Probe:	Wnt16 probe
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS17: Rathke's pouch	Present	Regionally restricted	7f	Expression in Rathke' pouch beginning at E10.5. Expression concntrated dorsally. Less expression in area of differentiating cells of anterior lobe.
TS17: diencephalon	Present	Regionally restricted	7f	Expression in rostral lower domain of ventral diencephalon.
TS17: infundibular recess of 3rd ventricle	Present	Regionally restricted	7f	Expression excluded from infundibulum at all ages examined.

Structure	Level	Pattern	Image	Note
TS19: Rathke's pouch	Present	Regionally restricted	7g	Expression in Rathke' pouch. Expression concntrated dorsally. Less expression in area of differentiating cells of anterior lobe.
TS19: diencephalon	Present	Regionally restricted	7g	Expression in rostral lower domain of ventral diencephalon.
TS19: infundibular recess of 3rd ventricle	Absent		7g	Expression excluded from infundibulum at all ages examined.

Structure	Level	Pattern	Image	Note
TS20: remnant of Rathke's pouch	Present	Regionally restricted	7h	Expression in Rathke' pouch. Expression concntrated dorsally. Less expression in area of differentiating cells of anterior lobe.
TS20: diencephalon	Present	Regionally restricted	7h	Expression in rostral lower domain of ventral diencephalon to E12.5.
TS20: neurohypophysis infundibulum	Absent		7h	Expression excluded from infundibulum at all ages examined.

Structure	Level	Pattern	Image	Note
TS22: remnant of Rathke's pouch	Present	Regionally restricted	7i	Expression in Rathke' pouch. Expression concntrated dorsally. Less expression in area of differentiating cells of anterior lobe.
TS22: neurohypophysis infundibulum	Absent		7i	Expression excluded from infundibulum at all ages examined.

Structure	Level	Pattern	Image	Note
TS24: remnant of Rathke's pouch	Present		7j
TS24: neurohypophysis infundibulum	Absent		7j	Expression in Rathke' pouch. Expression concntrated dorsally. Less expression in area of differentiating cells of anterior lobe.