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Gene Expression Data
Assay Details
Assay
Reference: J:191149 Alves CH, et al., Loss of CRB2 in the mouse retina mimics human retinitis pigmentosa due to mutations in the CRB1 gene. Hum Mol Genet. 2013 Jan 1;22(1):35-50
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7506986
Gene symbol: Rcvrn
Gene name: recoverin
Antibody: Anti-Recoverin (AB5585)
Detection system: Secondary antibody coupled to Cy3
Results
Specimen 7A: postnatal day 3; Crb2tm1.1Wij/? (more )
Note: Controls could be Crb2F/F or Crb2F/+/Chx10Cre+/-.
Structure Cell Type Level Pattern Image Note
TS27: photoreceptor layer photoreceptor cell Present Regionally restricted 7A Exression was in immature as well as mature photoreceptors.
TS27: retina inner nuclear layer cone retinal bipolar cell Present Regionally restricted 7A Expression was in some cone bipolar cells.
Specimen 7B: postnatal day 3; Crb2tm1.1Wij/Crb2tm1.1Wij, Tg(Chx10-EGFP/cre,-ALPP)2Clc/0 (more )
Note: Conditional mutant.
Structure Cell Type Level Pattern Image Note
TS27: photoreceptor layer photoreceptor cell Present 7B The number of immunopositive photoreceptors was higher in the cKO compared with control.
TS27: retina inner nuclear layer cone retinal bipolar cell Present 7B
TS27: neural retina Present Regionally restricted 7B In regions where the adherens junctions were disrupted in the mutant, Recoverin-positive cells were displaced from the apical surface and localized ectopically in the center of the neuroepithelial layer.
Specimen 7C: postnatal day 6; Crb2tm1.1Wij/? (more )
Note: Controls could be Crb2F/F or Crb2F/+/Chx10Cre+/-. Double labeled: red - Rcvrn; green - Rho (assay MGI:7506988).
Structure Cell Type Level Pattern Image Note
TS28: photoreceptor layer photoreceptor cell Present 7C Exression was in immature as well as mature photoreceptors.
TS28: retina inner nuclear layer cone retinal bipolar cell Present Regionally restricted 7C Expression was in some cone bipolar cells.
Specimen 7D: postnatal day 6; Crb2tm1.1Wij/Crb2tm1.1Wij, Tg(Chx10-EGFP/cre,-ALPP)2Clc/0 (more )
Note: Conditional mutant. Double labeled: red - Rcvrn; green - Rho (assay MGI:7506988).
Structure Level Pattern Image Note
TS28: intraretinal space Present Regionally restricted 7D Some ectopic nuclei were detected in the subretinal space throughout the retina situated alone or in small clusters. Some of these nuclei were positive for Recoverin, but negative for Rhodopsin, while others were positive for both.
Specimen 7E: postnatal day 10; Crb2tm1.1Wij/? (more )
Note: Controls could be Crb2F/F or Crb2F/+/Chx10Cre+/-.
Structure Cell Type Level Pattern Image Note
TS28: photoreceptor layer photoreceptor cell Present 7E
Specimen 7F: postnatal day 10; Crb2tm1.1Wij/Crb2tm1.1Wij, Tg(Chx10-EGFP/cre,-ALPP)2Clc/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS28: intraretinal space Present Regionally restricted 7F Most of the nuclei localized in the subretinal space were immunopositive.
Specimen 7I: postnatal month 3; Crb2tm1.1Wij/? (more )
Note: Controls could be Crb2F/F or Crb2F/+/Chx10Cre+/-. Double labeled: red - Rcvrn; green - Rho (assay MGI:7506988).
Structure Cell Type Level Pattern Image Note
TS28: photoreceptor layer photoreceptor cell Present 7I
Specimen 7J: postnatal month 3; Crb2tm1.1Wij/Crb2tm1.1Wij, Tg(Chx10-EGFP/cre,-ALPP)2Clc/0 (more )
Note: Conditional mutant. Double labeled: red - Rcvrn; green - Rho (assay MGI:7506988).
Structure Cell Type Level Pattern Image Note
TS28: photoreceptor layer photoreceptor cell Present Regionally restricted 7J The photoreceptor layer was reduced to few nuclei in a row.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/23/2024
MGI 6.23 		The Jackson Laboratory