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Gene Expression Data
Assay Details
Assay
Reference: J:320624 Tan BSN, et al., Regulation of amino acid transporters in pluripotent cell populations in the embryo and in culture; novel roles for sodium-coupled neutral amino acid transporters. Mech Dev. 2016 Aug;141:32-39
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7506184
Gene symbol: Slc38a1
Gene name: solute carrier family 38, member 1
Antibody: anti-SNAT1 (sc-67080)
Detection system: Secondary antibody coupled to Alexa Fluor 568
Results
Specimen 1A 2-cell: embryonic day 1.5 (more )
Note: 2-cell embryo. Double labeled: red - Slc38a1; green - Pou5f1 (assay MGI:7506229).
Structure Level Pattern Image Note
TS2: 2-cell stage embryo Present 1A 2-cell SNAT1 , 1A 2-cell merge Expression is cytoplasmic and nuclear.
Specimen 1A 4-cell: embryonic day 2.0 (more )
Note: 4-cell embryo. Double labeled: red - Slc38a1; green - Pou5f1 (assay MGI:7506229).
Structure Level Pattern Image Note
TS3: 4-cell stage embryo Present 1A 4-cell SNAT1 , 1A 4-cell Merge Expression is cytoplasmic and nuclear.
Specimen 1A Morula: embryonic day 2.25 (more )
Note: Morula stage embryo. Double labeled: red - Slc38a1; green - Pou5f1 (assay MGI:7506229).
Structure Level Pattern Image Note
TS3: compacted morula Present Non-Uniform 1A Morula SNAT1 , 1A Morula merge There is less immunofluorescence in those blastomeres internalized during the 4th cleavage event.
Specimen 2A E3.5: embryonic day 3.5 (more )
Note: Blastocyst stage embryo. Double labeled: red - Slc38a1; green - Pou5f1 (assay MGI:7506229).
Structure Level Pattern Image Note
TS4: trophectoderm Present 2A E3.5 Merge , 2A E3.5 SNAT1
TS4: inner cell mass Weak 2A E3.5 Merge , 2A E3.5 SNAT1
Specimen 2A E4.5: embryonic day 4.5 (more )
Note: Blastocyst stage embryo. Double labeled: red - Slc38a1; green - Pou5f1 (assay MGI:7506229).
Structure Level Pattern Image Note
TS5: trophectoderm Present 2A E4.5 SNAT1 , 2A E4.5 Merge
TS5: inner cell mass Weak 2A E4.5 Merge , 2A E4.5 SNAT1
Specimen 4A: embryonic day 5.5 (more )
Structure Level Pattern Image Note
TS8: embryo Strong 4A SNAT1 right , 4A SNAT1 , 4A SNAT1 detail
TS8: extraembryonic component Present 4A SNAT1 right , 4A SNAT1 detail , 4A SNAT1
Specimen S1A 2-cell: embryonic day 1.5 (more )
Note: 2-cell embryo. Double labeled: red - Slc38a1; green - Pou5f1 (assay MGI:7506229).
Structure Level Pattern Image Note
TS2: 2-cell stage embryo Present S1A 2-cell SNAT1 , S1A 2-cell merge Expression is cytoplasmic and nuclear.
Specimen S1A 4-cell: embryonic day 2.0 (more )
Note: 4-cell embryo. Double labeled: red - Slc38a1; green - Pou5f1 (assay MGI:7506229).
Structure Level Pattern Image Note
TS3: 4-cell stage embryo Present S1A 4-cell Merge , S1A 4-cell SNAT1 Expression is cytoplasmic and nuclear.
Specimen S1A Morula: embryonic day 2.25 (more )
Note: Morula stage embryo. Double labeled: red - Slc38a1; green - Pou5f1 (assay MGI:7506229).
Structure Level Pattern Image Note
TS3: compacted morula Present S1A Morula SNAT1 , S1A Morula merge
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/14/2024
MGI 6.23 		The Jackson Laboratory