Ncam1 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:337132 Hiremath C, et al., Rap1 regulates lumen continuity via Afadin in renal epithelia. Dev Biol. 2023 Jun 3;501:20-27
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:7539508
Gene symbol:	Ncam1
Gene name:	neural cell adhesion molecule 1

Antibody:	5B8 antibody
Detection system:	Secondary antibody

Results

Specimen 5A control: embryonic day 14.5; Rap1a^tm1Morz/Rap1a^tm1Morz, Rap1b^tm1Morz/Rap1b^tm1Morz (more

)
Note: Triple labeled: green - Ncam1; white - Afdn (assay MGI:7539519); purple - Muc1 (assay MGI:7539509).

Structure	Level	Pattern	Image	Note
TS22: s-shaped body	Present		5A control right	Expressed at lateral cell junctions.

5A cKO right ©

Specimen 5A cKO: embryonic day 14.5; Rap1a^tm1Morz/Rap1a^tm1Morz, Rap1b^tm1Morz/Rap1b^tm1Morz, Tg(Six2-EGFP/cre)1Amc/0 (more

)
Note: Conditional mutant. Triple labeled: green - Ncam1; white - Afdn (assay MGI:7539519); purple - Muc1 (assay MGI:7539509).

Structure	Level	Pattern	Image	Note
TS22: s-shaped body	Present		5A cKO right	Expressed at lateral cell junctions. Defects in lumen formation was seen.

Specimen S1 control: embryonic day 14.5; Rap1a^tm1Morz/Rap1a^tm1Morz, Rap1b^tm1Morz/Rap1b^tm1Morz (more

)
Note: Double labeled: green - Ncam1; red - Pard6b (assay MGI:7539579).

Structure	Level	Pattern	Image	Note
TS22: nephron epithelium	Present		S1_control right

S1_cKO right ©

Specimen S1 cKO: embryonic day 14.5; Rap1a^tm1Morz/Rap1a^tm1Morz, Rap1b^tm1Morz/Rap1b^tm1Morz, Tg(Six2-EGFP/cre)1Amc/0 (more

)
Note: Conditional mutant. Double labeled: green - Ncam1; red - Pard6b (assay MGI:7539579).

Structure	Level	Pattern	Image	Note
TS22: nephron epithelium	Present		S1_cKO right

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources Funding Information Warranty Disclaimer, Privacy Notice, Licensing, & Copyright Send questions and comments to User Support.	last database update 03/25/2025 MGI 6.24