Gene Expression Data
Assay Details
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Assay
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Reference:
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J:268103
Dykes IM, et al., A Requirement for Zic2 in the Regulation of Nodal Expression Underlies the Establishment of Left-Sided Identity. Sci Rep. 2018 Jul 11;8(1):10439
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:7530985
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Gene symbol:
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Nodal
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Gene name:
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nodal growth differentiation factor
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Probe:
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Nodal probe
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Results
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Specimen
4i:
embryonic day 8.0
(more  )
Note: 4-somite stage
Specimen
4i:
(close  )
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Genetic Background:
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involves: C3H/HeH * C57BL/6
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Age:
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embryonic day 8.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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4-somite stage
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Specimen
4j:
embryonic day 8.0; Zic2iso/ Zic2iso
(more )
Note: 2-somite stage
Specimen
4j:
(close )
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Genetic Background:
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involves: C3H/HeH * C57BL/6
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Mutant Allele(s):
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Zic2iso/Zic2iso
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Age:
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embryonic day 8.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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2-somite stage
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Specimen
4k:
embryonic day 8.0; Zic2iso/ Zic2iso
(more )
Note: 4-somite stage
Specimen
4k:
(close )
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Genetic Background:
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involves: C3H/HeH * C57BL/6
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Mutant Allele(s):
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Zic2iso/Zic2iso
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Age:
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embryonic day 8.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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4-somite stage
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Specimen
4l:
embryonic day 8.0; Zic2iso/ Zic2iso
(more )
Note: 4-somite stage
Specimen
4l:
(close )
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Genetic Background:
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involves: C3H/HeH * C57BL/6
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Mutant Allele(s):
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Zic2iso/Zic2iso
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Age:
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embryonic day 8.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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4-somite stage
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Specimen
not shown WT/Het:
embryonic day 8.0
(more )
Note: 2-6 somites, summarized in Fig 4n
Specimen
not shown WT/Het:
(close )
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Genetic Background:
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involves: C3H/HeH * C57BL/6
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Age:
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embryonic day 8.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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2-6 somites, summarized in Fig 4n
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: lateral plate mesoderm |
Present |
Regionally restricted |
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Expressed in all wild-type embryos from 2 to 6 somites and was restricted to the left side. Strong expression in 13 out of 15 embryos. Weak expression in 2 embryos.
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Specimen
not shown WT/Het:
embryonic day 8.0
(more )
Note: 1-7 somites, summarized in Fig 4m
Specimen
not shown WT/Het:
(close )
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Genetic Background:
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involves: C3H/HeH * C57BL/6
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Age:
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embryonic day 8.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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1-7 somites, summarized in Fig 4m
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: node |
Present |
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Strong expression was enriched on the left side of the node (L>R) in 10 out of 18 embryos of 1-5 somites, enriched on the right side in one (L<R, 6 somites), and equally expressed (L=R) in 5 embryos of 1-4 somites. Weak expression enriched on the left side in two (L>R, one 6 somites, one 7 somites).
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Specimen
not shown iso/iso:
embryonic day 8.0; Zic2iso/ Zic2iso
(more )
Note: 2-4 somites, summarized in Fig 4m
Specimen
not shown iso/iso:
(close )
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Genetic Background:
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involves: C3H/HeH * C57BL/6
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Mutant Allele(s):
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Zic2iso/Zic2iso
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Age:
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embryonic day 8.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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2-4 somites, summarized in Fig 4m
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: node |
Weak |
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Weak expression was only detectable in early somite stages (2 to 4 somites) mutant embryos (4 out of 8): enriched on the left side (L>R) in 2 embryos, equally (L=R) in one, and enriched on the right side (L<R) in one.
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Specimen
not shown iso/iso:
embryonic day 8.0; Zic2iso/ Zic2iso
(more )
Note: 4-6 somites, summarized in Fig 4m
Specimen
not shown iso/iso:
(close )
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Genetic Background:
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involves: C3H/HeH * C57BL/6
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Mutant Allele(s):
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Zic2iso/Zic2iso
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Age:
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embryonic day 8.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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4-6 somites, summarized in Fig 4m
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: node |
Absent |
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No expression in 3 out of 8 embryos.
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Specimen
not shown iso/iso:
embryonic day 8.0; Zic2iso/ Zic2iso
(more )
Note: 1 somite, summarized in Fig 4m
Specimen
not shown iso/iso:
(close )
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Genetic Background:
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involves: C3H/HeH * C57BL/6
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Mutant Allele(s):
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Zic2iso/Zic2iso
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Age:
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embryonic day 8.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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1 somite, summarized in Fig 4m
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: node |
Strong |
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Bilaterally expressed (L=R) in 1 out of 8 embryos.
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Specimen
not shown iso/iso:
embryonic day 8.0; Zic2iso/ Zic2iso
(more )
Note: 1-7 somites, summarized in Fig 4n
Specimen
not shown iso/iso:
(close )
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Genetic Background:
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involves: C3H/HeH * C57BL/6
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Mutant Allele(s):
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Zic2iso/Zic2iso
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Age:
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embryonic day 8.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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1-7 somites, summarized in Fig 4n
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Specimen
not shown iso/iso:
embryonic day 8.0; Zic2iso/ Zic2iso
(more )
Note: 4 somites, summarized in Fig 4n
Specimen
not shown iso/iso:
(close )
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Genetic Background:
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involves: C3H/HeH * C57BL/6
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Mutant Allele(s):
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Zic2iso/Zic2iso
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Age:
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embryonic day 8.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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4 somites, summarized in Fig 4n
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: lateral plate mesoderm |
Weak |
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In two mutant embryos with expression, it was weak and either in the left or in the right side.
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