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Gene Expression Data
Assay Details
Assay
Reference: J:338453 Kim MK, et al., Spatiotemporal and functional characterisation of transient receptor potential vanilloid 4 (TRPV4) in the murine intervertebral disc. Eur Cell Mater. 2021 Feb 23;41:194-203
Assay type: In situ reporter (knock in)
MGI Accession ID: MGI:7516513
Gene symbol: Trpv4
Gene name: transient receptor potential cation channel, subfamily V, member 4
Reporter: lacZ
Antibody: anti-B-galactosidase
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen 2b E12.5: embryonic day 12.5; Trpv4tm1b(KOMP)Wtsi/Trpv4+ (more )
Structure Level Pattern Image Note
TS20: notochord Present 2b brightfield , 2b beta-galactosidase Expression localized to notochord.
TS20: axial mesoderm Present Regionally restricted 2b beta-galactosidase , 2b brightfield Expression in intervertebral mesenchyme.
TS20: vertebral pre-cartilage condensation Absent 2b beta-galactosidase , 2b brightfield No expression in pre-vertebra region of developing spine.
Specimen 2c E17.5: embryonic day 17.5; Trpv4tm1b(KOMP)Wtsi/Trpv4+ (more )
Structure Level Pattern Image Note
TS25: annulus fibrosus Present 2c beta-galactosidase , 2c brightfield Expression in primitive annulus fibrosus, nucleus pulposus and pre-vertebra.
TS25: nucleus pulposus Present 2c beta-galactosidase , 2c brightfield Expression in primitive annulus fibrosus, nucleus pulposus and pre-vertebra.
Specimen 2d PN1: postnatal day 1; Trpv4tm1b(KOMP)Wtsi/Trpv4+ (more )
Structure Level Pattern Image Note
TS27: annulus fibrosus Present 2d brightfield , 2d beta-galactosidase Expression in primitive annulus fibrosus, nucleus pulposus and pre-vertebra.
TS27: nucleus pulposus Present 2d brightfield , 2d beta-galactosidase Expression in primitive annulus fibrosus, nucleus pulposus and pre-vertebra.
Specimen 3a: postnatal month 2.5; Trpv4tm1b(KOMP)Wtsi/Trpv4+ (more )
Structure Level Pattern Image Note
TS28: intervertebral disc Present Regionally restricted 3a Beta-galactosidase , 3a Brightfield , 3a Beta-galactosidase DAPI Expression in lumbar intervertebral disc.
TS28: nucleus pulposus Present 3a Brightfield , 3a Beta-galactosidase DAPI , 3a Beta-galactosidase
TS28: annulus fibrosus Present Regionally restricted 3a Beta-galactosidase , 3a Beta-galactosidase DAPI , 3a Brightfield Expression in inner annulus fibrosus cells. No expression in outer annulus fibrosus.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory