimen 4E - CTX: postnatal adult; Crhtm1.1Ksak/Crhtm1.1Ksak (more )
Note: Double labeled: green - Crh; red - recombinase reporter.
Structure Level Pattern Image Note
TS28: cerebral cortex Present 4E CTX


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last database update
03/25/2025
MGI 6.24
The Jackson Laboratory