Gene Expression Data
Assay Details
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Assay
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Reference:
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J:200623
Giesert F, et al., Expression analysis of Lrrk1, Lrrk2 and Lrrk2 splice variants in mice. PLoS One. 2013;8(5):e63778
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:7506541
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Gene symbol:
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Lrrk2
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Gene name:
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leucine-rich repeat kinase 2
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Probe:
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Lrrk2 cDNA
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Results
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Specimen
5B:
postnatal
(more  )
Note: Double labeled: black - Lrrk2; green - Drd1 (assay MGI:7506546).
Specimen
5B:
(close  )
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Genetic Background:
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C57BL/6J
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Age:
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postnatal
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Double labeled: black - Lrrk2; green - Drd1 (assay MGI:7506546).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: putamen |
Present |
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5B overlay
,
5B Lrrk2
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There were 36% Lrrk2-positive, 25% Drd1-positive and 39% double positive cells.
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Specimen
5C:
postnatal
(more )
Note: Double labeled: black - Lrrk2; green - Drd2 (assay MGI:7506561).
Specimen
5C:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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postnatal
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Double labeled: black - Lrrk2; green - Drd2 (assay MGI:7506561).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: putamen |
Present |
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5C overlay
,
5C Lrrk2
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There were 38% Lrrk2-positive, 24% Drd2-positive and 37% double positive cells.
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Specimen
not shown NeuN:
postnatal
(more )
Note: Also stained for Rbfox3 (assay MGI:7507055).
Specimen
not shown NeuN:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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postnatal
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Also stained for Rbfox3 (assay MGI:7507055).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: corpus striatum |
Present |
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There was a high overlap of Lrrk2 mRNA expressing cells with Rbfox3.
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Specimen
not shown DARPP-32:
postnatal
(more )
Note: Also stained for Ppp1r1b (assay MGI:7507052).
Specimen
not shown DARPP-32:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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postnatal
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Also stained for Ppp1r1b (assay MGI:7507052).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: corpus striatum |
Present |
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Striatal cells strongly expressing Lrrk2 were also positive for Ppp1r1b.
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Analysis Tools