Antibody:	HO-1 antibody (SPA-895)
Detection system:	Secondary antibody coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS20: embryo	Present	Widespread	1B	Expression throughout embryo
TS20: corpus striatum	Strong		1B	Intense expression in corpus striatum, wall of midbrain, tongue, mantle layer of spinal cord and liver
TS20: liver	Strong		1B	Intense expression in corpus striatum, wall of midbrain, tongue, mantle layer of spinal cord and liver
TS20: midbrain lateral wall	Strong		1B	Intense expression in corpus striatum, wall of midbrain, tongue, mantle layer of spinal cord and liver
TS20: spinal cord mantle layer	Strong		1B	Intense expression in corpus striatum, wall of midbrain, tongue, mantle layer of spinal cord and liver
TS20: tongue	Strong		1B	Intense expression in corpus striatum, wall of midbrain, tongue, mantle layer of spinal cord and liver
TS20: neocortex	Strong	Regionally restricted	1B	Intense expression in roof of neopallial cortex

Structure	Level	Pattern	Image	Note
TS20: embryo	Present	Widespread	1C	Expression throughout embryo.
TS20: dorsal root ganglion	Strong		1C	Intense expression in medulla oblongata, tongue, dorsal root ganglion and mantle layer of spinal cord
TS20: medulla oblongata	Strong		1C	Intense expression in medulla oblongata, tongue, dorsal root ganglion and mantle layer of spinal cord
TS20: spinal cord mantle layer	Strong		1C	Intense expression in medulla oblongata, tongue, dorsal root ganglion and mantle layer of spinal cord
TS20: tongue	Strong		1C	Intense expression in medulla oblongata, tongue, dorsal root ganglion and mantle layer of spinal cord

Structure	Level	Pattern	Image	Note
TS20: embryo	Present	Widespread	1E	Expression similar to that of wWT embryos.
TS20: midbrain roof plate	Present		1E	Expression slightly high in neopallial cortex, roof of midbrain and mantle layer of spinal cord
TS20: neocortex	Present		1E	Expression slightly high in neopallial cortex, roof of midbrain and mantle layer of spinal cord
TS20: spinal cord mantle layer	Present		1E	Expression slightly high in neopallial cortex, roof of midbrain and mantle layer of spinal cord

Structure	Level	Pattern	Image	Note
TS20: embryo	Present	Widespread	1F	Expression similar to that of wWT embryos.
TS20: medulla oblongata	Present		1F	Expression slightly high in medulla oblongata

Structure	Level	Pattern	Image	Note
TS20: embryo	Absent		1H

Structure	Level	Pattern	Image	Note
TS20: embryo	Absent		1I

Structure	Level	Pattern	Image	Note
TS20: corpus striatum	Present	Regionally restricted	1J	Expression in cytoplasm and nucleus of brain cells.

Structure	Level	Pattern	Image	Note
TS20: corpus striatum	Present	Regionally restricted	1K	Expression slightly lower compared to that of wWT, although nuclear expression appears comparable.

Structure	Cell Type	Level	Pattern	Image	Note
TS20: cardiac muscle tissue	cardiac muscle cell	Present	Regionally restricted	1L	Expression in cytoplasm and nucleus of cardiac muscle cells.

Structure	Cell Type	Level	Pattern	Image	Note
TS20: cardiac muscle tissue	cardiac muscle cell	Present	Regionally restricted	1M	Expression in cytoplasm and nucleus of cardiac muscle cells comparable to that of wWT.

Structure	Cell Type	Level	Pattern	Image	Note
TS20: lung epithelium	epithelial cell of lung	Present	Regionally restricted	1N	Expression in cytoplasm of epithelial cells of lung bud; only some of cells showed nuclear expression.
TS20: lung mesenchyme		Present	Regionally restricted	1N	Expression in cytoplasm of surrounding mesenchymal cells; only some of cells showed nuclear expression.

Structure	Level	Pattern	Image	Note
TS20: lung epithelium	Present	Regionally restricted	1O	Expression similar to that of wWT but with lower intensity.
TS20: lung mesenchyme	Present	Regionally restricted	1O	Expression similar to that of wWT but with lower intensity.

Structure	Level	Pattern	Image	Note
TS20: liver parenchyma	Present	Regionally restricted	1P	Expression in cytoplasm and nucleus of most cells in liver parenchyma.

Structure	Level	Pattern	Image	Note
TS20: liver parenchyma	Present	Regionally restricted	1Q	Expression in cytoplasm and nucleus of cells in liver parenchyma, slightly less compared to wWT.

Structure	Level	Pattern	Image	Note
TS26: embryo	Present	Regionally restricted	3B	Authors report differential expression patterns.
TS26: liver	Strong		3B	High expression in liver.
TS26: metanephros	Moderate		3B	Moderate expression in kidney.
TS26: lung	Weak		3B	Low expression in lung.

Structure	Level	Pattern	Image	Note
TS26: embryo	Present	Regionally restricted	3C	Authors report differential expression patterns.
TS26: forebrain	Strong	Regionally restricted	3C	High expression in all regions of brain. Uniform expression in cytoplasm of brain cells. Nuclear expression in some cells of forebrain.
TS26: hindbrain	Strong	Regionally restricted	3C	High expression in all regions of brain. Uniform expression in cytoplasm of brain cells. Nuclear expression in some cells of forebrain.
TS26: midbrain	Strong	Regionally restricted	3C	High expression in all regions of brain. Uniform expression in cytoplasm of brain cells. Nuclear expression in some cells of forebrain.
TS26: brown fat	Strong		3C	High expression in heart, deposits of brown fat and mantle layer of spinal cord.
TS26: heart	Strong		3C	High expression in heart, deposits of brown fat and mantle layer of spinal cord.
TS26: spinal cord mantle layer	Strong		3C	High expression in heart, deposits of brown fat and mantle layer of spinal cord.
TS26: forelimb	Moderate		3C	Moderate expression in tongue and forelimbs.
TS26: tongue	Moderate		3C	Moderate expression in tongue and forelimbs.
TS26: thymus	Weak		3C	Low expression in thymus gland.

Structure	Level	Pattern	Image	Note
TS26: embryo	Present	Regionally restricted	3E	Differential pattern of expression, but overall intensity slightly low compared to that of wWT.

Structure	Level	Pattern	Image	Note
TS26: embryo	Present	Regionally restricted	3F	Differential pattern of expression, but overall intensity slightly low compared to that of wWT.

Structure	Level	Pattern	Image	Note
TS26: embryo	Absent		3H

Structure	Level	Pattern	Image	Note
TS26: embryo	Absent		3I

Structure	Level	Pattern	Image	Note
TS26: forebrain	Present	Regionally restricted	A3A	Uniform expression in cytoplasm of brain cells. Nuclear expression in some cells of forebrain.

Structure	Level	Pattern	Image	Note
TS26: forebrain	Present	Regionally restricted	A3B	Cytoplasmic and nuclear expression low compared to wWT.

Structure	Level	Pattern	Image	Note
TS26: forebrain	Absent		A3C

Structure	Cell Type	Level	Pattern	Image	Note
TS26: cardiac muscle tissue	cardiac muscle cell	Present	Regionally restricted	A3D	Expression in cardiac muscle cells throughout cytoplasm. Nuclear expression only in a few cells.

Structure	Cell Type	Level	Pattern	Image	Note
TS26: cardiac muscle tissue	cardiac muscle cell	Present	Regionally restricted	A3E	Expression in cardiac muscle cells lower than in wWT.

Structure	Level	Pattern	Image	Note
TS26: heart	Absent		A3F

Structure	Level	Pattern	Image	Note
TS26: bronchiole epithelium	Present	Regionally restricted	3J	Moderate expression in nucleus of most cells surrounding alveoli and epithelial cells of bronchioles. Cytoplasmic expression in most cells but intensity low.
TS26: lung alveolus	Present	Regionally restricted	3J	Moderate expression in nucleus of most cells surrounding alveoli and epithelial cells of bronchioles. Cytoplasmic expression in most cells but intensity low.

Structure	Level	Pattern	Image	Note
TS26: bronchiole epithelium	Present	Regionally restricted	3K	Expression comparable to that of wWT.
TS26: lung alveolus	Present	Regionally restricted	3K	Expression comparable to that of wWT.

Structure	Level	Pattern	Image	Note
TS26: liver parenchyma	Present	Regionally restricted	3L	Expression in both cytoplasm and nucleus of some parenchymal cells. Intense expression in small clusters.

Structure	Level	Pattern	Image	Note
TS26: liver parenchyma	Present	Regionally restricted	3M	Expression similar to that of wWT.

Structure	Level	Pattern	Image	Note
TS26: distal convoluted tubule	Present	Regionally restricted	3N	Expression in cytoplasm and nucleus of most proximal and distal convoluted tubules.
TS26: renal proximal convoluted tubule	Present	Regionally restricted	3N	Expression in cytoplasm and nucleus of most proximal and distal convoluted tubules.
TS26: glomerular tuft	Weak		3N	Faint expression in glomeruli.
TS26: renal interstitium	Weak		3N	Faint cytoplasmic expression in a few interstitial cells of parenchyma.

Structure	Level	Pattern	Image	Note
TS26: distal convoluted tubule	Present	Regionally restricted	3O	Expression in cytoplasm and nuclei of some proximal and distal tubules.
TS26: renal proximal convoluted tubule	Present	Regionally restricted	3O	Expression in cytoplasm and nuclei of some proximal and distal tubules.

Structure	Level	Pattern	Image	Note
TS26: collecting duct	Present	Regionally restricted	3P	Cytoplasmic and nuclear expression in most cells of collecting ducts and loop of Henle.
TS26: loop of Henle	Present	Regionally restricted	3P	Cytoplasmic and nuclear expression in most cells of collecting ducts and loop of Henle.
TS26: renal interstitium	Weak		3P	Faint cytoplasmic expression in a few interstitial cells of parenchyma.

Structure	Level	Pattern	Image	Note
TS26: collecting duct	Present		3Q	Expression in collecting ducts, comparable to that of wWT.