Gene Expression Data
Assay Details
Assay
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Reference:
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J:109419
Wadehra M, et al., Knockdown of the tetraspan protein epithelial membrane protein-2 inhibits implantation in the mouse. Dev Biol. 2006 Apr 15;292(2):430-41
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7516309
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Gene symbol:
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Emp2
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Gene name:
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epithelial membrane protein 2
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Antibody:
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Anti-Emp2
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
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Results
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Specimen
1A:
postnatal adult
(more )
Note: Fix was either 4%paraformaldehyde or 10% formalin. Pregnant uterus - day 1 pc.
Specimen
1A:
(close )
Genetic Background:
|
CF-1
|
Age:
|
postnatal adult
|
Sex:
|
Female
|
Type:
|
section
|
Fixation:
|
Other - See Notes
|
Embedding:
|
Paraffin
|
Note:
|
Fix was either 4%paraformaldehyde or 10% formalin. Pregnant uterus - day 1 pc.
|
Specimen
1B:
postnatal adult
(more )
Note: Fix was either 4%paraformaldehyde or 10% formalin. Pregnant uterus - day 1 pc.
Specimen
1B:
(close )
Genetic Background:
|
CF-1
|
Age:
|
postnatal adult
|
Sex:
|
Female
|
Type:
|
section
|
Fixation:
|
Other - See Notes
|
Embedding:
|
Paraffin
|
Note:
|
Fix was either 4%paraformaldehyde or 10% formalin. Pregnant uterus - day 1 pc.
|
Specimen
1C:
postnatal adult
(more )
Note: Fix was either 4%paraformaldehyde or 10% formalin. Pregnant uterus - day 1 pc.
Specimen
1C:
(close )
Genetic Background:
|
CF-1
|
Age:
|
postnatal adult
|
Sex:
|
Female
|
Type:
|
section
|
Fixation:
|
Other - See Notes
|
Embedding:
|
Paraffin
|
Note:
|
Fix was either 4%paraformaldehyde or 10% formalin. Pregnant uterus - day 1 pc.
|
Specimen
1D:
postnatal adult
(more )
Note: Fix was either 4%paraformaldehyde or 10% formalin. Pregnant uterus - day 4 pc.
Specimen
1D:
(close )
Genetic Background:
|
CF-1
|
Age:
|
postnatal adult
|
Sex:
|
Female
|
Type:
|
section
|
Fixation:
|
Other - See Notes
|
Embedding:
|
Paraffin
|
Note:
|
Fix was either 4%paraformaldehyde or 10% formalin. Pregnant uterus - day 4 pc.
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: endometrium glandular epithelium |
Present |
Regionally restricted |
1D
|
Expression was in the supranuclear region of the intracellular compartment, but staining was more diffuse. Staining was localized to the apical cell surface.
|
TS28: endometrium luminal epithelium |
Present |
Regionally restricted |
1D
|
Expression was in the supranuclear region of the intracellular compartment, but staining was more diffuse. Staining was localized to the apical cell surface.
|
TS28: endometrium stroma |
Present |
|
1D
|
|
Specimen
1E:
postnatal adult
(more )
Note: Fix was either 4%paraformaldehyde or 10% formalin. Pregnant uterus - day 4 pc.
Specimen
1E:
(close )
Genetic Background:
|
CF-1
|
Age:
|
postnatal adult
|
Sex:
|
Female
|
Type:
|
section
|
Fixation:
|
Other - See Notes
|
Embedding:
|
Paraffin
|
Note:
|
Fix was either 4%paraformaldehyde or 10% formalin. Pregnant uterus - day 4 pc.
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: endometrium glandular epithelium |
Present |
Regionally restricted |
1E
|
Expression was in the supranuclear region of the intracellular compartment, but staining was more diffuse. Staining was localized to the apical cell surface.
|
TS28: endometrium luminal epithelium |
Present |
Regionally restricted |
1E
|
Expression was in the supranuclear region of the intracellular compartment, but staining was more diffuse. Staining was localized to the apical cell surface.
|
TS28: endometrium stroma |
Present |
|
1E
|
|
Specimen
1F:
postnatal adult
(more )
Note: Fix was either 4%paraformaldehyde or 10% formalin. Pregnant uterus - day 4 pc.
Specimen
1F:
(close )
Genetic Background:
|
CF-1
|
Age:
|
postnatal adult
|
Sex:
|
Female
|
Type:
|
section
|
Fixation:
|
Other - See Notes
|
Embedding:
|
Paraffin
|
Note:
|
Fix was either 4%paraformaldehyde or 10% formalin. Pregnant uterus - day 4 pc.
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: endometrium glandular epithelium |
Present |
Regionally restricted |
1F
|
Expression was in the supranuclear region of the intracellular compartment, but staining was more diffuse. Staining was localized to the apical cell surface.
|
TS28: endometrium luminal epithelium |
Present |
Regionally restricted |
1F
|
Expression was in the supranuclear region of the intracellular compartment, but staining was more diffuse. Staining was localized to the apical cell surface.
|
TS28: endometrium stroma |
Present |
|
1F
|
|
Specimen
2C maternal:
postnatal adult
(more )
Note: Fix was either 4%paraformaldehyde or 10% formalin. Pregnant uterus - day 6 pc.
Specimen
2C maternal:
(close )
Genetic Background:
|
CF-1
|
Age:
|
postnatal adult
|
Sex:
|
Female
|
Type:
|
section
|
Fixation:
|
Other - See Notes
|
Embedding:
|
Paraffin
|
Note:
|
Fix was either 4%paraformaldehyde or 10% formalin. Pregnant uterus - day 6 pc.
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS28: decidua |
decidual cell |
Present |
Regionally restricted |
2C
|
Decidualized stromal cells at the site of implantation expressed relatively high levels of Emp2.
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Specimen
2C embryo:
embryonic day 5.5
(more )
Note: Fix was either 4%paraformaldehyde or 10% formalin.
Specimen
2C embryo:
(close )
Genetic Background:
|
CF-1
|
Age:
|
embryonic day 5.5
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
Sex:
|
Female
|
Type:
|
section
|
Fixation:
|
Other - See Notes
|
Embedding:
|
Paraffin
|
Note:
|
Fix was either 4%paraformaldehyde or 10% formalin.
|
Specimen
2D maternal:
postnatal adult
(more )
Note: Fix was either 4%paraformaldehyde or 10% formalin. Pregnant uterus - day 6 pc.
Specimen
2D maternal:
(close )
Genetic Background:
|
CF-1
|
Age:
|
postnatal adult
|
Sex:
|
Female
|
Type:
|
section
|
Fixation:
|
Other - See Notes
|
Embedding:
|
Paraffin
|
Note:
|
Fix was either 4%paraformaldehyde or 10% formalin. Pregnant uterus - day 6 pc.
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS28: decidua |
decidual cell |
Present |
Regionally restricted |
2D
|
Decidualized stromal cells at the site of implantation expressed relatively high levels of Emp2.
|
Specimen
2D embryo:
embryonic day 5.5
(more )
Note: Fix was either 4%paraformaldehyde or 10% formalin.
Specimen
2D embryo:
(close )
Genetic Background:
|
CF-1
|
Age:
|
embryonic day 5.5
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
Sex:
|
Female
|
Type:
|
section
|
Fixation:
|
Other - See Notes
|
Embedding:
|
Paraffin
|
Note:
|
Fix was either 4%paraformaldehyde or 10% formalin.
|
Specimen
2E:
postnatal adult
(more )
Note: Fix was either 4%paraformaldehyde or 10% formalin. Pregnant uterus - day 7 pc.
Specimen
2E:
(close )
Genetic Background:
|
CF-1
|
Age:
|
postnatal adult
|
Sex:
|
Female
|
Type:
|
section
|
Fixation:
|
Other - See Notes
|
Embedding:
|
Paraffin
|
Note:
|
Fix was either 4%paraformaldehyde or 10% formalin. Pregnant uterus - day 7 pc.
|
Specimen
2F:
postnatal adult
(more )
Note: Fix was either 4%paraformaldehyde or 10% formalin. Pregnant uterus - day 7 pc.
Specimen
2F:
(close )
Genetic Background:
|
CF-1
|
Age:
|
postnatal adult
|
Sex:
|
Female
|
Type:
|
section
|
Fixation:
|
Other - See Notes
|
Embedding:
|
Paraffin
|
Note:
|
Fix was either 4%paraformaldehyde or 10% formalin. Pregnant uterus - day 7 pc.
|
|