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Gene Expression Data
Assay Details
Assay
Reference: J:321979 Kurz J, et al., Notch signaling is a novel regulator of visceral smooth muscle cell differentiation in the murine ureter. Development. 2022 Feb 15;149(4):dev199735
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7539424
Gene symbol: Notch3
Gene name: notch 3
Antibody: Anti-Notch3 (ab23426)
Detection system: Secondary antibody coupled to Alexa Fluor 555
Results
Specimen 2C control: embryonic day 18.5; Rbpjtm1Hon/? (more )
Note: Controls were littermates with no cre.
Structure Cell Type Level Pattern Image Note
TS26: ureteral smooth muscle layer ureter smooth muscle cell Present Regionally restricted 2C Notch3 control Expression was detected in smooth muscle cells of the tunica muscularis.
TS26: ureteral vasculature vascular associated smooth muscle cell Present Regionally restricted 2C Notch3 control Expression was detected in large adventitial vessels.
Specimen 2C Rbpj-cKO: embryonic day 18.5; Rbpjtm1Hon/Rbpjtm1Hon, Tbx18tm4(cre)Akis/Tbx18+ (more )
Note: Conditional mutant.
Structure Cell Type Level Pattern Image Note
TS26: ureteral smooth muscle layer ureter smooth muscle cell Present Regionally restricted 2C Notch3 Rbpj-cKO Expression was unchanged in the tunica muscularis of the mutant.
TS26: ureteral vasculature vascular associated smooth muscle cell Weak Regionally restricted 2C Notch3 Rbpj-cKO Expression was reduced in large adventitial vessels of the mutant.
Specimen 3B control: postnatal day 4; Rbpjtm1Hon/? (more )
Note: Controls were littermates with no cre.
Structure Cell Type Level Pattern Image Note
TS28: ureteral smooth muscle layer ureter smooth muscle cell Present 3B control Notch3
Specimen 3B Rbpj-cKO: postnatal day 4; Rbpjtm1Hon/Rbpjtm1Hon, Tbx18tm4(cre)Akis/Tbx18+ (more )
Note: Conditional mutant.
Structure Cell Type Level Pattern Image Note
TS28: ureteral smooth muscle layer ureter smooth muscle cell Present 3B Rbpj-cKO Notch3 Expression was unchanged in the mutant compared with control.
Specimen 4B E14.5 control: embryonic day 14.5; Rbpjtm1Hon/? (more )
Note: Controls were littermates with no cre.
Structure Level Pattern Image Note
TS22: ureteral mesenchyme Absent 4B control E14.5 Notch3
Specimen 4B E14.5 Rbpj-cKO: embryonic day 14.5; Rbpjtm1Hon/Rbpjtm1Hon, Tbx18tm4(cre)Akis/Tbx18+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS22: ureteral mesenchyme Absent 4B Rbpj-cKO E14.5 Notch3
Specimen 4B E15.5 control: embryonic day 15.5; Rbpjtm1Hon/? (more )
Note: Controls were littermates with no cre.
Structure Level Pattern Image Note
TS23: ureteral mesenchyme Absent 4B control E15.5 Notch3
Specimen 4B E15.5 Rbpj-cKO: embryonic day 15.5; Rbpjtm1Hon/Rbpjtm1Hon, Tbx18tm4(cre)Akis/Tbx18+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS23: ureteral mesenchyme Absent 4B Rbpj-cKO E15.5 Notch3
Specimen 4B E16.5 control: embryonic day 16.5; Rbpjtm1Hon/? (more )
Note: Controls were littermates with no cre.
Structure Level Pattern Image Note
TS24: ureteral mesenchyme Present Regionally restricted 4B control E16.5 Notch3 Expression commenced at E16.5 in the inner layer of the ureteric mesenchyme.
Specimen 4B E16.5 Rbpj-cKO: embryonic day 16.5; Rbpjtm1Hon/Rbpjtm1Hon, Tbx18tm4(cre)Akis/Tbx18+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS24: ureteral mesenchyme Absent 4B Rbpj-cKO E16.5 Notch3
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/07/2024
MGI 6.23 		The Jackson Laboratory