Probe:	Vps37a cDNA
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS13: embryo	Present	Ubiquitous	1A Vps37a
TS13: neural fold	Strong	Widespread	1A Vps37a	High enriched expression was detected in the neural folds along the entirety of the dorsal anterior-posterior axis.
TS13: 1st branchial arch	Strong	Regionally restricted	1A Vps37a	Expression was enriched at the distal tip.

Structure	Level	Pattern	Image	Note
TS15: embryo	Present	Ubiquitous	1B Vps37a
TS15: 1st branchial arch	Strong	Regionally restricted	1B Vps37a	Expression remained strongest at the rostral tip with reduced expression extending as far as its caudal boundary.
TS15: fronto-nasal process	Strong	Regionally restricted	1B Vps37a	High enrichment was present at the rostral tip with attenuated signal caudally; signal persisted along the dorsal anterior-posterior axis.
TS15: 2nd branchial arch	Strong		1B Vps37a
TS15: forelimb bud	Strong	Graded	1B Vps37a	Expression in a gradient with the highest level at the distal tip.

Structure	Level	Pattern	Image	Note
TS17: embryo	Present	Ubiquitous	2A Vps37a
TS17: forelimb bud	Present	Graded	2A Vps37a	Expression was highest in the apical ectodermal ridge and reduced towards the center of the bud.
TS17: forelimb bud apical ectodermal ridge	Present		2A Vps37a
TS17: hindlimb bud	Present	Regionally restricted	2A Vps37a	Expression in the emerging hindlimb is most apparent at the most distal tip with reduced expression proximally.

Structure	Level	Pattern	Image	Note
TS17: embryo	Present	Ubiquitous	2B Vps37a
TS17: latero-nasal process	Present	Regionally restricted	2B Vps37a	Expression was highest at the rim of the nasal pit perimeter.
TS17: medial-nasal process	Present	Regionally restricted	2B Vps37a	Expression was highest at the rim of the nasal pit perimeter.
TS17: 1st branchial arch maxillary component	Present	Regionally restricted	2B Vps37a	Expression was highest in the most posterior edge of the maxillary process.
TS17: 1st branchial arch mandibular component	Present	Regionally restricted	2B Vps37a	Expression was highest in the most anterior edge of the mandibular process.
TS17: 2nd branchial arch	Present	Regionally restricted	2B Vps37a	Expression was highest in the posterior edge with lower uniform expression seen throughout the rest of the arch.

Structure	Level	Pattern	Image	Note
TS19: embryo	Present	Ubiquitous	3A Vps37a
TS19: forelimb bud	Present	Regionally restricted	3A Vps37a	Expression appeared highest distally, with attenuated signal at the core of the limb buds.
TS19: hindlimb bud	Present	Regionally restricted	3A Vps37a	Expression appeared highest distally, with attenuated signal at the core of the limb buds.

Structure	Level	Pattern	Image	Note
TS19: embryo	Present	Ubiquitous	3B Vps37a
TS19: latero-nasal process	Present	Regionally restricted	3B Vps37a	Expression was highest in the lateral edges.
TS19: medial-nasal process	Present	Regionally restricted	3B Vps37a	Expression was highest in the posterior edge bordering the oral cavity.
TS19: 1st branchial arch maxillary component	Present	Regionally restricted	3B Vps37a	Expression was highest in the lateral edges.
TS19: mandibular process	Present	Regionally restricted	3B Vps37a	Signal in the mandibular process was highest in the medial anterior tips.
TS19: 2nd branchial arch	Weak		3B Vps37a