About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:93831 Ebert SN, et al., Targeted insertion of the Cre-recombinase gene at the phenylethanolamine n-methyltransferase locus: A new model for studying the developmental distribution of adrenergic cells. Dev Dyn. 2004 Dec;231(4):849-58
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7536969
Gene symbol: Hcn4
Gene name: hyperpolarization-activated, cyclic nucleotide-gated K+ 4
Antibody: anti-HCN4 (AB5808)
Detection system: Secondary antibody coupled to fluorescein
Results
Specimen 7A: embryonic day 15.5 (more )
Structure Level Pattern Image Note
TS23: sinoatrial node Strong 7A Highly restricted to the sinoatrial node (SAN) region of the right atrium.
TS23: impulse conducting system Present Regionally restricted 7A Expressed in a lesser extent in the atrioventricular node (AVN) region.
Specimen 7G/H: embryonic day 15.5; Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+, Pnmttm1(cre)Sne/Pnmt+ (more )
Note: Conditional mutant. Overlay by merging the individual staining panels: green - Hcn4; blue - Pnmt reporter.
Structure Level Pattern Image Note
TS23: sinoatrial node Strong 7H , 7G Overlapped extensively with the XGAL staining.
Specimen 8C/D: postnatal day 2; Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+, Pnmttm1(cre)Sne/Pnmt+ (more )
Note: Conditional mutant. Overlay by merging the individual staining panels: green - Hcn4; blue - Pnmt reporter.
Structure Level Pattern Image Note
TS27: sinoatrial node Present 8C , 8D Many of the pacemaker cells (Hcn4+) were also stained blue with XGAL.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
03/25/2025
MGI 6.24 		The Jackson Laboratory