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Gene Expression Data
Assay Details
Assay
Reference: J:337132 Hiremath C, et al., Rap1 regulates lumen continuity via Afadin in renal epithelia. Dev Biol. 2023 Jun 3;501:20-27
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7539519
Gene symbol: Afdn
Gene name: afadin, adherens junction formation factor
Antibody: Anti-l/s-Afadin (A0224)
Detection system: Secondary antibody
Results
Specimen 5A control: embryonic day 14.5; Rap1atm1Morz/Rap1atm1Morz, Rap1btm1Morz/Rap1btm1Morz (more )
Note: Triple labeled: white - Afdn; purple - Muc1 (assay MGI:7539509); green - Ncam1 (assay MGI:7539508).
Structure Level Pattern Image Note
TS22: collecting duct Present 5A control right , 5A control left Expressed in apical lateral junctions.
TS22: s-shaped body Present 5A control right , 5A control left Expressed in apical lateral junctions.
Specimen 5A cKO: embryonic day 14.5; Rap1atm1Morz/Rap1atm1Morz, Rap1btm1Morz/Rap1btm1Morz, Tg(Six2-EGFP/cre)1Amc/0 (more )
Note: Conditional mutant. Triple labeled: white - Afdn; purple - Muc1 (assay MGI:7539509); green - Ncam1 (assay MGI:7539508).
Structure Level Pattern Image Note
TS22: s-shaped body Trace 5A cKO right , 5A cKO left Decreased expression compared with the controls.
TS22: collecting duct Present 5A cKO right , 5A cKO left Expressed in apical lateral junctions, similar as in the controls.
Specimen 5B control 2D: embryonic day 14.5; Rap1atm1Morz/Rap1atm1Morz, Rap1btm1Morz/Rap1btm1Morz (more )
Structure Cell Type Level Pattern Image Note
TS22: collecting duct kidney collecting duct epithelial cell Present 5B control 2D Expressed in apical lateral junctions.
TS22: renal vesicle Present 5B control 2D Expressed in apical lateral junctions.
Specimen 5B cKO 2D: embryonic day 14.5; Rap1atm1Morz/Rap1atm1Morz, Rap1btm1Morz/Rap1btm1Morz, Tg(Hoxb7-cre)13Amc/0 (more )
Note: Conditional mutant.
Structure Cell Type Level Pattern Image Note
TS22: collecting duct kidney collecting duct epithelial cell Weak 5B cKO 2D Clearly reduced expression at the apical lateral junctions.
TS22: renal vesicle Present 5B cKO 2D Similar as in the controls.
Specimen 5B control 3D: embryonic day 14.5; Rap1atm1Morz/Rap1atm1Morz, Rap1btm1Morz/Rap1btm1Morz (more )
Structure Cell Type Level Pattern Image Note
TS22: s-shaped body kidney collecting duct epithelial cell Present 5B control 3D left Expressed in apical lateral junctions.
TS22: renal vesicle Present 5B control 3D left Expressed in apical lateral junctions.
Specimen 5B cKO 3D: embryonic day 14.5; Rap1atm1Morz/Rap1atm1Morz, Rap1btm1Morz/Rap1btm1Morz, Tg(Hoxb7-cre)13Amc/0 (more )
Note: Conditional mutant.
Structure Cell Type Level Pattern Image Note
TS22: collecting duct kidney collecting duct epithelial cell Weak 5B cKO 3D left Clearly reduced expression at the apical lateral junctions.
TS22: renal vesicle Present 5B cKO 3D left Similar as in the controls.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/14/2024
MGI 6.23 		The Jackson Laboratory