Sox9 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:238708 Gonen N, et al., Normal Levels of Sox9 Expression in the Developing Mouse Testis Depend on the TES/TESCO Enhancer, but This Does Not Act Alone. PLoS Genet. 2017 Jan;13(1):e1006520
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:7536988
Gene symbol:	Sox9
Gene name:	SRY (sex determining region Y)-box 9

Antibody:	anti-Sox9
Detection system:	Secondary antibody coupled to Alexa Fluor 488

Results

2C 6W XY wild type Merged (+Dapi) ©

2C 6W XY wild type Sox9 ©

Specimen 2C wild type: postnatal week 6 (more

)
Note: Double labeled: green - Sox9; magenta - Foxl2 (assay MGI:7536997).

Structure	Cell Type	Level	Pattern	Image	Note
TS28: Sertoli cell	Sertoli cell	Present		2C 6W XY wild type Merged (+Dapi) , 2C 6W XY wild type Sox9

2C 6W XY Tesco-/- Merged (+Dapi) ©

2C 6W XY Tesco-/- Sox9 ©

Specimen 2C TESCO-/-: postnatal week 6; Sox9^em1Rlb/Sox9^em1Rlb (more

)
Note: Double labeled: green - Sox9; magenta - Foxl2 (assay MGI:7536997).

Structure	Cell Type	Level	Pattern	Image	Note
TS28: Sertoli cell	Sertoli cell	Present		2C 6W XY Tesco-/- Merged (+Dapi) , 2C 6W XY Tesco-/- Sox9	Staining in the TESCO-/- mutant was indistinguishable from wild type testes.

3B E13.5 XY wild type Merged (+Dapi) ©

3B E13.5 XY wild type Sox9 ©

Specimen 3B wild type: embryonic day 13.5 (more

)
Note: Double labeled: green - Sox9; red - Foxl2 (assay MGI:7536997).

Structure	Level	Pattern	Image	Note
TS21: testis	Present		3B E13.5 XY wild type Sox9 , 3B E13.5 XY wild type Merged (+Dapi)

3B E13.5 XY Tes-/- Merged (+Dapi) ©

3B E13.5 XY Tes-/- Sox9 ©

Specimen 3B TES-/-: embryonic day 13.5; Sox9^em3Rlb/Sox9^em3Rlb (more

)
Note: Double labeled: green - Sox9; red - Foxl2 (assay MGI:7536997).

Structure	Level	Pattern	Image	Note
TS21: testis	Present		3B E13.5 XY Tes-/- Merged (+Dapi) , 3B E13.5 XY Tes-/- Sox9	Staining in the TES-/- mutant was indistinguishable from wild type testes.

3C 8W XY wild type Merged (+Dapi) ©

3C 8W XY wild type Sox9 ©

Specimen 3C wild type: postnatal week 8 (more

)
Note: Double labeled: green - Sox9; red - Foxl2 (assay MGI:7536997).

Structure	Level	Pattern	Image	Note
TS28: testis	Present		3C 8W XY wild type Sox9 , 3C 8W XY wild type Merged (+Dapi)

3C 8W XY Tes-/- Merged (+Dapi) ©

3C 8W XY Tes-/- Sox9 ©

Specimen 3C TES-/-: postnatal week 8; Sox9^em3Rlb/Sox9^em3Rlb (more

)
Note: Double labeled: green - Sox9; red - Foxl2 (assay MGI:7536997).

Structure	Level	Pattern	Image	Note
TS28: testis	Present		3C 8W XY Tes-/- Merged (+Dapi) , 3C 8W XY Tes-/- Sox9	Staining intensity appeared slightly lower in the TES mutant compared with wild type, but authors indicated this was speculative.

5A E14.5 XY TES+/+Sox8-/- Merged (+Dapi) ©

5A E14.5 XY TES+/+Sox8-/- Sox9 ©

Specimen 5A top: embryonic day 14.5; Sox8^tm1Weg/Sox8^tm1Weg (more

)

Structure	Level	Pattern	Image	Note
TS22: testis	Present		5A E14.5 XY TES+/+Sox8-/- Sox9 , 5A E14.5 XY TES+/+Sox8-/- Merged (+Dapi)

5A E14.5 XY Tes-/-Sox8-/- Merged (+Dapi) ©

5A E14.5 XY Tes-/-Sox8-/- Sox9 ©

Specimen 5A bottom: embryonic day 14.5; Sox8^tm1Weg/Sox8^tm1Weg, Sox9^em3Rlb/Sox9^em3Rlb (more

)

Structure	Level	Pattern	Image	Note
TS22: testis	Present		5A E14.5 XY Tes-/-Sox8-/- Sox9 , 5A E14.5 XY Tes-/-Sox8-/- Merged (+Dapi)	Expression was retained in the double mutant.

5B 8W XY TES+/+Sox8-/- Merged (+Dapi) ©

5B 8W XY TES+/+Sox8-/- Sox9 ©

Specimen 5B top: postnatal week 8; Sox8^tm1Weg/Sox8^tm1Weg (more

)

Structure	Level	Pattern	Image	Note
TS28: testis	Present		5B 8W XY TES+/+Sox8-/- Sox9 , 5B 8W XY TES+/+Sox8-/- Merged (+Dapi)

5B 8W XY Tes-/-Sox8-/- Merged (+Dapi) ©

5B 8W XY Tes-/-Sox8-/- Sox9 ©

Specimen 5B bottom: postnatal week 8; Sox8^tm1Weg/Sox8^tm1Weg, Sox9^em3Rlb/Sox9^em3Rlb (more

)

Structure	Level	Pattern	Image	Note
TS28: testis	Present		5B 8W XY Tes-/-Sox8-/- Merged (+Dapi) , 5B 8W XY Tes-/-Sox8-/- Sox9	Expression was retained in the double mutant.

S2C 6W XX wild type Merged (+Dapi) ©

S2C 6W XX wild type Sox9 ©

Specimen S2C wild type: postnatal week 6 (more

)
Note: Double labeled: green - Sox9; magenta - Foxl2 (assay MGI:7536997).

Structure	Level	Pattern	Image	Note
TS28: ovary	Absent		S2C 6W XX wild type Merged (+Dapi) , S2C 6W XX wild type Sox9

Specimen S2C TESCO-/-: postnatal week 6; Sox9^em1Rlb/Sox9^em1Rlb (more

)
Note: Double labeled: green - Sox9; magenta - Foxl2 (assay MGI:7536997).

Structure	Level	Pattern	Image	Note
TS28: ovary	Absent		S2C 6W XX Tesco-/- Merged (+Dapi) , S2C 6W XX Tesco-/- Sox9	As in wild type, staining was not detected in the mutant ovary.

Specimen S4A wild type: embryonic day 13.5 (more

)
Note: Double labeled: green - Sox9; red - Foxl2 (assay MGI:7536997).

Structure	Level	Pattern	Image	Note
TS21: ovary	Absent		S4A E13.5 XX wild type Merged (+Dapi) , S4A E13.5 XX wild type Sox9

Specimen S4A TES-/-: embryonic day 13.5; Sox9^em3Rlb/Sox9^em3Rlb (more

)
Note: Double labeled: green - Sox9; red - Foxl2 (assay MGI:7536997).

Structure	Level	Pattern	Image	Note
TS21: ovary	Absent		S4A E13.5 XX Tes-/- Sox9 , S4A E13.5 XX Tes-/- Merged (+Dapi)	As in wild type, staining was not detected in the mutant ovary.

Specimen S4B wild type: postnatal week 8 (more

)
Note: Double labeled: green - Sox9; red - Foxl2 (assay MGI:7536997).

Structure	Level	Pattern	Image	Note
TS28: ovary	Absent		S4B 8W XX wild type Merged (+Dapi) , S4B 8W XX wild type Sox9

Specimen S4B TES-/-: postnatal week 8; Sox9^em3Rlb/Sox9^em3Rlb (more

)
Note: Double labeled: green - Sox9; red - Foxl2 (assay MGI:7536997).

Structure	Level	Pattern	Image	Note
TS28: ovary	Absent		S4B 8W XX Tes-/- Merged (+Dapi) , S4B 8W XX Tes-/- Sox9	As in wild type, staining was not detected in the mutant ovary.

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