Antibody:	anti-Msi2
Detection system:	Alexa Fluor

Structure	Level	Pattern	Image	Note
TS17: telencephalon	Strong	Uniform	5A	Intense expression throughout telencephalon. Expression uniformly distributed in neuroepithelium. Co-expression with Msi1 in most cells.

Structure	Level	Pattern	Image	Note
TS17: telencephalon	Strong	Uniform	5C	Intense expression throughout telencephalon. Expression uniformly distributed in neuroepithelium. Co-expression with Mki67 in most cells.

Structure	Level	Pattern	Image	Note
TS17: telencephalon	Strong	Uniform	5E	Intense expression throughout telencephalon. Expression uniformly distributed in neuroepithelium. Co-expression with Nes in most cells.

Structure	Level	Pattern	Image	Note
TS20: telencephalon ventricular layer	Present		5G	Expression in ventricular cells of telencephalon. Co-expression with Msi1.

Structure	Level	Pattern	Image	Note
TS20: cerebral cortex ventricular layer	Present	Regionally restricted	5G inset	Cytoplasmic expression in neuroepithelial cells. Co-expression with Msi1.

Structure	Level	Pattern	Image	Note
TS22: ganglionic eminence	Present		5I	Expression co-localized with Msi1 in ventricular zone of telencephalon and ganglionic eminences. Co-expression with Msi1.
TS22: telencephalon ventricular layer	Present		5I	Expression co-localized with Msi1 in ventricular zone of telencephalon and ganglionic eminences. Co-expression with Msi1.

Structure	Cell Type	Level	Pattern	Image	Note
TS22: cerebral cortex intermediate zone	neuron	Absent		5K	No expression in Map2-expressing postimitotic neurons migrating in intermediate zone and cortical plate.
TS22: cortical plate	neuron	Absent		5K	No expression in Map2-expressing postimitotic neurons migrating in intermediate zone and cortical plate.

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex subventricular zone	Present		5M	Expression in many densely packed cells in subventricular zone bordered bt striatum and overlying the corpus callosum.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cerebral cortex subventricular zone		Present	Regionally restricted	5O	Expression in many densely packed cells in dorsolateral corner of subventricular zone.
TS28: cerebral cortex ventricular zone		Present		5O	Expression in many densely packed cells in ependymal layer.
TS28: cerebral cortex	astrocyte	Present	Scattered	5O	Expression in small scattered astrocytes in parenchyma.

Structure	Level	Pattern	Image	Note
TS17: future spinal cord	Strong	Uniform	6A	Intense expression throughout telencephalon. Expression uniformly distributed within neural tube. Co-expression with Msi1 in most cells.

Structure	Level	Pattern	Image	Note
TS17: future spinal cord	Present	Regionally restricted	6C	Expression in proliferating neuroepithelial cells. Co-expression with Msi1.

Structure	Level	Pattern	Image	Note
TS20: spinal cord	Present		6E	Co-expression with Msi1.

Structure	Cell Type	Level	Pattern	Image	Note
TS22: spinal cord ventricular layer		Present		6G	Expression in neuron-like cells in ventricular zone lining central canals.
TS22: spinal cord mantle layer	neuron	Present	Regionally restricted	6G	Expression in subset of migrating neurons sparcely distributed in ventrolateral area of mantle layer, co-expressed with Map2.

Structure	Level	Pattern	Image	Note
TS22: spinal cord	Present		6I	Co-expression with Msi1.

Structure	Level	Pattern	Image	Note
TS22: spinal cord ventricular layer	Present		6K	Expression in neuron-like cells in ventricular zone lining central canals. Co-expression with Msi1.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: spinal cord ependymal layer	ependymal cell	Strong		6M	Highly expressed in ependymal cells surrounding central canal.
TS28: spinal cord lumbar region	neuron	Present	Scattered	6M	Expression in some, but not all, populations of neurons scattered within parenchyma.

Structure	Level	Pattern	Image	Note
TS28: spinal cord lumbar region	Present	Regionally restricted	6O	Expression in neuron-like cells in adult spinal cord. No expression in some Hu-expressing neurons.
TS28: corpus striatum	Present		6O	Expression in neuron-like cells in striatum.

Structure	Level	Pattern	Image	Note
TS27: telencephalon subventricular zone	Present	Regionally restricted	7E	Expression in Gfap-negative densely packed subventricular zone cells.

Structure	Cell Type	Level	Pattern	Image	Note
TS27: telencephalon subventricular zone	neuron	Present	Regionally restricted	7F	Expression in cells intermingled with, but not co-expressed with Hu-positive immature neurons within dorsomateral area of subventricular zone.

Structure	Cell Type	Level	Pattern	Image	Note
TS27: telencephalon subventricular zone	neuron	Present	Regionally restricted		No expression in Tubb3-expressing immature neurons and their tangled neurites in subventricular zone.

Structure	Cell Type	Level	Pattern	Image	Note
TS27: telencephalon subventricular zone	oligodendrocyte precursor cell	Absent		7G	No expression in Pdgfra-positive oligodendrocyte precursor cells.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: neocortex	astrocyte	Present	Widespread	8A	Expression in many astrocytes with small cell bodies throughtout the cortex.
TS28: cortical layer II	neuron	Present		8A	Expression in subset of neurons with larger cell bodies found sparcely in layers II-III of neocortex.
TS28: cortical layer III	neuron	Present		8A	Expression in subset of neurons with larger cell bodies found sparcely in layers II-III of neocortex.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: neocortex	astrocyte	Present	Widespread	8C	Expression in many astrocytes with small cell bodies throughtout the cortex.
TS28: cortical layer II	neuron	Present		8C	Expression in subset of neurons with larger cell bodies found sparcely in layers II-III.
TS28: cortical layer III	neuron	Present		8C	Expression in subset of neurons with larger cell bodies found sparcely in layers II-III.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cortical layer II	astrocyte	Present		8E	Expression in gray matter (layers II-III). Some of these cells co-expressed Msi1 and had relatively thick and branched processes, characteristic of astrocytes.
TS28: cortical layer III	astrocyte	Present		8E	Expression in gray matter (layers II-III). Some of these cells co-expressed Msi1 and had relatively thick and branched processes, characteristic of astrocytes.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cortical layer II	neuron	Present		8G	Co-expressed with Hu in a few round neuronal cells. but lack of expression in majority of Hu-positive neurons.
TS28: cortical layer III	neuron	Present		8G	Co-expressed with Hu in a few round neuronal cells. but lack of expression in majority of Hu-positive neurons.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cortical layer I		Present		8I	Expression in white matter, predominantly distributed in molecular layer (layer I).
TS28: neocortex	astrocyte	Present	Regionally restricted	8I	Expression co-localized with Gfap in fibrous astrocytes near pial suface.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: neocortex	oligodendrocyte precursor cell	Present	Regionally restricted	8K	Expression in subcortical white matter. No expression in Cnp-positive oligodendroglial cell bodies predominant in subcortical white matter.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cortical layer II	neuron	Present		9A	Co-expression with Pvalb in GABAergic nonpyramidal neurons in neocortex layers II-III.
TS28: cortical layer III	neuron	Present		9A	Co-expression with Pvalb in GABAergic nonpyramidal neurons in neocortex layers II-III.
TS28: neocortex	astrocyte	Present		9A	Faint expression in fine-branched processes and small cell bodies of astrocytes.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cortical layer V	neuron	Present		9C	Co-expression with Pvalb in neurons in deeper layer V.
TS28: neocortex	Betz cell	Absent		9C	No expression in somata of large pyramidal neurons (presumptive Betz cells) surrounded by Pvalb-positive multi-terminal endings.

Structure	Level	Pattern	Image	Note
TS28: cortical layer II	Present	Regionally restricted	9E	Expression in neurons in neocortex layers II-III, intermingled but not ovelapping with Calb1-expressing neurons.
TS28: cortical layer III	Present	Regionally restricted	9E	Expression in neurons in neocortex layers II-III, intermingled but not ovelapping with Calb1-expressing neurons.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cortical layer II	neuron	Present	Regionally restricted	9G	Expression in neurons in layers II-III. No co-expression Calb1.
TS28: cortical layer III	neuron	Present	Regionally restricted	9G	Expression in neurons in layers II-III. No co-expression Calb1.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cortical layer II	GABAergic neuron	Present		9I	Expression in layers II-III in GABA-containing neurons.
TS28: cortical layer III	GABAergic neuron	Present		9I	Expression in layers II-III in GABA-containing neurons.

Structure	Level	Pattern	Image	Note
TS28: neocortex	Present	Regionally restricted	9K	No expression in some GABA-containing neurons.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: neocortex	cholinergic neuron	Absent		9M	No expression in Chat-positive cholinergic neurons in gray matter of neocortex.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: hippocampus CA1	interneuron	Strong		9O	Intense expression in small number of neurons. Co-expression with Pvalb in interneurons in CA1 subfield.
TS28: hippocampus	astrocyte	Present	Diffuse	9O	Diffuse expression in astrocytes uniformly distributed within this region.
TS28: CA1 pyramidal cell layer	pyramidal neuron	Absent		9O	No expression in numerous CA1 pyramidal neurons aligned in rows.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: nucleus of diagonal band	cholinergic neuron	Present		9Q	Exression in several Chat-positive cholinergic projection neurons in nuclei of diagonal bands (Ch2 and Ch3).

Structure	Cell Type	Level	Pattern	Image	Note
TS28: zona incerta	dopaminergic neuron	Present		9S	Expression in Th-positive dopaminergic neurons (A13) within zona incerta of subthalamic region.

Structure	Level	Pattern	Image	Note
TS22: 4th ventricle lateral recess	Present	Regionally restricted		Expression in small tightly packed cells of external granule cell layer migrating out of lateral recess.
TS22: 4th ventricle ependyma	Present			Expression in ventricular zone of fourth ventricle.

Structure	Cell Type	Level	Pattern	Image	Note
TS27: cerebellum Purkinje cell layer	Purkinje cell	Strong		10B	Prominent expression in large Purkinje neurons in Purkinje cell layer.
TS27: cerebellum external granule cell layer		Weak		10B	Faint expression in external granule cell layer.
TS27: cerebellum white matter	glial restricted tripotential precursor cell	Weak		10B	Faint expression in glial precursor cells in white matter
TS27: cerebellum	astrocyte	Present		10B	Expression in astrocytes in central white matter tracts.
TS27: deep cerebellar nucleus	neuron	Present		10B	Expression in differentiated neurons in deep cerebellar nuclei.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Purkinje cell	Strong		10D	Prominent expression in large Purkinje neurons in Purkinje cell layer.
TS28: cerebellum	Bergmann glial cell	Present		10D	Expression in Bergmann glial cells with cell bodies adjacent to cell bodies of Purkinje cells and extending tangential processes into molecular layer.
TS28: cerebellum molecular layer	cerebellar stellate cell	Present		10D	Expression in interneurons such as stellate cells and basket cells in molecular layer.
TS28: cerebellum molecular layer	cerebellum basket cell	Present		10D	Expression in interneurons such as stellate cells and basket cells in molecular layer.
TS28: cerebellum internal granule cell layer	cerebellar Golgi cell	Present		10D	Exression in Golgi neuron in internal granule cell layer.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cerebellum external granule cell layer		Weak		10F	Faint expression in external granule cell layer.
TS28: cerebellum Purkinje cell layer		Present		10F	Expression in Purkinje cells in Purkinje cell layer forming a single row.
TS28: cerebellum	Bergmann glial cell	Present	Regionally restricted	10F	Expression in many small cells in vicinity of Purkinje cells - likely to be developing Bergmann glia.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cerebellum external granule cell layer		Weak		10H	Low level expression in external granule cell layer.
TS28: cerebellum Purkinje cell layer	Purkinje cell	Present		10H	Expression in Purkinje cells in Purkinje cell layer forming a single row.
TS28: cerebellum	Bergmann glial cell	Present		10H	Expression in many small cells in vicinity of Purkinje cells - likely to be developing Bergmann glia.
TS28: cerebellum internal granule cell layer	cerebellar granule cell	Absent		10H	No expression in Hu-positive granule neurons in internal granule cell layer.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Purkinje cell	Present		10J	Expression in Purkinje cells in Purkinje cell layer forming a single row.
TS28: cerebellum	Bergmann glial cell	Present	Regionally restricted	10J	Expression in soma of Bergman glial cells and their fibers coursing from Purkinje cell layer through molecular layer to pial surface.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: dentate gyrus subgranular zone		Present			Expression in occasional cells in subgranular region.
TS28: dentate gyrus	dentate gyrus granule cell	Present	Regionally restricted		No expression in many granule neurons of dentate gyrus.
TS28: caudate-putamen	neuron	Present	Regionally restricted		Expression in subpopulations of neurons sparsely distributed in ventral pallidum, globus pallidus and caudate putamen.
TS28: globus pallidus	neuron	Present	Regionally restricted		Expression in subpopulations of neurons sparsely distributed in ventral pallidum, globus pallidus and caudate putamen.
TS28: corpus striatum	cholinergic neuron	Present			Expression in cholinergic projection neurons in striatum, medial septal nucleus (Ch1) and medial habenular nucleus (Ch7).
TS28: medial habenular nucleus	cholinergic neuron	Present			Expression in cholinergic projection neurons in striatum, medial septal nucleus (Ch1) and medial habenular nucleus (Ch7).
TS28: medial septal nucleus	cholinergic neuron	Present			Expression in cholinergic projection neurons in striatum, medial septal nucleus (Ch1) and medial habenular nucleus (Ch7).
TS28: lateral habenular nucleus	neuron	Present			Neuronal expression in medial preoptic nucleus, supraoptic nucleus, periventricular nucleus and lateral habenular nucleus.
TS28: medial preoptic nucleus	neuron	Present			Neuronal expression in medial preoptic nucleus, supraoptic nucleus, periventricular nucleus and lateral habenular nucleus.
TS28: supraoptic nucleus	neuron	Present			Neuronal expression in medial preoptic nucleus, supraoptic nucleus, periventricular nucleus and lateral habenular nucleus.