Crh^tm1.1Ksak
Targeted Allele Detail

Summary

• Symbol: Crh^tm1.1Ksak
• Name: corticotropin releasing hormone; targeted mutation 1.1, Kenji Sakimura
• MGI ID: MGI:6144041
• Synonyms: CRF-VenusdeltaNeo, Crh^{tm1.1(venus)Ksak}
• Gene: Crh Location: Chr3:19747565-19749560 bp, - strand Genetic Position: Chr3, 5.75 cM
• Alliance: Crh^tm1.1Ksak page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:218341
• Parent Cell Line: RENKA (ES Cell)
• Strain of Origin: C57BL/6N

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutation: Insertion
• Mutation details: A Venus reporter gene and an FRT site flanked neomycin resistance gene cassette were inserted downstream of the translation initiation site in exon 2. The neo cassette was removed via subsequent Flp-mediated recombination. (J:104881, J:218341)

Expression

In Mice Carrying this Mutation:

18 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Crh Mutation: 39 strains or lines available

References

• Original: J:218341 Itoi K, et al., Visualization of corticotropin-releasing factor neurons by fluorescent proteins in the mouse brain and characterization of labeled neurons in the paraventricular nucleus of the hypothalamus. Endocrinology. 2014 Oct;155(10):4054-60
• All: 8 reference(s)