Ccn2 RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:165111 Doherty HE, et al., A mouse strain where basal connective tissue growth factor gene expression can be switched from low to high. PLoS One. 2010;5(9):e12909
Assay type:	RT-PCR
MGI Accession ID:	MGI:7539399
Gene symbol:	Ccn2
Gene name:	cellular communication network factor 2

Probe:	Ctgf-pF, Ctgf-pR
Assay notes:	Quantitative RT-PCR. Relative expression was normalized to was normalized to a combined control of animals with the genotypes: +/+;Cre-, +/+;Cre+, and Lo/+;Cre-.

Results

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	Size Not Specified	Amount	Genetic Background	Mutant Allele(s)	Sex	Note
Heart Control	P m 10	TS28: heart	Present	Not Specified	involves: 129S6/SvEvTac * C57BL/6 * FVB/N		Not Specified	Control could be any of these genotypes: +/+;Cre-; +/+;Cre+, or Lo/+;Cre-.
Heart Hi/+	P m 10	TS28: heart	Present (a)	Not Specified	involves: 129S6/SvEvTac * C57BL/6 * FVB/N	Ccn2^tm2Mae/Ccn2⁺, Tg(EIIa-cre)C5379Lmgd/?	Not Specified	Conditional mutant.
Kidney Control	P m 10	TS28: metanephros	Present	Not Specified	involves: 129S6/SvEvTac * C57BL/6 * FVB/N		Not Specified	Control could be any of these genotypes: +/+;Cre-; +/+;Cre+, or Lo/+;Cre-.
Kidney Hi/+	P m 10	TS28: metanephros	Present (b)	Not Specified	involves: 129S6/SvEvTac * C57BL/6 * FVB/N	Ccn2^tm2Mae/Ccn2⁺, Tg(EIIa-cre)C5379Lmgd/?	Not Specified	Conditional mutant.
Lung Control	P m 10	TS28: lung	Present	Not Specified	involves: 129S6/SvEvTac * C57BL/6 * FVB/N		Not Specified	Control could be any of these genotypes: +/+;Cre-; +/+;Cre+, or Lo/+;Cre-.
Lung Hi/+	P m 10	TS28: lung	Present (c)	Not Specified	involves: 129S6/SvEvTac * C57BL/6 * FVB/N	Ccn2^tm2Mae/Ccn2⁺, Tg(EIIa-cre)C5379Lmgd/?	Not Specified	Conditional mutant.

Notes:
(a) There was a significant increase in expression in each tissue of Hi/+ animals. Heart expression was two-fold above controls.
(b) There was a significant increase in expression in each tissue of Hi/+ animals. Kidney expression was three-fold above controls.
(c) There was a significant increase in expression in each tissue of Hi/+ animals. Lung expression was nearly five-fold above controls.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources Funding Information Warranty Disclaimer, Privacy Notice, Licensing, & Copyright Send questions and comments to User Support.	last database update 03/25/2025 MGI 6.24