About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:337132 Hiremath C, et al., Rap1 regulates lumen continuity via Afadin in renal epithelia. Dev Biol. 2023 Jun 3;501:20-27
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7539505
Gene symbol: Ncam1
Gene name: neural cell adhesion molecule 1
Antibody: 5B8 antibody
Detection system: Secondary antibody
Results
Specimen 4C 2D control: embryonic day 14.5; Rap1atm1Morz/Rap1atm1Morz, Rap1btm1Morz/Rap1btm1Morz (more )
Note: Double labeled: red - Ncam1; green - Pard6b (assay MGI:7539503).
Structure Level Pattern Image Note
TS22: s-shaped body Present 4C control 2D right Expressed at lateral cell junctions.
Specimen 4C 2D cKO: embryonic day 14.5; Rap1atm1Morz/Rap1atm1Morz, Rap1btm1Morz/Rap1btm1Morz, Tg(Six2-EGFP/cre)1Amc/0 (more )
Note: Conditional mutant. Double labeled: red - Ncam1; green - Pard6b (assay MGI:7539503).
Structure Level Pattern Image Note
TS22: s-shaped body Present 4C cKO 2D right Expressed at lateral cell junctions. Defects in lumen formation, with marked discontinuous lumens. Some of the nephron tubules had abnormal morphogenesis, with abnormal shape, particularly at its tail.
Specimen 4C 3D control: embryonic day 14.5; Rap1atm1Morz/Rap1atm1Morz, Rap1btm1Morz/Rap1btm1Morz (more )
Note: Double labeled: red - Ncam1; green - Pard6b (assay MGI:7539503).
Structure Level Pattern Image Note
TS22: s-shaped body Present 4C control 3D right Expressed at lateral cell junctions.
Specimen 4C 3D cKO: embryonic day 14.5; Rap1atm1Morz/Rap1atm1Morz, Rap1btm1Morz/Rap1btm1Morz, Tg(Six2-EGFP/cre)1Amc/0 (more )
Note: Conditional mutant. Double labeled: red - Ncam1; green - Pard6b (assay MGI:7539503).
Structure Level Pattern Image Note
TS22: s-shaped body Present 4C cKO 3D right Expressed at lateral cell junctions. Defects in lumen formation, with marked discontinuous lumens. Some of the nephron tubules had abnormal morphogenesis, with abnormal shape, particularly at its tail.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
03/18/2025
MGI 6.24 		The Jackson Laboratory