Antibody:	anti-HO-2 (ADI-SPA-897)
Detection system:	Secondary antibody coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS20: embryo	Strong	Widespread	2A	Intense expression observed throughout section, similar to that of Hmox1

Structure	Level	Pattern	Image	Note
TS20: embryo	Strong	Widespread	2B	Intense expression observed throughout section, similar to that of Hmox1

Structure	Level	Pattern	Image	Note
TS20: embryo	Present	Widespread	2C	Pattern of expression similar to that of wWT.

Structure	Level	Pattern	Image	Note
TS20: embryo	Present	Widespread	2D	Pattern of expression similar to that of wWT.

Structure	Level	Pattern	Image	Note
TS20: embryo	Weak		2E	Expression less intense compared to wWT and WT.

Structure	Level	Pattern	Image	Note
TS20: embryo	Weak		2F	Expression less intense compared to wWT and WT.

Structure	Level	Pattern	Image	Note
TS20: corpus striatum	Present	Regionally restricted	2G	Expression in cytoplasm and nucleus of brain cells.

Structure	Level	Pattern	Image	Note
TS20: corpus striatum	Present	Regionally restricted	2H	Expression in cytoplasm of brain similar but less nuclear expression compared to that of wWT.

Structure	Level	Pattern	Image	Note
TS20: corpus striatum	Present	Regionally restricted	2I	Both cytoplasmic and nuclear expression low compared with wWT and WT.

Structure	Cell Type	Level	Pattern	Image	Note
TS20: cardiac muscle tissue	cardiac muscle cell	Present		2J

Structure	Cell Type	Level	Pattern	Image	Note
TS20: cardiac muscle tissue	cardiac muscle cell	Present		2K	Expression in cardiac muscle cells slightly higher compared to wWT.

Structure	Level	Pattern	Image	Note
TS20: cardiac muscle tissue	Weak		2L

Structure	Cell Type	Level	Pattern	Image	Note
TS20: lung epithelium	epithelial cell of lung	Present	Regionally restricted	2M	Expression in cytoplasm and nuclei of epithelial cells.
TS20: lung mesenchyme		Present	Regionally restricted	2M	Expression in cytoplasm and nuclei of mesenchymal cells.

Structure	Cell Type	Level	Pattern	Image	Note
TS20: lung epithelium	epithelial cell of lung	Present	Regionally restricted	2N	Expression in cytoplasm and nuclei of epithelial cells slightly low compared to that of wWT.
TS20: lung mesenchyme		Present	Regionally restricted	2N	Expression in cytoplasm and nuclei of mesenchymal cells slightly low compared to that of wWT.

Structure	Cell Type	Level	Pattern	Image	Note
TS20: lung epithelium	epithelial cell of lung	Present	Regionally restricted	2O	Expression in cytoplasm and nuclei of epithelial cells slightly low compared to that of wWT.
TS20: lung mesenchyme		Present	Regionally restricted	2O	Expression in cytoplasm and nuclei of mesenchymal cells slightly low compared to that of wWT.

Structure	Level	Pattern	Image	Note
TS20: liver parenchyma	Present	Regionally restricted	2P	Cytoplasmic and nuclear expression in liver.

Structure	Level	Pattern	Image	Note
TS20: liver parenchyma	Present	Regionally restricted	2Q	Cytoplasmic and nuclear expression in liver comparable to that of wWT.

Structure	Level	Pattern	Image	Note
TS20: liver parenchyma	Weak		2R	Expression low compared to that of wWT.

Structure	Level	Pattern	Image	Note
TS26: embryo	Present	Ubiquitous	4A	Uniform expression throughout embryo.
TS26: liver	Strong		4A	Intense expression in all organs visible at this age.
TS26: lung	Strong		4A	Intense expression in all organs visible at this age.
TS26: metanephros	Strong		4A	Intense expression in all organs visible at this age.

Structure	Level	Pattern	Image	Note
TS26: embryo	Present	Ubiquitous	4B	Uniform expression throughout embryo.
TS26: forebrain	Strong		4B	Intense expression in all organs visible at this age.
TS26: heart	Strong		4B	Intense expression in all organs visible at this age.

Structure	Level	Pattern	Image	Note
TS26: embryo	Present	Ubiquitous	4C	Expression comparable to that of wWT but intensity was slightly lower
TS26: liver	Present		4C
TS26: lung	Present		4C
TS26: metanephros	Present		4C

Structure	Level	Pattern	Image	Note
TS26: embryo	Present	Ubiquitous	4D	Expression comparable to that of wWT but intensity was slightly lower
TS26: forebrain	Present		4D
TS26: heart	Present		4D

Structure	Level	Pattern	Image	Note
TS26: embryo	Weak		4E	Expression markedly lower compared with wWT and WT.

Structure	Level	Pattern	Image	Note
TS26: embryo	Weak		4F	Expression markedly lower compared with wWT and WT.

Structure	Level	Pattern	Image	Note
TS26: forebrain	Strong	Regionally restricted	A3G	Expression localized uniforrmly in cytoplasm and in nucleus of most cells.

Structure	Level	Pattern	Image	Note
TS26: forebrain	Present	Regionally restricted	A3H	Intensity of expression in cytoplasm and nucleus of brain cells: wWT>WT>KO.

Structure	Level	Pattern	Image	Note
TS26: forebrain	Weak	Regionally restricted	A3I	Intensity of expression in cytoplasm and nucleus of brain cells: wWT>WT>KO.

Structure	Cell Type	Level	Pattern	Image	Note
TS26: cardiac muscle tissue	cardiac muscle cell	Present	Regionally restricted	A3J	Expression throughout cytoplasm of cardiac muscle cells but in nucleus of only a few cells.

Structure	Cell Type	Level	Pattern	Image	Note
TS26: cardiac muscle tissue	cardiac muscle cell	Present	Regionally restricted	A3K	Intensity of expression in cytoplasm and nucleus of cardiac muscle cells: wWT>WT>KO.

Structure	Cell Type	Level	Pattern	Image	Note
TS26: cardiac muscle tissue	cardiac muscle cell	Present	Regionally restricted	A3L	Intensity of expression in cytoplasm and nucleus of cardiac muscle cells: wWT>WT>KO.

Structure	Level	Pattern	Image	Note
TS26: bronchiole epithelium	Strong	Regionally restricted	4G	Intense expression in cytoplasm and nucleus of most cell surrounding alveoli and epithelial cells of bronchioles.
TS26: lung alveolus	Strong	Regionally restricted	4G	Intense expression in cytoplasm and nucleus of most cell surrounding alveoli and epithelial cells of bronchioles.

Structure	Level	Pattern	Image	Note
TS26: bronchiole epithelium	Present		4H	Expression lower than for wWT.
TS26: lung alveolus	Present		4H	Expression lower than for wWT.

Structure	Level	Pattern	Image	Note
TS26: bronchiole epithelium	Present		4I	Expression lower than for wWT.
TS26: lung alveolus	Present		4I	Expression lower than for wWT.

Structure	Level	Pattern	Image	Note
TS26: liver parenchyma	Strong	Regionally restricted	4J	Intense and uniform expression in cytoplasm of liver parenchymal cells. Nuclear expression in a few cells.

Structure	Level	Pattern	Image	Note
TS26: liver parenchyma	Present		4K	Lower expression than for wWT.

Structure	Level	Pattern	Image	Note
TS26: liver parenchyma	Present		4L	Lower expression than for wWT.

Structure	Level	Pattern	Image	Note
TS26: distal convoluted tubule	Strong	Regionally restricted	4M	Intense cytoplasmic expression in cortical region, including proximal and distal convoluted tubules.
TS26: renal proximal convoluted tubule	Strong	Regionally restricted	4M	Intense cytoplasmic expression in cortical region, including proximal and distal convoluted tubules.
TS26: glomerular tuft	Weak		4M	Faint expression in glomeruli.

Structure	Level	Pattern	Image	Note
TS26: distal convoluted tubule	Weak		4N	Low expression in tubules.
TS26: renal proximal convoluted tubule	Weak		4N	Low expression in tubules.
TS26: glomerular tuft	Weak		4N	Faint expression in glomeruli.

Structure	Level	Pattern	Image	Note
TS26: distal convoluted tubule	Weak		4O	Low expression in tubules.
TS26: renal proximal convoluted tubule	Weak		4O	Low expression in tubules.

Structure	Level	Pattern	Image	Note
TS26: collecting duct	Strong	Regionally restricted	4P	Intense cytoplasmic expression in medullary region, including collecting ducts and loop of Henle.
TS26: loop of Henle	Strong	Regionally restricted	4P	Intense cytoplasmic expression in medullary region, including collecting ducts and loop of Henle.

Structure	Level	Pattern	Image	Note
TS26: collecting duct	Weak		4Q	Low expression in tubules.
TS26: loop of Henle	Weak		4Q	Low expression in tubules.

Structure	Level	Pattern	Image	Note
TS26: collecting duct	Weak		4R	Low expression in tubules.
TS26: loop of Henle	Weak		4R	Low expression in tubules.