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Gene Expression Data
Assay Details
Assay
Reference: J:320624 Tan BSN, et al., Regulation of amino acid transporters in pluripotent cell populations in the embryo and in culture; novel roles for sodium-coupled neutral amino acid transporters. Mech Dev. 2016 Aug;141:32-39
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7506229
Gene symbol: Pou5f1
Gene name: POU domain, class 5, transcription factor 1
Antibody: anti-Oct3/4 (C-10; sc-5279)
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen 1A 2-cell: embryonic day 1.5 (more )
Note: 2-cell embryo. Double labeled: green - Pou5f1; red - Slc38a1 (assay MGI:7506184).
Structure Level Pattern Image Note
TS2: 2-cell stage embryo Weak 1A 2-cell merge , 1A 2-cell Oct4
Specimen 1A 4-cell: embryonic day 2.0 (more )
Note: 4-cell embryo. Double labeled: green - Pou5f1; red - Slc38a1 (assay MGI:7506184).
Structure Level Pattern Image Note
TS3: 4-cell stage embryo Trace 1A 4-cell Oct4 , 1A 4-cell Merge
Specimen 1A Morula: embryonic day 2.25 (more )
Note: Morula stage embryo. Double labeled: green - Pou5f1; red - Slc38a1 (assay MGI:7506184).
Structure Level Pattern Image Note
TS3: compacted morula Present 1A Morula merge , 1A Morula Oct4
Specimen 1B 2-cell: embryonic day 1.5 (more )
Note: 2-cell embryo. Double labeled: green - Pou5f1; red - Slc38a2 (assay MGI:7506230).
Structure Level Pattern Image Note
TS2: 2-cell stage embryo Weak 1B 2-cell Oct4 , 1B 2-cell merge
Specimen 1B 4-cell: embryonic day 2.0 (more )
Note: 4-cell embryo. Double labeled: green - Pou5f1; red - Slc38a2 (assay MGI:7506230).
Structure Level Pattern Image Note
TS3: 4-cell stage embryo Trace 1B 4-cell Merge , 1B 4-cell Oct4
Specimen 1B Morula: embryonic day 2.25 (more )
Note: Morula stage embryo. Double labeled: green - Pou5f1; red - Slc38a2 (assay MGI:7506230).
Structure Level Pattern Image Note
TS3: compacted morula Present 1B Morula Oct4 , 1B Morula Merge
Specimen 2A E3.5: embryonic day 3.5 (more )
Note: Blastocyst stage embryo. Double labeled: green - Pou5f1; red - Slc38a1 (assay MGI:7506184).
Structure Level Pattern Image Note
TS4: inner cell mass Present Regionally restricted 2A E3.5 Merge , 2A E3.5 Oct4 Expression is in the nuclei of all non-dividing cells.
Specimen 2A E4.5: embryonic day 4.5 (more )
Note: Blastocyst stage embryo. Double labeled: green - Pou5f1; red - Slc38a1 (assay MGI:7506184).
Structure Level Pattern Image Note
TS5: inner cell mass Present 2A E4.5 Oct4 , 2A E4.5 Merge Expression is nuclear.
TS5: trophectoderm Weak 2A E4.5 Oct4 , 2A E4.5 Merge
Specimen 2B E3.5i: embryonic day 3.5 (more )
Note: Early blastocyst stage embryo. Double labeled: green - Pou5f1; red - Slc38a2 (assay MGI:7506230).
Structure Level Pattern Image Note
TS4: inner cell mass Present Regionally restricted 2B E3.5i Merge , 2B E3.5i Oct4 Expression is in the nuclei of all non-dividing cells.
Specimen 2B E3.5ii: embryonic day 3.5 (more )
Note: Fully expanded blastocyst stage embryo. Double labeled: green - Pou5f1; red - Slc38a2 (assay MGI:7506230).
Structure Level Pattern Image Note
TS4: inner cell mass Present 2B E3.5ii Merge , 2B E3.5ii Oct4
Specimen 2B E4.5: embryonic day 4.5 (more )
Note: Blastocyst stage embryo. Double labeled: green - Pou5f1; red - Slc38a2 (assay MGI:7506230).
Structure Level Pattern Image Note
TS5: inner cell mass Present 2B E4.5 Oct4 , 2B E4.5 Merge Expression is nuclear.
TS5: trophectoderm Weak 2B E4.5 Oct4 , 2B E4.5 Merge
Specimen S1A 2-cell: embryonic day 1.5 (more )
Note: 2-cell embryo. Double labeled: green - Pou5f1; red - Slc38a1 (assay MGI:7506184).
Structure Level Pattern Image Note
TS2: 2-cell stage embryo Weak S1A 2-cell merge , S1A 2-cell Oct4
Specimen S1A 4-cell: embryonic day 2.0 (more )
Note: 4-cell embryo. Double labeled: green - Pou5f1; red - Slc38a1 (assay MGI:7506184).
Structure Level Pattern Image Note
TS3: 4-cell stage embryo Trace S1A 4-cell Oct4 , S1A 4-cell Merge
Specimen S1A Morula: embryonic day 2.25 (more )
Note: Morula stage embryo. Double labeled: green - Pou5f1; red - Slc38a1 (assay MGI:7506184).
Structure Level Pattern Image Note
TS3: compacted morula Present S1A Morula Oct4 , S1A Morula merge
Specimen S1B 2-cell: embryonic day 1.5 (more )
Note: 2-cell embryo. Double labeled: green - Pou5f1; red - Slc38a2 (assay MGI:7506230).
Structure Level Pattern Image Note
TS2: 2-cell stage embryo Weak S1B 2-cell Oct4 , S1B 2-cell merge
Specimen S1B 4-cell: embryonic day 2.0 (more )
Note: 4-cell embryo. Double labeled: green - Pou5f1; red - Slc38a2 (assay MGI:7506230).
Structure Level Pattern Image Note
TS3: 4-cell stage embryo Trace S1B 4-cell Oct4 , S1B 4-cell Merge
Specimen S1B Morula: embryonic day 2.25 (more )
Note: Morula stage embryo. Double labeled: green - Pou5f1; red - Slc38a2 (assay MGI:7506230).
Structure Level Pattern Image Note
TS3: compacted morula Present S1B Morula Oct4 , S1B Morula Merge
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/14/2024
MGI 6.23 		The Jackson Laboratory