Summary | Mutation origin | Mutation description | Recombinase activity | Find Mice (IMSR) | References

Symbol:	Myh11em1(icre)Jifz
Name:	myosin, heavy polypeptide 11, smooth muscle; endonuclease-mediated mutation 1, Jifeng Zhang
MGI ID:	MGI:7512809
Synonyms:	Myh11-CreNLSP2A, Myh11em1(cre)Jifz
Gene:	Myh11  Location: Chr16:14012392-14109227 bp, - strand  Genetic Position: Chr16, 9.71 cM
Alliance:	Myh11em1(icre)Jifz page

Strain of Origin:

C57BL/6J x SJL

Allele Type:    Endonuclease-mediated (Recombinase) Mutation:    Insertion   Myh11em1(icre)Jifz expression driven by 1 gene Knock-in expression driven by: Organism Driver Gene Note mouse Myh11 (MGI:102643)   Mutation details: Using CRISPR/Cas9 technology, a a nuclear localization sequence (NLS) followed by a codon optimized Cre recombinase fused to ERT2, a GSG linker and a P2A peptide (for production of a bicistronic mRNA transcript) immediately was inserted into the endogenous Myh11 gene immediately after the first codon ATG in exon 2. Cre expression is constitutive rather than inducible by tamoxifen. (J:338442)

Activity:	Tissue activity of this recombinase allele
Driver:	Myh11 (mouse) Summary of all recombinase alleles driven by Myh11. Alliance mouse Myh11 gene page

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Myh11 Mutation:	93 strains or lines available

Original:	J:338442 Zhao Y, et al., Generating endogenous Myh11-driven Cre mice for sex-independent gene deletion in smooth muscle cells. JCI Insight. 2023 Jul 24;8(14):e171661
All:	1 reference(s)