Antibody:	clone 3B5
Detection system:	Secondary antibody coupled to Alexa Fluor 555

Structure	Level	Pattern	Image	Note
TS20: cerebellum primordium	Present	Regionally restricted	3D , 3F	Coexpressed with Tfap2b in the ventricular zone. Some Tfap2b+ cells did not express Tfap2a.
TS20: cerebellum white matter	Present		3F , 3D	Coexpressed with Tfap2b.

Structure	Cell Type	Level	Pattern	Image	Note
TS20: cerebellum primordium	cerebellar inhibitory GABAergic interneuron	Present		3G , 3J	Coexpressed with Olig2 and Lhx1/5 (early postmitotic GABAergic neurons) in the ventricular zone. Not all Olig2-expressing cells in the ventricular zone expressed Tfap2a.
TS20: cerebellum white matter	cerebellar inhibitory GABAergic interneuron	Present		3J , 3G	Only coexpressed Lhx1/5 (postmitotic GABAergic neurons).

Structure	Cell Type	Level	Pattern	Image	Note
TS23: cerebellum layer	Purkinje cell	Present	Regionally restricted	4C' , 4B' , 4A	Coexpressed with Rora under the external germinal layer.
TS23: cerebellum	cerebellar inhibitory GABAergic interneuron	Present	Regionally restricted	4B' , 4A , 4C'	Coexpressed with Pax2 in the ventral region.
TS23: cerebellum white matter		Present		4B' , 4A , 4C'	There were also positive cells that did not coexpressed with Rora or Pax2.

Structure	Cell Type	Level	Pattern	Image	Note
TS26: cerebellum Purkinje cell layer	Purkinje cell	Present		4H' , 4I' , 4G	Coexpressed with Rora.
TS26: cerebellum white matter	cerebellar inhibitory GABAergic interneuron	Present		4G , 4H' , 4I'	Coexpressed with Pax2, increased 5% compared with that of E15.5. The Tfap2a+ only cells increased 3%, although not statistically significant.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Purkinje cell	Present	Regionally restricted	S1C' , S1D' , S1B'" , S1C'" , S1D"' , S1B' , S1A	Expressed in a subset of cells.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Purkinje cell	Present	Uniform	S1H' , S1G' , S1F"' , S1H"' , S1F' , S1G"' , S1E	Uniformly expressed in parvalbumin+ Purkinje cells.

Structure	Level	Pattern	Image	Note
TS20: cerebellum white matter	Present		S3D , S3F	Coexpressed with Pax2 in GABAergic interneuron precursors.
TS20: cerebellum primordium	Present	Regionally restricted	S3F , S3D	Expressed in the ventricular zone. Coexpressed with Pax2 in GABAergic interneuron precursors.