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Gene Expression Data
Assay Details
Assay
Reference: J:337132 Hiremath C, et al., Rap1 regulates lumen continuity via Afadin in renal epithelia. Dev Biol. 2023 Jun 3;501:20-27
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7539503
Gene symbol: Pard6b
Gene name: par-6 family cell polarity regulator beta
Antibody: anti-PARD6B (H-64; sc-67392)
Detection system: Secondary antibody
Results
Specimen 4C 2D control: embryonic day 14.5; Rap1atm1Morz/Rap1atm1Morz, Rap1btm1Morz/Rap1btm1Morz (more )
Note: Double labeled: green - Pard6b; red - Ncam1 (assay MGI:7539505).
Structure Level Pattern Image Note
TS22: s-shaped body Present Regionally restricted 4C control 2D right , 4C control 2D left Expressed at the apical surface.
Specimen 4C 2D cKO: embryonic day 14.5; Rap1atm1Morz/Rap1atm1Morz, Rap1btm1Morz/Rap1btm1Morz, Tg(Six2-EGFP/cre)1Amc/0 (more )
Note: Conditional mutant. Double labeled: green - Pard6b; red - Ncam1 (assay MGI:7539505).
Structure Level Pattern Image Note
TS22: s-shaped body Present Regionally restricted 4C cKO 2D right , 4C cKO 2D left Expressed at the apical surface. Defects in lumen formation, with marked discontinuous lumens.
Specimen 4C 3D control: embryonic day 14.5; Rap1atm1Morz/Rap1atm1Morz, Rap1btm1Morz/Rap1btm1Morz (more )
Note: Double labeled: green - Pard6b; red - Ncam1 (assay MGI:7539505).
Structure Level Pattern Image Note
TS22: s-shaped body Present Regionally restricted 4C control 3D right , 4C control 3D left Expressed at the apical surface.
Specimen 4C 3D cKO: embryonic day 14.5; Rap1atm1Morz/Rap1atm1Morz, Rap1btm1Morz/Rap1btm1Morz, Tg(Six2-EGFP/cre)1Amc/0 (more )
Note: Conditional mutant. Double labeled: green - Pard6b; red - Ncam1 (assay MGI:7539505).
Structure Level Pattern Image Note
TS22: s-shaped body Present Regionally restricted 4C cKO 3D left , 4C cKO 3D right Expressed at the apical surface. Defects in lumen formation, with marked discontinuous lumens.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/23/2024
MGI 6.23 		The Jackson Laboratory