Probe:	png-1 cDNA
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS23: cerebellum	Present		4A
TS23: cerebral cortex	Present		4A
TS23: dorsal root ganglion	Present		4A
TS23: hindbrain	Present		4A
TS23: medulla oblongata	Present		4A
TS23: midbrain	Present		4A
TS23: olfactory epithelium	Present		4A
TS23: pons	Present		4A
TS23: spinal cord	Present		4A
TS23: thalamus	Present		4A
TS23: trigeminal ganglion	Present		4A
TS23: brain	Present	Regionally restricted	4A	Expression is in postmitotic regions and is absent in proliferative zones abutting the ventricles.
TS23: embryo	Present	Regionally restricted	4A	Expression is in throughout the central nervous system and in developing peripheral neural structures and is absent in all non-neural tissues examined.

Structure	Level	Pattern	Image	Note
TS17: telencephalon	Absent		5A

Structure	Level	Pattern	Image	Note
TS19: telencephalon	Present	Regionally restricted	5C	Expression is in a thin band of cells, one to three cells thick, near the pial surface and was absent from the rest of the telencephalon.

Structure	Level	Pattern	Image	Note
TS21: cortical preplate	Present		5E
TS21: cerebral cortex ventricular layer	Present	Single cells	5E	Expression was virtually absent from the proliferative ventricular zone.

Structure	Level	Pattern	Image	Note
TS23: cerebral cortex intermediate zone	Present		5G
TS23: cerebral cortex marginal layer	Present		5G
TS23: cortical plate	Present		5G
TS23: cortical subplate	Present		5G
TS23: cerebral cortex ventricular layer	Present	Single cells	5G	Expression was virtually absent from the proliferative ventricular zone.

Structure	Level	Pattern	Image	Note
TS25: cerebral cortex intermediate zone	Present		5I
TS25: cerebral cortex marginal zone	Present		5I
TS25: cortical plate	Present		5I
TS25: cortical subplate	Present		5I
TS25: cerebral cortex ventricular layer	Present	Single cells	5I	Expression was virtually absent from the proliferative ventricular zone.

Structure	Level	Pattern	Image	Note
TS28: cortical layer II	Present		5K	Expression is in scattered large cells throughout the layer.
TS28: cortical layer III	Present		5K	Expression is in scattered large cells throughout the layer.
TS28: cortical layer IV	Present		5K	Expression is in scattered large cells throughout the layer.
TS28: cortical layer V	Present		5K	Expression is in scattered large cells throughout the layer.
TS28: cortical layer VI	Present		5K	Expression is in scattered large cells throughout the layer.
TS28: cortical layer I	Present	Single cells	5K	Very little expression was detected.

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex ventricular layer	Present	Single cells	6A , 6B	Expression is virtually absent and the rare positive cells are not BrdU positive.
TS22: cerebral cortex intermediate zone	Present		6C , 6A	Expression is throughout and is absent in the very few BrdU-positive cells.

Structure	Level	Pattern	Image	Note
TS23: retina layer	Present	Regionally restricted	7A	Expression is in the postmitotic ganglion cell layer and is absent in the proliferative neuroblast layer.

Structure	Level	Pattern	Image	Note
TS23: olfactory epithelium	Present	Regionally restricted	7C	Expression is in regions containing differentiating receptor neurons and is absent in the inner (apical) zones of proliferating stem cells and epithelial supporting cells.

Structure	Level	Pattern	Image	Note
TS20: telencephalon	Present	Graded	8A	Expression is in a band of cells four to six cells thick in the ventrolateral cortex that tapers gradually in the dorsomedial direction to a single cell thickness.
TS20: ganglionic eminence	Present	Regionally restricted	8A	Expression is in the postmitotic zone.

Structure	Level	Pattern	Image	Note
TS20: spinal cord	Present	Graded	8B	Expression is most extensive ventrally and tapers in more dorsal regions.
TS20: dorsal root ganglion	Present		8B